-
1
-
-
0023461268
-
Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction
-
Mullis KB, Faloona FA. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol 1987 ; 155 : 335-50.
-
(1987)
Methods Enzymol
, vol.155
, pp. 335-350
-
-
Mullis, K.B.1
Faloona, F.A.2
-
2
-
-
0025115739
-
Quantitative analysis of MDR1 (multidrug resistance) gene expression in human tumors by polymerase chain reaction
-
Noonan KE, Beck C, Holzmayer TA, et al. Quantitative analysis of MDR1 (multidrug resistance) gene expression in human tumors by polymerase chain reaction. Proc Natl Acad Sci USA 1990 ; 87 : 7160-4.
-
(1990)
Proc Natl Acad Sci USA
, vol.87
, pp. 7160-7164
-
-
Noonan, K.E.1
Beck, C.2
Holzmayer, T.A.3
-
3
-
-
0024338294
-
Detection of amplified oncogenes by differential polymerase chain reaction
-
Frye RR, Benz CC, Liu E. Detection of amplified oncogenes by differential polymerase chain reaction. Oncogene 1989 ; 4 : 1153-7.
-
(1989)
Oncogene
, vol.4
, pp. 1153-1157
-
-
Frye, R.R.1
Benz, C.C.2
Liu, E.3
-
5
-
-
0029778731
-
Technical aspects of quantitative competitive PCR
-
Zimmermann K, Mannhalter JW. Technical aspects of quantitative competitive PCR. Biotechniques 1996 ; 21 : 268-79.
-
(1996)
Biotechniques
, vol.21
, pp. 268-279
-
-
Zimmermann, K.1
Mannhalter, J.W.2
-
6
-
-
15744381713
-
Kinetic PCR analysis: Real time monitoring of DNA amplification reactions
-
Higuchi R, Fockler C, Dollinger G, Watson R. Kinetic PCR analysis: real time monitoring of DNA amplification reactions. Biotechnology 1993 ; 11 : 1026-30.
-
(1993)
Biotechnology
, vol.11
, pp. 1026-1030
-
-
Higuchi, R.1
Fockler, C.2
Dollinger, G.3
Watson, R.4
-
7
-
-
0002559310
-
General aspects and chances of nucleic acid quantitation by PCR
-
Köhler T, Laßner D, Rost AK, Thamm B, Pustowoit B, Remke H, eds. Springer
-
Köhler T. General aspects and chances of nucleic acid quantitation by PCR. In: Köhler T, Laßner D, Rost AK, Thamm B, Pustowoit B, Remke H, eds. Quantitation of mRNA by polymerase chain reaction. Springer 1992 : 1-26.
-
(1992)
Quantitation of mRNA by Polymerase Chain Reaction
, pp. 1-26
-
-
Köhler, T.1
-
8
-
-
0027996969
-
The impact of the PCR plateau phase on quantitative PCR
-
Morrison C, Gannon F. The impact of the PCR plateau phase on quantitative PCR. Biochim Biophys Acta 1994 ; 1219 : 493-8.
-
(1994)
Biochim Biophys Acta
, vol.1219
, pp. 493-498
-
-
Morrison, C.1
Gannon, F.2
-
11
-
-
14744306436
-
Simultaneous amplification and detection of specific DNA sequences
-
Higuchi R, Dollinger G, Walsh PS, Griffith R. Simultaneous amplification and detection of specific DNA sequences. Biotechnology 1992 ; 10 : 413-7.
-
(1992)
Biotechnology
, vol.10
, pp. 413-417
-
-
Higuchi, R.1
Dollinger, G.2
Walsh, P.S.3
Griffith, R.4
-
12
-
-
0031568919
-
Product differentiation by analysis of DNA melting curves during the polymerase chain reaction
-
Ririe KM, Rasmussen RP, Wittwer CT. Product differentiation by analysis of DNA melting curves during the polymerase chain reaction. Anal Biochem 1997; 245 : 154-60.
