Inhibition of multiple thermostable DNA polymerases by a heterodimeric aptamer

Journal of Molecular Biology

Volumn 271, Issue 1, 1997, Pages 100-111

  Lin, Yun ^a   Jayasena, Sumedha D ^a  

^a GILEAD SCIENCES   (United States)

Author keywords

'hot start' PCR; Affinity selection; Stoffel fragment; Taq DNA polymerase; Tth DNA polymerase

Indexed keywords

DNA POLYMERASE; SINGLE STRANDED DNA;

ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME INHIBITION; GENE AMPLIFICATION; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; THERMUS AQUATICUS; THERMUS THERMOPHILUS;

THERMUS; THERMUS AQUATICUS; THERMUS THERMOPHILUS;

EID: 0031559579     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1997.1165     Document Type: Article

References (30)

1. Barnes W. M. PCR amplification of up to 35-kb DNA with high fidelity and high yield from λ bacteriophage templates. Proc. Natl Acad. Sci. USA. 91:1994;2216-2220.
2. Birch D. E., Kolmodlin L., Wong J., Zangenberg G. A., Zoccoli M. A. Simplified hot start PCR. Nature. 381:1996;445-446.
3. Bock L. C., Griffin L. C., Latham J. A., Vermaas E. H., Toole J. J. Selection of single-stranded DNA molecules that bind and inhibit human thrombin. Nature. 355:1992;564-566.
4. Chen H., Gold L. Selection of high-affinity RNA ligands to reverse transcriptase: inhibition of cDNA synthesis and RNase H activity. Biochemistry. 33:1994;8746-8756.
5. Chou Q., Russel M., Birch D. E., Raymond J., Bloch W. Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications. Nucl. Acids Res. 20:1992;1717-1723.
6. Connell G. J., Illangasekare M., Yarus M. Three small ribonucleotides with specific arginine sites. Biochemistry. 32:1993;5497-5502.
7. Dang C., Jayasena S. D. DNA inhibitors ofTaq. J. Mol. Biol. 264:1996;268-278.
8. D'Aquila R. T., Bechtel L. J., Videler J. A., Eron J. J., Gorczyca P., Kaplan J. C. Maximizing sensitivity and specificity of PCR by pre-amplification heating. Nucl. Acids Res. 19:1991;3749.
9. Davis K. A., Abrams B., Lin Y., Jayasena S. D. Use of a high affinity DNA ligand in flow cytometry. Nucl. Acids Res. 24:1996;702-706.
10. Deng S.-J., MacKenzie C. R., Hirama T., Brousseau R., Lowary T. L., Young N. M., Bundle D. R., Narang S. A. Basis for selection of improved carbohydrate-binding single-chain antibodies from synthetic gene libraries. Proc. Natl Acad. Sci. USA. 92:1995;4992-4996.
11. Ellington A. D. Aptamers achieve the desired recognition. Curr. Biol. 4:1994;427-429.
12. Famulok M., Szostak J. W. In vitro. Angew. Chem. Int. Ed. Engl. 31:1992;979-988.
13. Gold L., Polisky B., Uhlenbeck O., Yarus M. Diversity of oligonucleotide functions. Annu. Rev. Biochem. 64:1995;763-797.
14. Hebert B., Bergeron J., Potworowski E. F., Tijssen P. Increased PCR sensitivity by using paraffin wax as a reaction mix overlay. Mol Cell. Probes. 7:1993;249-252.
15. Horton R. M., Hoppe B. L., Conti-Tronconi B. M. Ampligrease: "hot start" PCR using petroleum jelly. BioTechniques. 16:1994;42-43.
16. Jellinek D., Green L. S., Bell C., Janjic N. Inhibition of receptor binding by high-affinity RNA ligands to vascular endothelial growth factor. Biochemistry. 33:1994;10450-10456.
17. Joyce G. F. In vitro. Curr. Opin. Struct. Biol. 4:1994;331-336.
18. ™Taq. BioTechniques. 16:1994;1134-1137.
19. Kiessling L. L., Pohl N. L. Strength in numbers: non-natural polyvalent carbohydrate derivatives. Curr. Biol. 3:1996;71-77.
20. Lawyer C. F., Stoffel S., Saiki R. K., Chang S.-Y., Landre P. A., Abramson R. D., Gelfand D. H. High-level expression, purification, and enzymatic characterization of full-lengthThermus aquaticus. PCR Methods Applic. 2:1993;275-287.
21. Li H., Cui X., Arnheim N. Direct electrophoretic detection of the allelic state of single DNA molecules in human sperm by using the polymerase chain reaction. Proc. Natl Acad. Sci. USA. 87:1990;4580-4584.
22. Lin Y., Padmapriya A., Morden K. M., Jayasena S. D. Peptide conjugation to anin vitro. Proc. Natl Acad. Sci. USA. 92:1995;11044-11048.
23. Myers T. W., Gelfand D. H. Reverse transcription and DNA amplification by aThermus thermophilus. Biochemistry. 30:1991;7661-7666.
24. Sassanfar M., Szostak J. W. An RNA motif that binds ATP. Nature. 364:1993;550-553.
25. Sobral B. W., Honeycutt R. J. High output genetic mapping of polyploids using PCR generated markers. Theoret. Appl. Genet. 86:1993;105-112.
26. Szostak J. W. In vitro. Trends Biochem. Sci. 17:1992;89-93.
27. Tada M., Omata M., Kawai S., Saisho H., Ohto M., Saiki R. K., Sninsky J. J. Detection ofras. Cancer Res. 53:1993;2472-2474.
28. Tuerk C., Eddy S., Parma D., Gold L. Autogenous translational operator recognized by bacteriophage T4 DNA polymerase. J. Mol. Biol. 213:1990;749-761.
29. Tuerk C., MacDougal S., Gold L. RNA pseudoknots that inhibit HIV-1 reverse transcriptase. Proc. Natl Acad. Sci. USA. 89:1992;6988-6992.
30. Wiegand T. W., Williams P. B., Dreskin S. C., Jouvin M.-H., Kinet J.-P., Tasset D. High-affinity oligonucleotide ligands to human IgE inhibit binding to Fce receptor I. J. Immunol. 157:1996;231-238.