Influence of DNA polymerases on quantitative PCR results using TaqMan(TM) probe format in the LightCycler(TM) instrument

Molecular and Cellular Probes

Volumn 14, Issue 2, 2000, Pages 57-60

  Kreuzer, K A ^a   Bohn, A ^a   Lass, U ^a   Peters, U R ^a   Schmidt, C A ^a  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

5' exonuclease activity; DNA polymerases; Fluorescent probes; Real time PCR

Indexed keywords

DNA POLYMERASE; EXONUCLEASE;

ARTICLE; FLUORESCENCE ANALYSIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

EID: 0034075378     PISSN: 08908508     EISSN: None     Source Type: Journal    
DOI: 10.1006/mcpr.1999.0284     Document Type: Article

References (4)

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2. Wittwer C. T., Ririe K. M., Andrew R. V., David D. A., Gundry R. A., Balis U. J. The LightCycler: a microvolume multisample fluorimeter with rapid temperature control. Biotechniques. 22:1997;176-181.
3. Rychlik W. Priming efficiency in PCR. Biotechniques. 23:1995;714-720.
4. Lyamichev V., Brow M. A., Dahlberg E. Structure-specific endonucleolytic cleavage of nucleic acids by eubacterial DNA polymerases. Science. 260:1993;778-783.