The molecular basis of the expansive substrate specificity of the antibiotic resistance enzyme aminoglycoside acetyltransferase-6′-aminoglycoside phosphotransferase-2′: The role of Asp-99 as an active site base important for acetyl transfer

Journal of Biological Chemistry

Volumn 278, Issue 15, 2003, Pages 12873-12880

  Boehr, David D ^a   Jenkins, Stephen I ^a   Wright, Gerard D ^a  

^a MCMASTER UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ACETYLATION; ANTIBODIES; BACTERIA; CATALYSIS; ENZYMES; SUBSTRATES;

BIFUNCTIONAL ENZYMES;

BIOCHEMISTRY;

ACETYL COENZYME A; AMINOGLYCOSIDE ACETYLTRANSFERASE:6' AMINOGLYCOSIDE PHOSPHOTRANSFERASE 2''; AMINOGLYCOSIDE ANTIBIOTIC AGENT; ASTROMICIN; KANAMYCIN A; NEAMINE; PAROMOMYCIN; PHENANTHRENE DERIVATIVE; PHOSPHOTRANSFERASE; UNCLASSIFIED DRUG; 6' AMINOGLYCOSIDE ACETYLTRANSFERASE 2'' AMINOGLYCOSIDE PHOSPHOTRANSFERASE; 6'-AMINOGLYCOSIDE ACETYLTRANSFERASE-2''-AMINOGLYCOSIDE PHOSPHOTRANSFERASE; ACYLTRANSFERASE; ASPARTIC ACID; PRIMER DNA; RECOMBINANT PROTEIN;

ACETYLATION; ANTIBIOTIC RESISTANCE; ARTICLE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME SPECIFICITY; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BINDING SITE; CHEMISTRY; GENETICS; KINETICS; METABOLISM; MOLECULAR GENETICS; PH; PROTEIN CONFORMATION; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY;

BACTERIA (MICROORGANISMS); POSIBACTERIA;

ACETYLTRANSFERASES; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ASPARTIC ACID; BINDING SITES; DNA PRIMERS; HYDROGEN-ION CONCENTRATION; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PHOSPHOTRANSFERASES (ALCOHOL GROUP ACCEPTOR); PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY;

EID: 0038305950     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M211680200     Document Type: Article

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