Ecto-nucleotidase of cultured rat superior cervical ganglia: Dipyridamole is a novel inhibitor

European Journal of Pharmacology

Volumn 397, Issue 2-3, 2000, Pages 271-277

  Connolly, Gerald P ^a   Duley, John A ^b  

^a Purine NeuroScience Laboratory   (United Kingdom)

^b ST THOMAS HOSPITAL   (United Kingdom)

Author keywords

ATP; Dipyridamole; Ecto ATPase; Ecto nucleotidase; Superior cervical ganglion, rat; UTP

Indexed keywords

ADENOSINE; ADENOSINE TRIPHOSPHATE; BETA,GAMMA DIBROMOMETHYLENE 6 N,N DIETHYLADENOSINE TRIPHOSPHATE; CYTARABINE; DIPYRIDAMOLE; ECTO 5' NUCLEOTIDASE; NUCLEOSIDE; URIDINE TRIPHOSPHATE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CATABOLISM; CONTROLLED STUDY; ENZYME INHIBITION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NONHUMAN; NUCLEOTIDE METABOLISM; PRIORITY JOURNAL; RAT; SUPERIOR CERVICAL GANGLION;

5'-NUCLEOTIDASE; ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; ANIMALS; CELLS, CULTURED; CYTARABINE; DIMETHYLPHENYLPIPERAZINIUM IODIDE; DIPYRIDAMOLE; EXTRACELLULAR SPACE; MODELS, BIOLOGICAL; NICOTINIC AGONISTS; RATS; RATS, SPRAGUE-DAWLEY; SUPERIOR CERVICAL GANGLION; TIME FACTORS; URIDINE TRIPHOSPHATE;

EID: 0034595912     PISSN: 00142999     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0014-2999(00)00273-9     Document Type: Article

References (20)

1. Connolly G.P. Evidence from desensitisation studies for distinct receptors for ATP and UTP on the rat superior cervical ganglion. Br. J. Pharmacol. 112:1994;357-359.
2. Connolly G.P., Stone T.W. On the site of action and inactivation of adenosine by the rat superior cervical ganglion. J. Auton. Pharmacol. 13:1993;237-247.
3. Connolly G.P., Stone T.W. Adenosine selectively depresses muscarinic compared with non-muscarinic receptor mediated depolarisation of the rat superior cervical ganglion. Gen. Pharmacol. 26:1995;865-873.
4. Connolly G.P., Harrison P.J., Stone T.W. Action of purine and pyrimidine nucleotides on the rat superior cervical ganglion. Br. J. Pharmacol. 110:1993;1297-1304.
5. Connolly G.P., Abbott N.J., Demaine C., Duley J.A. Investigation of receptors responsive to pyrimidines. Trends Pharmacol. Sci. 18:1997;413-414.
6. Connolly G.P., Demaine C., Duley J.A. Ecto-nucleotidases in isolated intact rat vagi, nodose ganglia and superior cervical ganglia. Adv. Exp. Med. Biol. 431:1998;769-776.
7. Crack B.E., Pollard C.E., Beukers M.W., Roberts S.M., Hunt S.F., Ingall A.H., McKechnie K.C., Ijzerman A.P., Leff P. Pharmacological and biochemical analysis of FPL 67156, a novel, selective inhibitor of ecto-ATPase. Br. J. Pharmacol. 114:1995;475-481.
8. Cusack N.J., Hourani S.M.O., Welford L.A. The role of ectonucleotideases in the pharmacological responses to nucleotide analogues. Paton D.M. Adenosine and Nucleotides: Physiology and Pharmacology. 1988;51-58 Taylor and Francis, London.
9. Heyliger C.E., Panagia V., Dhalla N.S. Effect of cyclic AMP phosphodiesterase inhibitors on cardiac sarcolemmal 5′-nucleotidase. J. Pharmacol. Exp. Ther. 217:1981;489-493.
10. Lai K.M., Wong P.C. Metabolism of extracellular adenine nucleotides by cultured rat brain astrocytes. J. Neurochem. 57:1991;1510-1515.
11. Lazarowski E.R., Homolya L., Boucher R.C., Harden T.K. Identification of an ecto-nucleoside diphosphokinase and its contribution to interconversion of P2 receptor agonists. J. Biol. Chem. 272:1997;20402-20407.
12. Nacimento W., Kreutzberg G.W. Cytochemistry of 5′-nucleotidase in the superior cervical ganglion of the rat: effects of pre- and postganglionic axotomy. Expt. Neurol. 109:1990;362-373.
13. Simmonds H.A., Duley J.A., Davies P.M. Analysis of purines and pyrimidines in blood, urine, and other physiological fluids. Techniques. Holmes F.A. Diagnostic Human Biochemical Genetics: A Laboratory Manual. 1991;397-424 Wiley-Liss, London.
14. Smith T.M., Lewis Carl S.A., Kirley T.L. Mutagenesis of two conserved tryptophan residues of the E-type ATPases: inactivation and conversion of an ecto-apyrase to an ecto-NTPase. Biochemistry. 38:1999;5849-5857.
15. Stefanovic V., Ledig M., Mandel P. Divalent cation-activated ecto-nucleoside triphosphatase activity of nervous system cells in tissue culture. J. Neurochem. 27:1976;799-805.
16. Tennant J.P., Callagham F., Turner C., Hourani S.M.O. Effects of allopurinol, erythro-9-(2-hydroxy-3-nonyl)-adenine and S-(4-nitrobenzyl)-6-thioinosine on the degradation of adenosine 5′-triphosphate on the rat colon muscaularis mucosae. J. Auton. Pharmacol. 19:1999;229-232.
17. Vizi E.S., Liang S.D., Sperlagh B., Kittel A., Juranyi Z. Studies on the release and extracellular metabolism of endogenous ATP in rat superior cervical ganglion: support for neurotransmitter role of ATP. Neuroscience. 79:1997;983-993.
18. Weishaar R.E., Cain M.H., Bristol J.A. A new generation of phosphodiesterase inhibitors: multiple molecular forms of phosphodiesterase and the potential for drug selectivity. J. Med. Chem. 28:1985;537-545.
19. Welford L.A., Cusack N.J., Hourani S.M. ATP analogues and the guinea-pig taenia coli: a comparison of the structure-activity relationships of ectonucleotidases with those of the P2-purinoceptor. Eur. J. Pharmacol. 129:1986;217-224.
20. Wolinsky E.J., Patterson P.H. Potassium-stimulated purine release by cultured sympathetic neurons. J. Neurosci. 5:1985;1680-1687.