A transcriptional activator, homologous to the Bacillus subtilis PurR repressor, is required for expression of purine biosynthetic genes in Lactococcus lactis

Journal of Bacteriology

Volumn 180, Issue 15, 1998, Pages 3907-3916

  Kilstrup, Mogens ^a   Martinussen, Jan ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; BETA GALACTOSIDASE; PHOSPHORIBOSYL PYROPHOSPHATE; PURINE;

ARTICLE; AUXOTROPHY; BACILLUS SUBTILIS; CONSENSUS SEQUENCE; CONTROLLED STUDY; GENE EXPRESSION; LACTOCOCCUS LACTIS; MUTANT; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PURINE SYNTHESIS; REPRESSOR GENE; TRANSACTIVATION;

BACILLUS SUBTILIS;

EID: 0031877250     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.180.15.3907-3916.1998     Document Type: Article

References (41)

1. Arnau, J., K. I. Sørensen, K. F. Appel, F. K. Vogensen, and K. Hammer. 1996. Analysis of heat shock gene expression in Lactococcus lactis MG1363. Microbiology 142:1685-1691.
2. Arnvig, K., B. Hove-Jensen, and R. L. Switzer. 1990. Purification and properties of phosphoribosyl-diphosphate synthetase from Bacillus subtilis. Eur. J. Biochem. 192:195-200.
3. Casadaban, M. J., and S. N. Cohen. 1980. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J. Mol. Biol. 138:179-207.
4. Christiansen, L. C., S. Schou, P. Nygaard, and H. H. Saxild. 1997. Xanthine metabolism in Bacillus subtilis: characterization of the xpt-pbuX operon and evidence for purine- and nitrogen-controlled expression of genes involved in xanthine salvage and catabolism. J. Bacteriol. 179:2540-2550.
5. Cregg, K. M., I. Wilding, and M. T. Black. 1998. Molecular cloning and expression of the spsB gene encoding an essential type I signal peptidase from Staphylococcus aureus. J. Bacteriol. 178:5712-5718.
6. Ebbole, D. J., and H. Zalkin. 1987. Cloning and characterization of a 12-gene cluster from Bacillus subtilis encoding nine enzymes for de novo purine nucleotide synthesis. J. Biol. Chem. 262:8274-8287.
7. Ebbole, D. J., and H. Zalkin. 1988. Characterization of the Bacillus subtilis pur operon: new insights into gene regulation, p. 51-55. In J. A. Hoch (ed.), Genetics and biotechnology of bacilli, vol. 2. Academic Press, Inc., New York, N.Y.
8. Ebbole, D. J., and H. Zalkin. 1989. Interaction of a putative repressor protein with an extended control region of the Bacillus subtilis pur operon. J. Biol. Chem. 264:3553-3561.
9. Gasson, M. J. 1983. Plasmid complements of Streptococcus lactis NCDO 712 and other lactic streptococci after protoplast-induced curing. J. Bacteriol. 154:1-9.
10. Hayes, F., C. Daly, and G. F. Fitzgerald. 1990. Identification of the minimal replicon of Lactococcus lactis subsp. lactis UC317 plasmid pCI305. Appl. Environ. Microbiol. 56:202-219.
11. He, B., A. Shiau, K. Y. Choi, H. Zalkin, and J. M. Smith. 1990. Genes of the Escherichia coli pur regulon are negatively controlled by a repressor-operator interaction. J. Bacteriol. 172:4555-4562.
12. Henriksen, A., N. Aghajari, K. F. Jensen, and M. Gajhede. 1996. A flexible loop at the dimer interface is part of the active site of the adjacent monomer of Escherichia coli orotate phosphoribosyltransferase. Biochemistry 35:3803-3809.
13. Holo, H., and I. F. Nes. 1995. Transformation of Lactococcus by electroporation. Methods Mol. Biol. 47:195-199.
14. Hove-Jensen, B., K. W. Harlow, C. J. King, and R. L. Switzer. 1986. Phosphoribosylpyrophosphate synthetase of Escherichia coli. Properties of the purified enzyme and primary structure of the prs gene. J. Biol. Chem. 261: 6765-6771.
15. Israelsen, H., S. M. Madsen, A. Vrang, E. B. Hansen, and E. Johansen. 1995. Cloning and partial characterization of regulated promoters from Lactococcus lactis Tn917-lacZ integrants with the new promoter probe vector, pAK80. Appl. Environ. Microbiol. 61:2540-2547.
16. Jensen, P. R., and K. Hammer. 1993. Minimal requirements for exponential growth of Lactococcus lactis. Appl. Environ. Microbiol. 59:4363-4366.