-
(1997)
Anal Biochem
, vol.245
, pp. 154-160
-
-
Ririe, K.M.1
Rasmussen, R.P.2
Wittwer, C.T.3
-
13
-
-
0028924992
-
Fluorescence resonance energy transfer
-
Clegg RM. Fluorescence resonance energy transfer. Curr Opin Biotechnol 1995 ; 6 : 103-10.
-
(1995)
Curr Opin Biotechnol
, vol.6
, pp. 103-110
-
-
Clegg, R.M.1
-
14
-
-
0031030886
-
Energy-transfer fluorescent reagents for DNA analyses
-
Glazer AN, Mathies RA. Energy-transfer fluorescent reagents for DNA analyses. Curr Opin Biotechnol 1997 ; 8 : 94-102.
-
(1997)
Curr Opin Biotechnol
, vol.8
, pp. 94-102
-
-
Glazer, A.N.1
Mathies, R.A.2
-
15
-
-
0028834086
-
Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization
-
Livak KJ, Flood SJA, Marmaro J, Giusti W, Deetz K. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. PCR Methods Appl 1995 ; 4 : 357-62.
-
(1995)
PCR Methods Appl
, vol.4
, pp. 357-362
-
-
Livak, K.J.1
Flood, S.J.A.2
Marmaro, J.3
Giusti, W.4
Deetz, K.5
-
16
-
-
0002464272
-
LightCycler™ hybridization probes. The most direct way to monitor PCR amplification for quantitation and mutation detection
-
Caplin BE, Rasmussen RP, Bernard PS, Wittwer CT. LightCycler™ hybridization probes. The most direct way to monitor PCR amplification for quantitation and mutation detection. Roche Mol Biochem Biochim 1999 ; 1 : 5-8.
-
(1999)
Roche Mol Biochem Biochim
, vol.1
, pp. 5-8
-
-
Caplin, B.E.1
Rasmussen, R.P.2
Bernard, P.S.3
Wittwer, C.T.4
-
17
-
-
0029670262
-
Molecular beacons: Probes that fluoresce upon hybridization
-
Tyagi S, Kramer FR. Molecular beacons: probes that fluoresce upon hybridization. Nat Biotechnol 1996 ; 14 : 303-8.
-
(1996)
Nat Biotechnol
, vol.14
, pp. 303-308
-
-
Tyagi, S.1
Kramer, F.R.2
-
18
-
-
0032839195
-
Detection of PCR products using self-probing amplicons and fluorescence
-
Whitcombe D, Theaker J, Guy SP, Brown T, Little S. Detection of PCR products using self-probing amplicons and fluorescence. Nat Biotechnol 1999 ; 17 : 804-7.
-
(1999)
Nat Biotechnol
, vol.17
, pp. 804-807
-
-
Whitcombe, D.1
Theaker, J.2
Guy, S.P.3
Brown, T.4
Little, S.5
-
19
-
-
0030871285
-
A closed tube format for amplification and detection of DNA based on energy transfer
-
Nazarenko IA, Bhatnagar SK, Hohman RJ. A closed tube format for amplification and detection of DNA based on energy transfer. Nucl Acids Res 1997 ; 25 : 2516-21.
-
(1997)
Nucl Acids Res
, vol.25
, pp. 2516-2521
-
-
Nazarenko, I.A.1
Bhatnagar, S.K.2
Hohman, R.J.3
-
20
-
-
0034544582
-
Detection of PCR products in real time using light-up probes
-
Svanvik N, Stählberg A, Sehlstedt U, Sjöback R, Kubista M. Detection of PCR products in real time using light-up probes. Anal Biochem 2000 ; 287 : 179-82.
