An optimized reverse transcription-polymerase chain reaction procedure for the amplification of low-copy Cyp2a3 mRNA in rat esophagus

Analytical Biochemistry

Volumn 319, Issue 2, 2003, Pages 323-326

  Ferreira, Anna B Robottom ^a   Pinto, Luís F Ribeiro ^a   Albano, Rodolpho M ^a  

^a STATE UNIVERSITY OF RIO DE JANEIRO   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CHEMISTRY;

REVERSE TRANSCRIPTION-POLYMERASE CHAIN REACTION;

POLYMERASE CHAIN REACTION;

COMPLEMENTARY DNA; CYTOCHROME P450 2A; CYTOCHROME P450 2A3; MESSENGER RNA; TAQ POLYMERASE; UNCLASSIFIED DRUG;

AGAR GEL ELECTROPHORESIS; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DNA MODIFICATION; ENZYME DENATURATION; ESOPHAGUS MUCOSA; GENE AMPLIFICATION; GENE EXPRESSION; GENE FREQUENCY; GENE IDENTIFICATION; GENETIC ANALYSIS; MALE; NONHUMAN; NOSE MUCOSA; PRIORITY JOURNAL; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION;

ANIMALIA;

EID: 0037829337     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(03)00293-8     Document Type: Article

References (19)

1. Carninci P., Nishiyama Y., Westover A., Itoh M., Nagaoka S., Sasaki N., Okazaki Y., Muramatsu M., Hayashizaki Y. Thermostabilization and thermoactivation of thermolabile enzymes by trehalose and its application for the synthesis of full length cDNA. Proc. Natl. Acad. Sci. USA. 95:1998;520-524.
2. Spiess A.N., Ivell R. A highly efficient method for long-chain cDNA synthesis using trehalose and betaine. Anal. Biochem. 301:2002;168-174.
3. Chou Q., Russell M., Birch D.E., Raymond J., Bloch W. Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications. Nucleic Acids Res. 20:1992;1717-1723.
4. Hebert B., Bergeron J., Potworowski E.F., Tijssen P. Increased PCR sensitivity by using paraffin wax as a reaction mix overlay. Mol. Cell. Probes. 7:1993;249-252.
5. Kellogg D.E., Rybalkin I., Chen S., Mukhamedova N., Vlasik T., Siebert P.D., Chenchik A. TaqStart Antibody: "hot start" PCR facilitated by a neutralizing monoclonal antibody directed against Taq DNA polymerase. BioTechniques. 16:1994;1134-1137.
6. Moretti T., Koons B., Budowle B. Enhancement of PCR amplification yield and specificity using AmpliTaq Gold DNA polymerase. BioTechniques. 25:1998;716-722.
7. Ailenberg M., Silverman M. Controlled hot start and improved specificity in carrying out PCR utilizing touch-up and loop incorporated primers (TULIPS). BioTechniques. 29:2000;1018-1024.
8. Kaboev O.K., Luchkina L.A., Tret'iakov A.N., Bahrmand A.R. PCR hot start using primers with the structure of molecular beacons (hairpin-like structure). Nucleic Acids Res. 28:2000;E94.
9. Dang C., Jayasena S.D. Oligonucleotide inhibitors of Taq DNA polymerase facilitate detection of low copy number targets by PCR. J. Mol. Biol. 264:1996;268-278.
10. Lin Y., Jayasena S.D. Inhibition of multiple thermostable DNA polymerases by a heterodimeric aptamer. J. Mol. Biol. 271:1997;100-111.
11. Weissensteiner T., Lanchbury J.S. Strategy for controlling preferential amplification and avoiding false negatives in PCR typing. Biotechniques. 21:1996;1102-1108.
12. Varadaraj K., Skinner D.M. Denaturants or cosolvents improve the specificity of PCR amplification of a G. + C-rich DNA using genetically engineered DNA polymerases Gene. 140:1994;1-5.
13. Kovarova M., Draber P. New specificity and yield enhancer of polymerase chain reactions. Nucleic Acids Res. 28:2000;E70.
14. Gopalakrishnan R., Morse M.A., Lu J., Weghorst C.M., Sabourin C.L.K., Stoner G.D., Murphy S.E. Expression of cytochrome P450 2A3 in rat oesophagus: relevance to N-nitrosobenzylmethylamine. Carcinogenesis. 20:1999;885-891.
15. Ribeiro Pinto L.F., Moraes E., Albano R.M., Silva C.C., Godoy W., Glosovic T., Lang M.A. The rat oesophageal cytochrome P450 (CYP) monooxygenase system: comparison to the liver and relevance to N-nitrosodiethylamine carcinogenesis. Carcinogenesis. 22:2001;1877-1883.
16. Lijinsky W. Chemistry and Biology of N-nitroso Compounds. 1992;Cambridge University Press, Cambridge, UK.
17. Bereziat J.C., Raffalli F., Schmezer P., Frei E., Geneste O., Lang M.A. Cytochrome P450 2A of nasal epithelium: regulation and role in carcinogen metabolism. Mol. Carcinogen. 14:1995;130-139.
18. Kimura S., Kozak C.A., Gonzalez F.J. Identification of a novel P450 expressed in rat lung: cDNA cloning and sequence, chromosome mapping, and induction by 3-methylcholanthrene. Biochemistry. 28:1989;3798-3803.
19. Hellmond H., Magnusson M., Huikko M.P., Rylander T., Gustafsson J.A., Warner M. Identification of CYP2A3 as a major cytochrome P450 enzyme in the female peripubertal rat breast. Mol. Pharmacol. 53:1998;475-482.