Expression and mutational analysis of amino acid residues involved in catalytic activity in a ribonuclease MC1 from the seeds of bitter gourd

Bioscience, Biotechnology and Biochemistry

Volumn 64, Issue 3, 2000, Pages 603-605

  Numata, Tomoyuki ^a   Kashiba, Tohru ^a   Hino, Madoka ^a   Funatsu, Gunki ^a   Ishiguro, Masatune ^a   Yamasaki, Nobuyuki ^a   Kimura, Makoto ^a  

^a KYUSHU UNIVERSITY   (Japan)

Author keywords

Bitter gourd; Momordica charantia; RNase MC1; Site directed mutagenesis

Indexed keywords

AMINO ACID; HYBRID PROTEIN; RIBONUCLEASE; RIBONUCLEASE T2;

ARTICLE; CUCUMBER; ENZYME ACTIVE SITE; ENZYMOLOGY; GENE EXPRESSION; GENETICS; METABOLISM; PLANT SEED; SITE DIRECTED MUTAGENESIS;

AMINO ACIDS; CATALYTIC DOMAIN; CUCURBITACEAE; ENDORIBONUCLEASES; GENE EXPRESSION; MUTAGENESIS, SITE-DIRECTED; RECOMBINANT FUSION PROTEINS; SEEDS;

EID: 0034155191     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.64.603     Document Type: Article

References (14)

1. Ide, H., Kimura, M., Arai, M., and Funatsu, G., The complete amino acid sequence of ribonucléase from the seeds of bitter gourd (Momordica charantia). FEBS Lett., 284, 161-164 (1991).
2. Sato, K. and Egami, F., Studies on ribonucleases in takadiastase. J. Biochem., 44, 753-767 (1957).
3. Imazawa, M., Irie, M., and Ukita, T., Substrate specificity of ribonucléase from Aspergillus saitoi. J. Biochem., 64, 595-602 (1968).
4. Tomoyeda, M., Eto, Y., and Yoshino, T., Studies on ribonucléase produced by Rhizopus sp. I. Crystalli-zation and some properties of the ribonucléase. Arch. Biochem. Biophys., 131, 191-202 (1969).
5. Irie, M., Watanabe, H., Ohgi, K., Minami, Y., Yamada, H., and Funatsu, G., Base specificity of two plant seed ribonucléase from Momordica charantia and Luff a cylindrica. Biosci. Biotechnol. Biochem., 57, 497-498 (1993).
6. Irie, M., Structure-function relationships of acid ribonucléase: lysosomal, vacuolar, and periplasmic enzymes. Pharmacol. Ther., 8, 77-89 (1999).
7. Kurihara, H., Nonaka, T., Mitui, Y., Ohgi, K., Irie, M., Nakamura, K. T., The crystal structure of ribonucléase Rh from Rhizopus niveus at 2.0 Â resolution. J. Mol. Biol., 255, 310-320 (1996).
8. Nakagawa, A., Tanaka, I., Sakai, R., Nakashima, T., Funatsu, G., and Kimura, M., Crystal structure of a ribonucléase from the seeds of bitter gourd (Momordica charantia) at 1.75 Â resolution. Biochim. Biophys. Acta, 1433, 253-260 (1999).
9. Studier, F. W., Rosenberg, A. H. R., Dunn, J. J., and Dubendorff, J. W., Use of T7 RNA polymerase to direct expression of cloned genes. Methods En-zymol., 185, 60-89 (1990).
10. Abel, S. and Glund, K., Localization of RNA-degrading enzyme activity within vacuoles of cultured tomato cells. Physiol. Plant, 66, 79-86 (1986).
11. Deng, W. P. and Nickoloff, J. A., Site-directed mutagenesis of virtually any plasmid by eliminating a single site. Anal. Biochem., 200, 81-88 (1992).
12. Ohgi, K., Iwama, M., Tada, K., Takizawa, R., and Irie, M., Role of Lysl08 in the enzymatic activity of Rnase Rh from Rhizopus niveus. J. Biochem., 117, 27-33 (1993).
13. Ohgi, K., Horiuchi, H., Watanabe, H., Iwama, M., Takagi, M., and Irie, M., Role of Asp51 and Glul05 in the enzymatic activity of a ribonucléase from Rhizopus niveus. J. Biochem., 113, 219-224 (1993).
14. Ohgi, K., Horiuchi, H., Watanabe, H., Takagi, M., Yano, K., and Irie, M., Expression of RNase Rh from Rhizopus niveus in yeast and characterization of the secreted proteins. J. Biochem., 109, 776-785 (1991).