A fluorescence study of the structure and accessibility of plasmid DNA condensed with cationic gene delivery vehicles

Journal of Pharmaceutical Sciences

Volumn 92, Issue 6, 2003, Pages 1272-1285

  Wiethoff, Christopher M ^a,c   Gill, Michelle L ^a,d   Koe, Gary S ^b   Koe, Janet G ^b   Middaugh, C Russell ^a  

^a UNIVERSITY OF KANSAS   (United States)

^b Urigen Pharmaceuticals Inc   (United States)

^c SCRIPPS RESEARCH INSTITUTE   (United States)

^d YALE UNIVERSITY   (United States)

Author keywords

Cationic lipid; DNA; Fluorescence; Hydration; Intercalation; Minor groove; Polymer

Indexed keywords

1,2 DIOLEOYL 3 TRIMETHYLAMMONIOPROPANE; 4 [5 (4 METHYL 1 PIPERAZINYL)[2,5' BI 1H BENZIMIDAZOL] 2' YL]PHENOL; 4',6 DIAMIDINO 2 PHENYLINDOLE; AMMONIUM DERIVATIVE; CATION; CHOLESTEROL; DIMETHYLDIOCTADECYLAMMONIUM BROMIDE; DIOLEOYLPHOSPHATIDYLETHANOLAMINE; DRUG VEHICLE; ETHANOLAMINE DERIVATIVE; ETHIDIUM BROMIDE; FLUORESCENT DYE; GLYCEROPHOSPHOETHANOLAMINE; INDOLE DERIVATIVE; PLASMID DNA; POLYCATION; POLYETHYLENEIMINE; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; DNA BINDING; DNA STRUCTURE; DRUG DELIVERY SYSTEM; FLUORESCENCE; GENE DELIVERY SYSTEM; INTERCALATION COMPLEX; POLYMERIZATION; SPECTROSCOPY; STRUCTURE ACTIVITY RELATION;

EID: 0037607609     PISSN: 00223549     EISSN: None     Source Type: Journal    
DOI: 10.1002/jps.10391     Document Type: Article

References (47)

