Efficient serum-free retroviral gene transfer into primitive human hematopoietic progenitor cells by a defined, high-titer, nonconcentrated vector-containing medium

Human Gene Therapy

Volumn 9, Issue 6, 1998, Pages 771-778

  Glimm, Hanno ^a   Flügge, Kirsten ^a   Möbest, Dieter ^a   Hofmann, Veit Maria ^a   Postmus, Jan ^a   Henschler, Reinhard ^a   Lange, Winand ^a   Finke, Jürgen ^a   Kiem, Hans Peter ^b   Schulz, Gregor ^c   Rosenthal, Felicia ^c   Mertelsmann, Roland ^a   Von Kalle, Christof ^a  

^a UNIVERSITY OF FREIBURG   (Germany)

^b UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

^c Cellgenix GmbH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CD34 ANTIGEN; CD38 ANTIGEN; HLA DR ANTIGEN; INTERLEUKIN 3; VIRUS VECTOR;

ARTICLE; GENE TRANSFER; HEMATOPOIETIC STEM CELL; HUMAN; HUMAN CELL; RETROVIRUS;

BOVINAE; SIGANUS; UNIDENTIFIED RETROVIRUS;

EID: 17344371126     PISSN: 10430342     EISSN: None     Source Type: Journal    
DOI: 10.1089/hum.1998.9.6-771     Document Type: Article

References (10)

1. BERENSON, R.J., BENSINGER, W.I., HILL, R.S., ANDREWS, R.G., GARCIA, L.J., KALAMASZ, D.F., STILL, B.J., SPITZER, G., BUCKNER, C.D., BERNSTEIN, I.D., and THOMAS, E.D. (1991). Engraftment after infusion of CD34+ marrow cells in patients with breast cancer or neuroblastoma. Blood 77, 1717-1722.
2. BIENZLE, D., ABRAMS, O.A., KRUTH, S.A., ACKLAND, S.J., CARTER, R.F., DICK, J.E., JACOBS, R.M., KAMEL, R.S., and DUBE, I.D. (1994). Gene transfer into hematopoietic stem cells: Long-term maintenance of in vitro activated progenitors without marrow ablation. Proc. Natl. Acad. Sci. U.S.A. 91, 350-354.
3. BRENNER, M.K. (1993). Gene transfer into human hematopoietic progenitor cells: A review of current clinical protocols. J. Hematother. 2, 7-17.
4. BRENNER, M.K., RILL, D.R., HOLLADAY, M.S., HESLOP, H.E., MOEN, R.C., BUSCHLE, M., KRANCE, R.A., SANTANA, V.M., ANDERSON, W.F., and IHLE, J.N. (1993). Gene marking to determine whether autologous marrow infusion restores long-term haemopoiesis in cancer patients. Lancet 342, 1134-1137.
5. BRUGGER, W., HENSCHLER, R., HEIMFELD, S., BERENSON, R.J., MERTELSMANN, R., and KANZ, L. (1994). Positively selected autologous blood CD34+ cells and unseparated peripheral blood progenitor cells mediate identical hematopoietic engraftment after high-dose VP16, ifosfamide, carboplatin, and epirubicin. Blood 84, 1421-1426.
6. CONNEALLY, E., BARDY, P., EAVES, C.J., THOMAS, T., CHAPPEL, S., SHPALL, E.J., and HUMPHRIES, R.K. (1996). Rapid and efficient selection of human hematopoietic cells expressing murine heat-stable antigen as an indicator of retroviral-mediated gene transfer. Blood 87, 456-464.
7. DEISSEROTH, A.B., ZU, Z., CLAXTON, D., HANANIA, E.G., FU, S., ELLERSON, D., GOLDBERG, L., THOMAS, M., JANICEK, K., ANDERSON, W.F., HESTER, J., KORBLING, M., DURETT, A., MOEN, R., BERENSON, R., HEIMFELD, S., HAMER, J., CLAVERT, L., TIBBITS, P., TALPAZ, M., KANTARIJAN, H., CHAMPLIN, R., and REEDING, C. (1994). Genetic marking shows that Ph+ cells present in autologous transplants of chronic myel-ogenous leukemia (CML) contribute to relapse after autologous bone marrow in CML. Blood 83, 3068-3076.
8. DUNBAR, C.E. (1996). Gene transfer to hematopoietic stem cells -implications for gene therapy of human disease. Annu. Rev. Med. 47, 11-20 [Review].
9. VON KALLE, C., KIEM, H.P., GOEHLE, S., DAROVSKY, B., HEIMFELD, S., TOROK, S.B., STORE, R., and SCHUENING, F.G. (1994). Increased gene transfer into human hematopoietic progenitor cells by extended in vitro exposure to a pseudotyped retroviral vector. Blood 84, 2890-2897.
10. ZANDSTRA, P.W., CONNEALLY, E., PETZER, A.L., PIRET, J.M., and EAVES, C.J. (1997). Cytokine manipulation of primitive human hematopoietic cell self-renewal. Proc. Natl. Acad. Sci. U.S.A. 94, 4698-4703.