Measurement of DNA breaks and oxidative damage in polymorphonuclear and mononuclear white blood cells: A novel approach using the comet assay

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 538, Issue 1-2, 2003, Pages 71-80

  Giovannelli, Lisa ^a   Pitozzi, Vanessa ^a   Riolo, Silvia ^a   Dolara, Piero ^a  

^a UNIVERSITY OF FLORENCE   (Italy)

Author keywords

DNA damage; DNA oxidation; Granulocytes; Lymphocytes

Indexed keywords

DNA;

ADULT; ANALYTIC METHOD; ARTICLE; CELL ASSAY; CELL DAMAGE; CELL ISOLATION; CELL TYPE; COMET ASSAY; DNA DAMAGE; DNA DETERMINATION; DNA STRAND BREAKAGE; FEMALE; GENETIC ANALYSIS; HUMAN; HUMAN CELL; LEUKOCYTE; MALE; MONONUCLEAR CELL; NORMAL HUMAN; POLYMORPHONUCLEAR CELL; PRIORITY JOURNAL;

EID: 0037756724     PISSN: 13835718     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1383-5718(03)00090-1     Document Type: Article

References (32)

1. Ames B. Dietary carcinogens and anticarcinogens. Oxygen radicals and degenerative diseases. Science. 221:1983;1256-1264.
2. European Standards Committee on Oxidative DNA Damage (ESCODD), Comparison of different methods of measuring 8-oxoguanine as a marker of oxidative DNA damage, Free Radic. Res. 32 (2000) 333-341.
3. ESCODD, Inter-laboratory validation of procedures for measuring 8-oxo-7,8-dihydroguanine/8-oxo-7,8-dihydro-2′-deoxyguanosine in DNA, Free Radic. Res. 36 (2002) 239-245.
4. Dandona P., Thusu K., Cook S., Snyder B., Makowski J., Armstrong D., Nicotera T. Oxidative damage to DNA in diabetes mellitus. Lancet. 347:1996;444-445.
5. Collins A.R., Raslova K., Somorovska M., Petrovska H., Ondrusova A., Vohnout B., Fabry R., Dusinska M. DNA damage in diabetes: correlation with a clinical marker. Free Radic. Biol. Med. 25:1998;373-377.
6. De Flora S., Izzotti A., Walsh D., Degan P., Petrilli G.L., Lewtas J. Molecular epidemiology of atherosclerosis. FASEB J. 11:1997;1021-1031.
7. Collins A.R., Gedik C.M., Olmedilla B., Southon S., Bellizzi M. Oxidative DNA damage measured in human lymphocytes: large differences between sexes and between countries, and correlations with heart disease mortality rates. FASEB J. 12:1998;1397-1400.
8. Raslova K., Dobiasova M., Nagyova A., Fabry R., Rauchova H., Dusinska M. Ciprofibrate treatment in patients with atherogenic lipoprotein phenotype: effects on HDL quality. Eur. J. Clin. Pharmacol. 54:1998;697-699.
9. Bashir S., Harris G., Denman M.A., Blake D.R., Winyard P.G. Oxidative DNA damage and cellular sensitivity to oxidative stress in human autoimmune diseases. Ann. Rheum. Dis. 52:1993;659-666.
10. Moller P., Knudsen L.E., Loft S., Wallin H. The comet assay as a rapid test in biomonitoring occupational exposure to DNA damaging agents and effect of confounding factors. Cancer Epidemiol. Biomarkers Prev. 9:2000;1005-1015.
11. Collins A.R. Oxidative DNA damage, antioxidants, and cancer. Bioessays. 21:1999;238-246.
12. Singh N.P., McCoy M.T., Tice R.R., Scheider E.L. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell Res. 175:1988;184-191.
13. Collins A.R., Duthie S.J., Dobson V.L. Direct enzymic detection of endogenous oxidative base damage in human lymphocyte DNA. Carcinogenesis. 14:1993;1733-1735.
14. Giovannelli L., Saieva C., Masala G., Testa G., Salvini S., Pitozzi V., Riboli E., Dolara P., Palli D. Nutritional and lifestyle determinants of DNA oxidative damage: a study in a Mediterranean population. Carcinogenesis. 23:2002;1483-1489.