1. Wiethoff CM, Middaugh CR. 2001. Light scattering techniques for characterization of synthetic gene therapy vectors. Methods Mol Med 65:349-376.
2. Zuidam NJ, Barenholz Y, Minsky A. 1999. Chiral DNA packaging in DNA-cationic liposome assemblies. FEBS Lett 457(3):419-422.
3. Choosakoonkriang S, Wiethoff CM, Anchordoquy TJ, Koe GS, Smith JG, Middaugh CR. 2001. Infrared spectroscopic characterization of the interaction of cationic lipids with plasmid DNA. J Biol Chem 276(11):8037-8043.
4. Eastman SJ, Siegel C, Tousignant J, Smith AE, Cheng SH, Scheule RK. 1997. Biophysical characterization of cationic lipid: DNA complexes. Biochim Biophys Acta 1325(1):41-62.
5. Zuidam NJ, Barenholz Y. 1998. Electrostatic and structural properties of complexes involving plasmid DNA and cationic lipids commonly used for gene delivery. Biochim Biophys Acta 1368(1):115-128.
6. Xu Y, Hui SW, Frederik P, Szoka FC Jr. 1999. Physicochemical characterization and purification of cationic lipoplexes. Biophys J 77(1):341-353.
7. Smith JG, Wedeking T, Vernachio JH, Way H, Niven RW. 1998. Characterization and in vivo testing of a heterogeneous cationic lipid-DNA formulation. Pharm Res 15(9):1356-1363.
8. Huebner S, Battersby BJ, Grimm R, Cevc G. 1999. Lipid-DNA complex formation: Reorganization and rupture of lipid vesicles in the presence of DNA as observed by cryoelectron microscopy. Biophys J 76(6):3158-3166.
9. Tarahovsky YS, Rakhmanova VA, Epand RM, MacDonald RC. 2002. High temperature stabilization of DNA in complexes with cationic lipids. Biophys J 82(1 Pt 1):264-273.
10. Kennedy MT, Pozharski EV, Rakhmanova VA, MacDonald RC. 2000. Factors governing the assembly of cationic phospholipid-DNA complexes. Biophys J 78(3):1620-1633.
11. Birchall JC, Kellaway IW, Mills SN. 1999. Physicochemical characterisation and transfection efficiency of lipid-based gene delivery complexes. Int J Pharm 183(2):195-207.
12. Koltover I, Salditt T, Safinya CR. 1999. Phase diagram, stability, and overcharging of lamellar cationic lipid-DNA self-assembled complexes. Biophys J 77(2):915-924.
13. Radler JO, Koltover I, Salditt T, Safinya CR. 1997. Structure of DNA-cationic liposome complexes: DNA intercalation in multilamellar membranes in distinct interhelical packing regimes. Science 275(5301):810-814.
14. Koltover I, Salditt T, Radler JO, Safinya CR. 1998. An inverted hexagonal phase of cationic liposome-DNA complexes related to DNA release and delivery. Science 281(5373):78-81.
15. Golan R, Pietrasanta LI, Hsieh W, Hansma HG. 1999. DNA toroids: Stages in condensation. Biochemistry 38(42):14069-14076.
16. Dunlap DD, Maggi A, Soria MR, Monaco L. 1997. Nanoscopic structure of DNA condensed for gene delivery. Nucleic Acids Res 25(15):3095-3101.
17. Bhattacharya S, Mandal SS. 1998. Evidence of interlipidic ion-pairing in anion-induced DNA release from cationic amphiphile-DNA complexes: Mechanistic implications in transfection. Biochemistry 37(21):7764-7777.
18. Zuidam NJ, Hirsch-Lerner D, Margulies S, Barenholz Y. 1999. Lamellarity of cationic liposomes and mode of preparation of lipoplexes affect transfection efficiency. Biochim Biophys Acta 1419(2):207-220.
19. Akao T, Fukumoto T, Ihara H, Ito A. 1996. Conformational change in DNA induced by cationic bilayer membranes. FEBS Lett 391(1-2):215-218.
20. Damaschun H, Damaschun G, Becker M, Buder E, Misselwitz R, Zirwer D. 1978. [Secondary structure of condensed DNA. Wide-angle, small-angle x-ray scattering and circular dichroism]. Acta Biol Med Ger 37(4):569-576.
21. Ringquist S, Shinn R, Hanlon S. 1989. Linking number anomalies in DNA under conditions close to condensation. Biochemistry 28(3):1076-1085.
22. Lobo BA, Rogers S, Choosakoonkriang S, Smith JG, Koe GS, Middaugh CR. 2002. Differential scanning calorimetric studies of the stability of plasmid DNA complexed with cationic lipids and polymers. J Pharm Sci 91(2):454-466.
23. Hirsch-Lerner D, Barenholz Y. 1999. Hydration of lipoplexes commonly used in gene delivery: Follow-up by laurdan fluorescence changes and quantification by differential scanning calorimetry. Biochim Biophys Acta 1461(1):47-57.
24. Gershon H, Ghirlando R, Guttman SB, Minsky A. 1993. Mode of formation and structural features of DNA-cationic liposome complexes used for transfection. Biochemistry 32(28):7143-7151.
