Exploration of critical parameters for transient retrovirus production

Journal of Biotechnology

Volumn 103, Issue 2, 2003, Pages 97-106

  Bajgelman, Marcio C ^a   Costanzi Strauss, Eugenia ^a   Strauss, Bryan E ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

pAdvantage; pCL; Titration; Transfection; Galactosidase

Indexed keywords

CELLS; CYTOLOGY; DNA; GENE TRANSFER; GENES; MEDICAL APPLICATIONS; PROTEINS; SYNTHESIS (CHEMICAL);

TRANSFECTION;

VIRUSES;

RETROVIRUS VECTOR; VIRUS PROTEIN;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DNA PACKAGING; EMBRYO; GENE TRANSFER; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; KINETICS; MOUSE; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN SYNTHESIS; RETROVIRUS; SUPERNATANT;

UNIDENTIFIED RETROVIRUS;

EID: 0037502299     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(03)00103-2     Document Type: Article

References (19)

1. Anderson W.F. Human gene therapy. Nature. 392:1998;25-30.
2. Costanzi-Strauss E., Strauss B.E., Naviaux R.K., Haas M. Restoration of growth arrest by p16INK4, p21WAF1, pRB, and p53 is dependent on the integrity of the endogenous cell-cycle control pathways in human glioblastoma cell lines. Exp. Cell Res. 238:1998;51-62.
3. Gerin P.A., Gilligan M.G., Searle P.F., Al-Rubeai M. Improved titers of retroviral vectors from the human FLYRD18 packaging cell line in serum- and protein-free medium. Hum. Gene Ther. 10:1999;1965-1974.
4. Gerin P.A., Searle P.F., Al-Rubeai M. Production of retroviral vectors for gene therapy with the human packaging cell line FLYRD18. Biotechnol. Prog. 15:1999;941-948.
5. Glimm H., Flugge K., Mobest D., Hofmann V.M., Postmus J., Henschler R., Lange W., Finke J., Kiem H.P., Schulz G., et al. Efficient serum-free retroviral gene transfer into primitive human hematopoietic progenitor cells by a defined, high-titer, nonconcentrated vector-containing medium. Hum. Gene Ther. 9:1998;771-778.
6. Kafri T. Lentivirus vectors: difficulties and hopes before clinical trials. Curr. Opin. Mol. Ther. 3:2001;316-326.
7. Kitajewski J., Schneider R.J., Safer B., Munemitsu S.M., Samuel C.E., Thimmappaya B., Shenk T. Adenovirus VAI RNA antagonizes the antiviral action of interferon by preventing activation of the interferon-induced eIF-2 alpha kinase. Cell. 45:1986;195-200.
8. Kitajewski J., Schneider R.J., Safer B., Shenk T. An adenovirus mutant unable to express VAI RNA displays different growth responses and sensitivity to interferon in various host cell lines. Mol. Cell. Biol. 6:1986;4493-4498.
9. Le Doux J.M., Morgan J.R., Snow R.G., Yarmush M.L. Proteoglycans secreted by packaging cell lines inhibit retrovirus infection. J. Virol. 70:1996;6468-6473.
10. Le Doux J.M., Morgan J.R., Yarmush M.L. Removal of proteoglycans increases efficiency of retroviral gene transfer. Biotechnol. Bioeng. 58:1998;23-34.
11. Le Doux J.M., Davis H.E., Morgan J.R., Yarmush M.L. Kinetics of retrovirus production and decay. Biotechnol. Bioeng. 63:1999;654-662.
12. McTaggart S., Al-Rubeai M. Effects of culture parameters on the production of retroviral vectors by a human packaging cell line. Biotechnol. Prog. 16:2000;859-865.
13. Mountain A. Gene therapy: the first decade. Trends Biotechnol. 18:2000;119-128.
14. Naviaux R.K., Costanzi E., Haas M., Verma I.M. The pCL vector system: rapid production of helper-free, high-titer, recombinant retroviruses. J. Virol. 70:1996;5701-5705.
15. O'Malley R.P., Mariano T.M., Siekierka J., Mathews M.B. A mechanism for the control of protein synthesis by adenovirus VA RNAI. Cell. 44:1986;391-400.
16. Podsakoff G.M. Lentiviral vectors approach the clinic but fall back: National Institutes of Health Recombinant DNA Advisory Committee review of a first clinical protocol for use of a lentiviral vector. Mol. Ther. 4:2001;282-283.
17. Soneoka Y., Cannon P.M., Ramsdale E.E., Griffiths J.C., Romano G., Kingsman S.M., Kingsman A.J. A transient three-plasmid expression system for the production of high titer retroviral vectors. Nucleic Acids Res. 23:1995;628-633.
18. Yang S., Delgado R., King S.R., Woffendin C., Barker C.S., Yang Z.Y., Xu L., Nolan G.P., Nabel G.J. Generation of retroviral vector for clinical studies using transient transfection. Hum. Gene Ther. 10:1999;123-132.
19. Yap M.W., Kingsman S.M., Kingsman A.J. Effects of stoichiometry of retroviral components on virus production. J. Gen. Virol. 81:2000;2195-2202.