15. 2-production by human neutrophils. The preparation and assay of NADPH oxidase-containing particles from human neutrophils. Methods Enzymol. 105:1984;358-365.
16. R.A. Floyd, The role of 8-hydroxyguanine in carcinogenesis, Carcinogenesis 11 (1090) 1447-1450.
17. Dizdaroglu M. Chemical determination of free-radical induced damage to DNA. Free Radic. Biol. Med. 10:1991;225-242.
18. Collins A.R., Dusinska M., Gedik C.M., Stetina R. Oxidative damage to DNA: do we have a reliable biomarker? Environ. Health Perspect. 104(Suppl. 3):1996;465-469.
19. Vijayalaxmi, G.H. Strauss, R.R. Tice, An analysis of gamma-ray-induced DNA damage in human blood leukocytes, lymphocytes and granulocytes, Mutat. Res. 292 (1993) 123-128.
20. Lankinen M.H., Vilpo L.M., Vilpo J.A. UV- and gamma-irradiation-induced DNA single-strand breaks and their repair in human blood granulocytes and lymphocytes. Mutat. Res. 352:1996;31-38.
21. Nakajima M., Takeuchi T., Takeshita T., Morimoto K. 8-Hydroxydeoxyguanosine in human leukocyte DNA and daily health practice factors: effects of individual alcohol sensitivity. Environ. Health Perspect. 104:1996;1336-1338.
22. Yasunari K., Maeda K., Nakamura M., Yoshikawa J. Oxidative stress in leukocytes is a possible link between blood pressure, blood glucose, and C-reacting protein. Hypertension. 39:2002;777-780.
23. Hannon-Fletcher M.P.A., O'Kane M.J., Moles K.W., Weatherup C., Barnett C.R., Barnett Y.A. Levels of peripheral blood cell DNA damage in insulin dependent diabetes mellitus human subjects. Mutat. Res. 460:2000;53-60.
24. Mustonen R., Hemminki K. 7-Methylguanine levels in DNA of smokers' and non-smokers' total white blood cells. Carcinogenesis. 13:1992;1951-1955.
25. Andreoli C., Rossi S., Leopardi P., Crebelli R. DNA damage by hydroquinone in human white blood cells: analysis by alkaline single-cell gel electrophoresis. Mutat. Res. 438:1999;37-45.
26. Pirmohamed M., Williams D., Tingle M.D., Barry M., Khoo S.H., O'Mahony C., Wilkins E.G., Breckenridge A.M., Park B.K. Intracellular glutathione in the peripheral blood cells of HIV-infected patients: failure to show a deficiency. AIDS. 10:1996;501-507.
27. Musallam L., Ethier C., Haddad P.S., Denizeau F., Bilodeau M. Resistance to Fas-induced apoptosis in hepatocytes: role of GSH depletion by cell isolation and culture. Am. J. Physiol. Gastrointest. Liver. Physiol. 283:2002;G709-G718.
28. Pietarinen-Runtti P., Lakari E., Raivio K.O., Kinnula V.L. Expression of antioxidant enzymes in human inflammatory cells. Am. J. Physiol. Cell Physiol. 278:2000;C118-C125.
29. Garaj-Vrhovac V., Zeljezic D. Cytogenetic monitoring of croatian population occupationally exposed to a complex mixture of pesticides. Toxicology. 165:2001;153-162.
30. Dincer Y., Akcay T., Alademir Z., Ilkova H. Assessment of DNA base oxidation and glutathione level in patients with type 2 diabetes. Mutat. Res. 505:2002;75-81.
31. Diaz-Llera S., Gonzalez-Hernandez Y., Prieto-Gonzalez E.A., Azoy A. Genotoxic effect of ozone in human peripheral blood leukocytes. Mutat. Res. 517:2002;13-20.
32. Migliore L., Petrozzi L., Lucetti C., Gambaccini G., Bernardini S., Scarpato R., Trippi F., Barale R., Frenzilli G., Rodilla V., Bonuccelli U. Oxidative damage and cytogenetic analysis in leukocytes of Parkinson's disease patients. Neurology. 58:2002;1809-1815.