25. Harvie P, Wong FM, Bally MB. 1998. Characterization of lipid DNA interactions. I. Destabilization of bound lipids and DNA dissociation. Biophys J 75(2):1040-1051.
26. McKenzie DL, Kwok KY, Rice KG. 2000. A potent new class of reductively activated peptide gene delivery agents. J Biol Chem 275(14):9970-9977.
27. Hard T, Nielsen PE, Norden B. 1988. Molecular flexibility of extended and compacted polynucleosomes: A steady-state fluorescence polarization study. Eur Biophys J 16(4):231-241.
28. Geall AJ, Blagbrough IS. 2000. Rapid and sensitive ethidium bromide fluorescence quenching assay of polyamine conjugate-DNA interactions for the analysis of lipoplex formation in gene therapy. J Pharm Biomed Anal 22(5):849-859.
29. Hard T, Kearns DR. 1990. Reduced DNA flexibility in complexes with a type II DNA binding protein. Biochemistry 29(4):959-965.
30. Ward LD. 1985. Measurement of ligand binding to proteins by fluorescence spectroscopy. Methods Enzymol 117:400-414.
31. Gaugain B, Barbet J, Capelle N, Roques BP, Le Pecq JB. 1978. DNA Bifunctional intercalators. II. Fluorescence properties and DNA binding interaction of an ethidium homodimer and an acridine ethidium heterodimer. Biochemistry 17(24):5078-5088.
32. Loontiens FG, McLaughlin LW, Diekmann S, Clegg RM. 1991. Binding of Hoechst 33258 and 4′,6′-diamidino-2-phenylindole to self-complementary decadeoxynucleotides with modified exocyclic base substituents. Biochemistry 30(1):182-189.
33. Breusegem SY, Clegg RM, Loontiens FG. 2002. Base-sequence specificity of Hoechst 33258 and DAPI binding to five (A/T)4 DNA sites with kinetic evidence for more than one high-affinity Hoechst 33258-AATT complex. J Mol Biol 315(5):1049-1061.
34. Lobo BA, Smith A, Smith JG, Koe GS, Middaugh CR. 2001. Calorimetric analysis of the interaction between cationic lipids and plasmid DNA using isothermal titration calorimetry. Arch Biochem Biophys 386(1):95-105.
35. Latt SA, Stetten G. 1976. Spectral studies on 33258 Hoechst and related bisbenzimidazole dyes useful for fluorescent detection of deoxyribonucleic acid synthesis. J Histochem Cytochem 24(1):24-33.
36. Eriksson S, Kim SK, Kubista M, Norden B. 1993. Binding of 4′,6-diamidino-2-phenylindole (DAPI) to AT regions of DNA: Evidence for an allosteric conformational change. Biochemistry 32(12):2987-2998.
37. Braun CS, Kueltzo LA, Middaugh CR. 2001. Ultraviolet absorption and circular dichroism spectroscopy of non-viral gene delivery complexes. Methods Mol Med 65:253-284.
38. Sailer BL, Nastasi AJ, Valdez JG, Steinkamp JA, Crissman HA. 1997. Differential effects of deuterium oxide on the fluorescence lifetimes and intensities of dyes with different modes of binding to DNA. J Histochem Cytochem 45(2):165-175.
39. Kubota Y, Murashige S, Fujisaki Y. 1986. Fluorescence studies of the interaction of DNA with Hoechst 33258. Nucleic Acids Symp Ser 17:219-222.
40. Chaires JB. 2001. Analysis and interpretation of ligand-DNA binding isotherms. Methods Enzymol 340:3-22.
41. Choosakoonkriang S, Wiethoff CM, Kueltzo LA, Middaugh CR. 2001. Characterization of synthetic gene delivery vehicles by infrared spectroscopy. Methods Mol Med 65:285-317.
42. Albert FG, Eckdahl TT, Fitzgerald DJ, Anderson JN. 1999. Heterogeneity in the actions of drugs that bind in the DNA minor groove. Biochemistry 38(31):10135-10146.
43. Bianchi F, Rousseaux-Prevost R, Bailly C, Rousseaux J. 1994. Interaction of human P1 and P2 protamines with DNA. Biochem Biophys Res Commun 201(3):1197-1204.
44. Bizzaro D, Manicardi GC, Bianchi PG, Bianchi U, Mariethoz E, Sakkas D. 1998. In situ competition between protamine and fluorochromes for sperm DNA. Mol Hum Reprod 4(2):127-132.
45. Manning GS, Ray J. 1998. Counterion condensation revisited. J Biomol Struct Dyn 16(2):461-476.
46. Hammermann M, Steinmaier C, Merlitz H, Kapp U, Waldeck W, Chirico G, Langowski J. 1997. Salt effects on the structure and internal dynamics of superhelical DNAs studied by light scattering and Brownian dynamics. Biophys J 73(5):2674-2687.
47. Langowski J. 1987. Salt effects on internal motions of superhelical and linear pUC8 DNA: Dynamic light scattering studies. Biophys Chem 27(3):263-271.