-
(2000)
Anal Biochem
, vol.287
, pp. 179-182
-
-
Svanvik, N.1
Stählberg, A.2
Sehlstedt, U.3
Sjöback, R.4
Kubista, M.5
-
21
-
-
0025861818
-
Detection of specific polymerase chain reaction product by utilizing the 5′ to 3′ exonuclease activity of Thermus aquaticus DNA polymerase
-
Holland PM, Abramson RD, Watson R, Gelfand DH. Detection of specific polymerase chain reaction product by utilizing the 5′ to 3′ exonuclease activity of Thermus aquaticus DNA polymerase. Proc Natl Acad Sci USA 1991 ; 88 : 7276-80.
-
(1991)
Proc Natl Acad Sci USA
, vol.88
, pp. 7276-7280
-
-
Holland, P.M.1
Abramson, R.D.2
Watson, R.3
Gelfand, D.H.4
-
22
-
-
0034075378
-
Influence of DNA polymerases on quantitative PCR results using TaqMan™ probe format in the LightCycler™ instrument
-
Kreuzer KA, Bohn A, Lass U, Peters UR, Schmidt CA. Influence of DNA polymerases on quantitative PCR results using TaqMan™ probe format in the LightCycler™ instrument. Mol Cell Probes 2000 ; 14 : 57-60.
-
(2000)
Mol Cell Probes
, vol.14
, pp. 57-60
-
-
Kreuzer, K.A.1
Bohn, A.2
Lass, U.3
Peters, U.R.4
Schmidt, C.A.5
-
23
-
-
0034650608
-
3′-Minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures
-
Kutyavin IV, Afonina IA, Mills A, et al. 3′-Minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures. Nucl Acids Res 2000 ; 28 : 655-61.
-
(2000)
Nucl Acids Res
, vol.28
, pp. 655-661
-
-
Kutyavin, I.V.1
Afonina, I.A.2
Mills, A.3
-
24
-
-
0032804911
-
Simultaneous detection of TaqMan probes containing Fam and Tamra reporter fluorophores
-
Nasarabadi S, Milanovitch F, Richards J, Belgrader P. Simultaneous detection of TaqMan probes containing Fam and Tamra reporter fluorophores. Biotechniques 1999 ; 27 : 1116-8.
-
(1999)
Biotechniques
, vol.27
, pp. 1116-1118
-
-
Nasarabadi, S.1
Milanovitch, F.2
Richards, J.3
Belgrader, P.4
-
25
-
-
0002825794
-
Using molecular beacons to monitor PCR product formation
-
Cayouette M, Sucharczuk A, Moores J, Tyagi S, Kramer FR. Using molecular beacons to monitor PCR product formation. Stratagene 1999 ; 12 : 85-9.
-
(1999)
Stratagene
, vol.12
, pp. 85-89
-
-
Cayouette, M.1
Sucharczuk, A.2
Moores, J.3
Tyagi, S.4
Kramer, F.R.5
-
26
-
-
0001873780
-
Determination of target copy number of quantitative standards used in PCR-based diagnostics assays
-
Ferré F, ed. Birkhaüser
-
Wang Z, Spadoro J. Determination of target copy number of quantitative standards used in PCR-based diagnostics assays. In: Ferré F, ed. Gene quantification. Birkhaüser, 1998 : 31-43.
-
(1998)
Gene Quantification
, pp. 31-43
-
-
Wang, Z.1
Spadoro, J.2
-
27
-
-
0035713768
-
Quantitation of viral load using real-time amplification techniques
-
Niesters HG. Quantitation of viral load using real-time amplification techniques. Methods 2001 ; 25 : 419-29.
-
(2001)
Methods
, vol.25
, pp. 419-429
-
-
Niesters, H.G.1
-
28
-
-
0033775615
-
Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays
-
Bustin SA. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol 2000 ; 25 : 169-93.
-
(2000)
J Mol Endocrinol
, vol.25
, pp. 169-193
-
-
Bustin, S.A.1
-
29
-
-
0032883806
-
Housekeeping genes as internal standards: Use and limits
-
Thellin O, Zorzi W, Lakaye B, et al. Housekeeping genes as internal standards: use and limits. J Biotechnol 1999 ; 75 : 291-5.