17. Johansen, E., and A. Kibenich. 1992. Isolation and characterization of IS1165, an insertion sequence of Leuconostoc mesenteroides subsp. cremoris and other lactic acid bacteria. Plasmid 27:200-206.
18. Kilstrup, M., S. G. Jessing, S. B. Wichmand-Jergensen, M. Madsen, and D. Nilsson. 1998. Activation control of pur gene expression in Lactococcus lactis: proposal for a consensus activator binding sequence based on deletion analysis and site-directed mutagenesis of purC and purD promoter regions. J. Bacteriol. 180:3900-3906.
19. Le Bourgeois, P., M. Lautier, M. Mata, and P. Ritzenthaler. 1992. New tools for the physical and genetic mapping of Lactococcus strains. Gene 111:109-114.
20. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265-275.
21. Maguin, E., H. Prévost, S. D. Ehrlich, and A. Grass. 1996. Efficient insertional mutagenesis in lactococci and other gram-positive bacteria. J. Bacteriol. 178:931-935.
22. Mäntsälä, P., and H. Zalkin. 1992. Cloning and sequence of Bacillus subtilis purA and guaA, involved in the conversion of IMP to AMP and GMP. J. Bacteriol. 174:1883-1890.
23. Meng, L. M., M. Kilstrup, and P. Nygaard. 1990. Autoregulation of PurR repressor synthesis and involvement of purR in the regulation of purB, purC, purL, purMN and guaBA expression in Escherichia coli. Eur. J. Biochem. 187:373-379.
24. Meng, L. M., and P. Nygaard. 1990. Identification of hypoxanthine and guanine as the corepressors for the purine regulon genes of Escherichia coli. Mol. Microbiol. 4:2187-2192.
25. Miller, J. H. 1972. Assay of β-galactosidase, p. 352-355. In J. H. Miller (ed.), Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
26. Nilsson, D., and M. Kilstrup. Cloning and expression of the Lactococcus lactis purDEK genes, required for growth on milk. Submitted for publication.
27. Ozturk, D. H., R. H. Dorfman, G. Scapin, J. C. Sacchettini, and C. Grubmeyer. 1998. Locations and functional roles of conserved lysine residues in Salmonella typhimurium orotate phosphoribosyltransferases. Biochemistry 34: 10755-10763.
28. Ozturk, D. H., R. H. Dorfman, G. Scapin, J. C. Sacchettini, and C. Grubmeyer. 1998. Structure and function of Salmonella typhimurium orotate phosphoribosyltransferase: protein complementation reveals shared active sites. Biochemistry 34:10764-10770.
29. Rolfes, R. J., and H. Zalkin. 1988. Escherichia coli gene purR encoding a repressor protein for purine nucleotide synthesis. J. Biol. Chem. 263:19653-19661.
30. Rolfes, R. J., and H. Zalkin. 1990. Purification of the Escherichia coli purine regulon repressor and identification of corepressors. J. Bacteriol. 172:5637-5642.
31. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
32. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
33. Schell, M. A. 1993. Molecular biology of the LysR family of transcriptional activators. Annu. Rev. Microbiol. 47:597-626.
34. Schumacher, M. A., K. Y. Choi, H. Zalkin, and R. G. Brennan. 1994. Crystal structure of Lad member, PurR, bound to DNA: minor groove binding by alpha helices. Science 266:763-770.
35. Shin, B. S., A. Stein, and H. Zalkin. 1997. Interaction of Bacillus subtilis purine repressor with DNA. J. Bacteriol. 179:7394-7402.
36. Terzaghi, B. E., and W. E. Sandine. 1975. Improved medium for lactic streptococci and their bacteriophages. Appl. Microbiol. 29:807-813.
37. Vieira, J., and J. Messing. 1982. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259-268.
38. Wendrich, T. M., and M. A. Marahiel. 1997. Cloning and characterization of a relA/spoT homologue from Bacillus subtilis. Mol. Microbiol. 26:65-79.
39. Weng, M., P. L. Nagy, and H. Zalkin. 1995. Identification of the Bacillus subtilis pur operon repressor. Proc. Natl. Acad. Sci. USA 92:7455-7459.
40. Youngman, P., J. B. Perkins, and R. Losick. 1984. A novel method for the rapid cloning in Escherichia coli of Bacillus subtilis chromosomal DNA adjacent to Tn917 insertions. Mol. Gen. Genet. 195:424-433.
41. Zalkin, H., and J. E. Dixon. 1992. De novo purine nucleotide biosynthesis. Prog. Nucleic Acid Res. Mol. Biol., p. 258-287.