Activation control of pur gene expression in Lactococcus lactis: Proposal for a consensus activator binding sequence based on deletion analysis and site-directed mutagenesis of purC and purD promoter regions

Journal of Bacteriology

Volumn 180, Issue 15, 1998, Pages 3900-3906

  Kilstrup, Mogens ^a   Jessing, Stine G ^a   Wichmand Jørgensen, Stephanie B ^a   Madsen, Mette ^b   Nilsson, Dan ^b  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^b CHR HANSEN A S   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

ADENINE; BACTERIAL DNA; GUANOSINE; HYPOXANTHINE; PURINE;

ARTICLE; CONSENSUS SEQUENCE; CONTROLLED STUDY; GENE DELETION; GENE EXPRESSION REGULATION; LACTOCOCCUS LACTIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROMOTER REGION; REPRESSOR GENE; SITE DIRECTED MUTAGENESIS;

LACTOCOCCUS LACTIS;

EID: 0031857910     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.180.15.3900-3906.1998     Document Type: Article

References (36)

1. Christiansen, L. C., S. Schou, P. Nygaard, and H. H. Saxild. 1997. Xanthine metabolism in Bacillus subtilis: characterization of the xpt-pbuX operon and evidence for purine- and nitrogen-controlled expression of genes involved in xanthine salvage and catabolism. J. Bacteriol. 179:2540-2550.
2. Dandanell, G., and K. Hammer. 1985. Two operator sites separated by 599 base pairs are required for deoR repression of the deo operon of Escherichia coli. EMBO J. 4:3333-3338.
3. Dickely, F., D. Nilsson, E. B. Hansen, and E. Johansen. 1995. Isolation of Lactococcus lactis nonsense suppressors and construction of a food-grade cloning vector. Mol. Microbiol. 15:839-847.
4. Ebbole, D. J., and H. Zalkin. 1987. Cloning and characterization of a 12-gene cluster from Bacillus subtilis encoding nine enzymes for de novo purine nucleotide synthesis. J. Biol. Chem. 262:8274-8287.
5. Ebbole, D. J., and H. Zalkin. 1989. Bacillus subtilis pur operon expression and regulation. J. Bacteriol. 171:2136-2141.
6. Gasson, M. J. 1983. Plasmid complements of Streptococcus lactis NCDO 712 and other lactic streptococci after protoplast-induced curing. J. Bacteriol. 154:1-9.
7. Hayes, F., C. Daly, and G. F. Fitzgerald. 1990. Identification of the minimal replicon of Lactococcus lactis subsp. lactis UC317 plasmid pCI305. Appl. Environ. Microbiol. 56:202-219.
8. He, B., A. Shiau, K. Y. Choi, H. Zalkin, and J. M. Smith. 1990. Genes of the Escherichia coli pur regulon are negatively controlled by a repressor-operon interaction. J. Bacteriol. 172:4555-4562.
9. Holo, H., and I. F. Nes. 1995. Transformation of Lactococcus by electroporation. Methods Mol. Biol. 47:195-199.
10. Israelsen, H., S. M. Madsen, A. Vrang, E. B. Hansen, and E. Johansen. 1995. Cloning and partial characterization of regulated promoters from Lactococcus lactis TnP17-lacZ integrants with the new promoter probe vector, pAK80. Appl. Environ. Microbiol. 61:2540-2547.
11. Jensen, P. R., and K. Hammer. 1993. Minimal requirements for exponential growth of Lactococcus lactis. Appl. Environ. Microbiol. 59:4363-4366.
12. Kilstrup, M., and J. Martinussen. 1998. A transcriptional activator, homologous to the Bacillus subtilis PurR repressor, is required for expression of purine biosynthetic genes in Lactococcus lactis. J. Bacteriol. 180:3907-3916.
13. Kilstrup, M., L. M. Meng, J. Neuhard, and P. Nygaard. 1989. Genetic evidence for a repressor of synthesis of cytosine deaminase and purine biosynthetic enzymes in Escherichia coli. J. Bacteriol. 171:2124-2127.
14. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265-275.
15. Martinussen, J., P. S. Andersen, and K. Hammer. 1994. Nucleotide metabolism in Lactococcus lactis: salvage pathways of exogenous pyrimidines. J. Bacteriol. 176:1514-1516.
16. Meng, L. M., M. Kilstrup, and P. Nygaard. 1990. Autoregulation of PurR repressor synthesis and involvement of purR in the regulation of purB, purC, purL, purMN and guaBA expression in Escherichia coli. Eur. J. Biochem. 187:373-379.