-
(1999)
J Biotechnol
, vol.75
, pp. 291-295
-
-
Thellin, O.1
Zorzi, W.2
Lakaye, B.3
-
30
-
-
0037129827
-
Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
-
Vandesompele J, De Preter K, Pattyn F, et al. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 2002 ; 3 : 0034.1-11.
-
(2002)
Genome Biol
, vol.3
, pp. 00341-11
-
-
Vandesompele, J.1
De Preter, K.2
Pattyn, F.3
-
31
-
-
17344392308
-
A new mathematical model for relative quantification in real-time RT-PCR
-
Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 2001 ; 29 : 2002-7.
-
(2001)
Nucleic Acids Res
, vol.29
, pp. 2002-2007
-
-
Pfaffl, M.W.1
-
32
-
-
0035003174
-
A novel method to compensate for different amplification efficiencies between patient DNA samples in quantitative real-time PCR
-
Meijerink J, Mandigers C, Van de Locht L, Tönnissen E, Goodsaid F, Raemaekers J. A novel method to compensate for different amplification efficiencies between patient DNA samples in quantitative real-time PCR. J Mol Diag 2001 ; 3 : 55-61.
-
(2001)
J Mol Diag
, vol.3
, pp. 55-61
-
-
Meijerink, J.1
Mandigers, C.2
Van De Locht, L.3
Tönnissen, E.4
Goodsaid, F.5
Raemaekers, J.6
-
33
-
-
0002129975
-
Quantification on the LightCycler
-
Meuer S, Wittwer C, Nakagawara K, eds. Heidelberg: Springer Press
-
Rasmussen R. Quantification on the LightCycler. In: Meuer S, Wittwer C, Nakagawara K, eds. Rapid cycle real-time PCR, methods and applications. Heidelberg: Springer Press, 2001 ; 21-34.
-
(2001)
Rapid Cycle Real-Time PCR, Methods and Applications
, pp. 21-34
-
-
Rasmussen, R.1
-
34
-
-
0036278859
-
Gene quantification using real-time quantitative PCR: An emerging technology hits the mainstream
-
Ginzinger DG. Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream. Exp Hematol 2002 ; 30 : 503-12.
-
(2002)
Exp Hematol
, vol.30
, pp. 503-512
-
-
Ginzinger, D.G.1
-
35
-
-
0036320413
-
Real-time PCR technology for cancer diagnostics
-
Bernard PS, Wittwer CT. Real-time PCR technology for cancer diagnostics. Clin Chem 2002 ; 48 : 1178-85.
-
(2002)
Clin Chem
, vol.48
, pp. 1178-1185
-
-
Bernard, P.S.1
Wittwer, C.T.2
-
36
-
-
0035710229
-
An overview of real-time quantitative PCR: Applications to quantify cytokine gene expression
-
Giulietti A, Overbergh L, Valckx D, Decallonne B, Bouillon R, Mathieu C. An overview of real-time quantitative PCR: applications to quantify cytokine gene expression. Methods 2001 ; 25 : 386-401.
-
(2001)
Methods
, vol.25
, pp. 386-401
-
-
Giulietti, A.1
Overbergh, L.2
Valckx, D.3
Decallonne, B.4
Bouillon, R.5
Mathieu, C.6
-
37
-
-
0035057561
-
Validation of array-based gene expression profiles by real-time (kinetic) RT-PCR
-
Rajeevan MS, Vernon SD, Taysavang N, Unger ER. Validation of array-based gene expression profiles by real-time (kinetic) RT-PCR. J Mol Diagn 2001 ; 3 : 26-31.
-
(2001)
J Mol Diagn
, vol.3
, pp. 26-31
-
-
Rajeevan, M.S.1
Vernon, S.D.2
Taysavang, N.3
Unger, E.R.4
|