Functional dissection of the Bacillus subtilis pur operator site

Journal of Bacteriology

Volumn 185, Issue 14, 2003, Pages 4099-4109

  Bera, Aloke Kumar ^a   Zhu, Jianghai ^a   Zalkin, Howard ^b   Smith, Janet L ^a  

^a PURDUE UNIVERSITY   (United States)

^b PURDUE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GENE PRODUCT; PHOSPHORIBOSYL PYROPHOSPHATE; PROTEIN; PUR R PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS SUBTILIS; DIMERIZATION; DNA BINDING; DNA FLANKING REGION; GENE EXPRESSION REGULATION; MOLECULAR RECOGNITION; NONHUMAN; NUCLEOTIDE REPEAT; OPERATOR GENE; PRIORITY JOURNAL; PROMOTER REGION; PURINE METABOLISM; REGULON; TRANSCRIPTION REGULATION;

BACILLUS SUBTILIS; BACTERIAL PROTEINS; BASE SEQUENCE; BINDING SITES; CRYSTALLOGRAPHY, X-RAY; CYCLIC AMP RESPONSE ELEMENT-BINDING PROTEIN; DNA, BACTERIAL; DNA-BINDING PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; MOLECULAR SEQUENCE DATA; OPERATOR REGIONS (GENETICS); OPERON; PHOSPHORIBOSYL PYROPHOSPHATE; PURINES; REPRESSOR PROTEINS; TRANSCRIPTION FACTORS;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS);

EID: 0038261974     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.185.14.4099-4109.2003     Document Type: Article

References (20)

1. Ebbole, D. J., and H. Zalkin. 1989. Bacillus subtilis pur operon expression and regulation. J. Bacteriol. 171:2136-2141.
2. Ebbole, D. J., and H. Zalkin. 1989. Interaction of a putative repressor protein with an extended control region of the Bacillus subtilis pur operon. J. Biol. Chem. 264:3553-3561.
3. Gajiwala, K. S., and S. K. Burley. 2000. Winged helix proteins. Curr. Opin. Struct. Biol. 10:110-116.
4. Kilstrup, M., S. G. Jessing, S. B. Wichmand-Jørgensen, M. Madsen, and D. Nilsson. 1998. Activation control of pur gene expression in Lactococcus lactis: proposal for a consensus activator binding sequence based on deletion analysis and site-directed mutagenesis of purC and purD promoter regions. J. Bacteriol. 180:3900-3906.
5. Kilstrup, M., and J. Martinussen. 1998. A transcriptional activator, homologous to the Bacillus subtilis PurR repressor, is required for expression of purine biosynthetic genes in Lactococcus lactis. J. Bacteriol. 180:3907-3916.
6. Krahn, J. M., J. H. Kim, M. R. Burns, R. J. Parry, H. Zalkin, and J. L. Smith. 1997. Coupled formation of an amidotransferase interdomain ammonia channel and a phosphoribosyltransferase active site. Biochemistry 36:11061-11068.
7. Murshudov, G. N., A. A. Vagin, and E. J. Dodson. 1997. Refinement of macromolecular structures by the maximum-likelihood method. Acta Crystallogr. D 53:240-255.
8. Navaza, J. 1994. AMoRe - an automated package for molecular replacement. Acta Crystallogr. A 50:157-163.
9. Nelson, H. C., J. T. Finch, B. F. Luisi, and A. Klug. 1987. The structure of an oligo(dA).oligo(dT) tract and its biological implications. Nature 330:221-226.
10. Nygaard, P. 1993. Purine and pyrimidine salvage pathways, p. 359-378. In A. L. Sonenstein, J. A. Hoch, and R. Losick (ed.), Bacillus subtilis and other gram-positive bacteria: biochemistry, physiology, and molecular genetics. ASM Press, Washington D.C.
11. Otwinowski, Z., and W. Minor. 1997. Processing of X-ray diffraction data collected in oscillation mode., Methods Enzymol. 276:307-325.
12. Rappu, P., B. S. Shin, H. Zalkin, and P. Mäntsälä. 1999. A role for a highly conserved protein of unknown function in regulation of Bacillus subtilis purA by the purine repressor. J. Bacteriol. 181:3810-3815.
13. Saxild, H. H., K. Brunstedt, K. I. Nielsen, H. Jarmer, and P. Nygaard. 2001. Definition of the Bacillus subtilis PurR operator using genetic and bioinformatic tools and expansion of the PurR regulon with glyA, guaC, pbuG, xpt-pbuX, yqhZ-folD, and pbuO. J. Bacteriol. 183:6175-6183.
14. Schumacher, M. A., K. Y. Choi, H. Zalkin, and R. G. Brennan. 1994. Crystal structure of LacI member, PurR, bound to DNA: minor groove binding by α helices. Science 266:763-770.
15. Shin, B. S., A. Stein, and H. Zalkin. 1997. Interaction of Bacillus subtilis purine repressor with DNA. J. Bacteriol. 179:7394-7402.
16. Sinha, S., J. M. Krahn, B. S. Shin, D. R. Tomchick, H. Zalkin, and J. L. Smith. 2003. The purine repressor of Bacillus subtilis: a novel combination of domains adapted for transcription regulation. J. Bacteriol. 185:4087-4098.
17. Sinha, S. C., and J. L. Smith. 2001. The PRT protein family. Curr. Opin. Struct. Biol. 11:733-739.
18. Tan, S., Y. Hunziker, L. Pellegrini, and T. J. Richmond. 2000. Crystallization of the yeast MATα2/MCM1/DNA ternary complex: general methods and principles for protein/DNA cocrystallization. J. Mol. Biol. 297:947-959.
19. Weng, M., P. L. Nagy, and H. Zalkin. 1995. Identification of the Bacillus subtilis pur operon repressor. Proc. Natl. Acad. Sci. USA 92:7455-7459.
20. Weng, M., and H. Zalkin. 2000. Mutations in the Bacillus subtilis purine repressor that perturb PRPP effector function in vitro and in vivo. Curr. Microbiol. 41:56-59.