17. Meng, L. M., and P. Nygaard. 1990. Identification of hypoxanthine and guanine as the corepressors for the purine regulon genes of Escherichia coli. Mol. Microbiol. 4:2187-2192.
18. Miller, J. H. 1972. Assay of β-galactosidase, p. 352-355. In J. H. Miller (ed.), Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y.
19. Neuhard, J., and P. Nygaard. 1987. Purines and pyrimidines, p. 445-473. In F. C. Neidhardt, J. L. Ingraham, K. B. Low, B. Magasanik, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella typhimurium cellular and molecular biology. American Society for Microbiology, Wash-ington, D.C.
20. Nilsson, D., and M. Kilstrup. Cloning and expression of the Lactococcus lactis purDEK genes, required for growth on milk. Submitted for publication.
21. Nilsson, D., and A. A. Lauridsen. 1992. Isolation of purine auxotrophic mutants of Lactococcus lactis and characterization of the gene hpt encoding hypoxanthine guanine phosphoribosyltransfcrase. Mol. Gen. Genet. 235: 359-364.
22. Nygaard, P. 1993. Purine and pyrimidine salvage pathways, p. 359-378. In A. L. Sonenshein, J. A. Hoch, and R. Losick (ed.), Bacillus subtilis and other gram-positive bacteria: biochemistry, physiology, and molecular genetics, 1st ed. American Society for Microbiology, Washington, D.C.
23. Rolfes, R. J., and H. Zalkin. 1988. Escherichia coli gene purR encoding a repressor protein for purine nucleotide synthesis. J. Biol. Chem. 263:19653-19661.
24. Rolfes, R. J., and H. Zalkin. 1990. Purification of the Escherichia coli purine regulon repressor and identification of corepressors. J. Bacteriol. 172:5637-5642.
25. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
26. Saxild, H. H., and P. Nygaard. 1991. Regulation of levels of purine biosynthetic enzymes in Bacillus subtilis: effects of changing nucleotide pools. J. Gen. Microbiol. 137:2387-2394.
27. Schell, M. A. 1993. Molecular biology of the LysR family of transcriptional activators. Annu. Rev. Microbiol. 47:597-626.
28. Schumacher, M. A., K. Y. Choi, H. Zalkin, and R. G. Brennan. 1994. Crystal structure of Lad member, PurR, bound to DNA: minor groove binding by alpha helices. Science 266:763-770.
29. Shin, B. S., A. Stein, and H. Zalkin. 1997. Interaction of Bacillus subtilis purine repressor with DNA. J. Bacteriol. 179:7394-7402.
30. Teuber, M. 1995. The genus Lactococcus, p. 173-234. In B. J. B. Wood and W. H. Holzapfel (ed.), The genera of lactic acid bacteria. Blackie Academic Press, Glasgow, Scotland.
31. Tiedeman, A. A., D. J. DeMarini, J. Parker, and J. M. Smith. 1990 DNA sequence of the purC gene encoding 5′-phosphoribosyl-5-aminoimidazole-4-N-succinocarboxamide synthetase and organization of the dapA-purC region of Escherichia coli K-12. J. Bacteriol. 172:6035-6041.
32. Vogel, U., M. Sørensen, S. Pedersen, K. F. Jensen, and M. Kilstrup. 1992. Decreasing transcription elongation rate in Escherichia coli exposed to amino acid starvation. Mol. Microbiol. 6:2191-2200.
33. Weng, M., P. L. Nagy, and H. Zalkin. 1995. Identification of the Bacillus subtilis pur operon repressor. Proc. Natl. Acad. Sci. USA 92:7455-7459.
34. Zalkin, H. 1993. De novo purine nucleotide synthesis, p. 335-339. In A. L. Sonenshein, J. A. Hoch, and R. Losick (ed.), Bacillus subtilis and other gram-positive bacteria: biochemistry, physiology, and molecular genetics, 1st ed. American Society for Microbiology, Washington, D.C.
35. Zalkin, H., and J. E. Dixon. 1992. De novo purine nucleotide biosynthesis. Prog. Nucleic Acid Res. Mol. Biol., p. 258-287.
36. Zalkin, H., and P. Nygaard. 1996. Biosynthesis of purine nucleotides, p. 561-579. In F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Retznikoff, M. Riley, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed. American Society for Microbiology, Washington, D.C.