Crystal structures of the ribonuclease MC1 mutants N71T and N71S in complex with 5′-GMP: Structural basis for alterations in substrate specificity

Biochemistry

Volumn 42, Issue 18, 2003, Pages 5270-5278

  Numata, Tomoyuki ^a   Suzuki, Akio ^b   Kakuta, Yoshimitsu ^a,c   Kimura, Kazumi ^a   Yao, Min ^b   Tanaka, Isao ^b   Yoshida, Yuichiro ^a   Ueda, Tadashi ^a   Kimura, Makoto ^a  

^a KYUSHU UNIVERSITY   (Japan)

^b HOKKAIDO UNIVERSITY   (Japan)

^c RIKEN Harima Institute/SPring 8 ^*  (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; COMPLEXATION; CRYSTAL STRUCTURE; HYDROGEN BONDS; HYDROPHOBICITY; SUBSTRATES;

HYDROPHOBIC INTERACTIONS;

BIOCHEMISTRY;

GUANINE DERIVATIVE; GUANOSINE PHOSPHATE; RIBONUCLEASE; URACIL DERIVATIVE;

ARTICLE; CRYSTAL STRUCTURE; ENZYME SPECIFICITY; HYDROGEN BOND; HYDROPHOBICITY; IMAGE QUALITY; MUTATIONAL ANALYSIS; NONHUMAN; PRIORITY JOURNAL; STRUCTURE ANALYSIS; WILD TYPE;

5'-GUANYLIC ACID; CRYSTALLOGRAPHY, X-RAY; CUCURBITACEAE; GUANINE; HYDROGEN BONDING; MODELS, CHEMICAL; MUTAGENESIS, SITE-DIRECTED; MUTATION; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN FOLDING; RIBONUCLEASES; SEEDS; SUBSTRATE SPECIFICITY;

CUCURBITA; MOMORDICA CHARANTIA; NICOTIANA GLUTINOSA;

EID: 0037544149     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi034103g     Document Type: Article

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