Codon optimization, genetic insulation, and an rtTA reporter improve performance of the tetracycline switch

Transgenic Research

Volumn 8, Issue 5, 1999, Pages 371-382

  Wells, Kevin D ^a   Foster, Juli A ^a   Moore, Karen ^a   Pursel, Vernon G ^a   Wall, Robert J ^a  

^a USDA ARS Bldg 200   (United States)

Author keywords

Codon bias; IRES; MAR; rtTA; TetR; tetR; Transgene switch; Transgenic

Indexed keywords

BIOASSAY; CODON; FLUORESCENCE; GENE INDUCTION; GENE REPRESSION; GENE SWITCHING; GREEN FLUORESCENT PROTEIN; PLASMID; POINT MUTATION; PROMOTER REGION; REPORTER GENE; RIBOSOME; TETRACYCLINE REPRESSOR PROTEIN; TISSUE CULTURE; TRANSGENE; WILD RELATIVE;

EID: 0033208332     PISSN: 09628819     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1008952302539     Document Type: Article

References (28)

1. Baim SB, Labow MA, Levine AJ and Shenk T (1991) A chimeric mammalian transactivator based on the lac repressor that is regulated by temperature and isopropyl beta-D-thiogalactopyranoside. Proc Natl Acad Sci USA 88: 5072-5076.
2. Boulikas T (1994) Transcription factor binding sites in the matrix attachment region (MAR) of the chicken alpha-globin gene. J Cell Biochem 55: 513-529.
3. Charron G, Julien JP and Bibor-Hardy V (1995) Neuron specificity of the neurofilament light promoter in transgenic mice requires the presence of DNA unwinding elements. J Biol Chem 270: 25739-25745.
4. Christopherson KS, Mark MR, Bajaj V and Godowski PJ (1992) Ecdysteroid-dependent regulation of genes in mammalian cells by a Drosophila ecdysone receptor and chimeric transactivators. Proc Natl Acad Sci USA 89: 6314-6318.
5. Delort JP and Capecchi MR (1996)TAXI/UAS: A molecular switch to control expression of genes in vivo. Hum Gene Ther 7: 809-820.
6. Evstafieva AG, Ugarova TY, Chernov BK and Shatsky IN (1991) A complex RNA sequence determines the internal initiation of encephalomyocarditis virus RNA translation (published erratum appears in Nucleic Adds Res (1991) 19: 1358). Nucleic Acids Res 19:665-671.
7. Furth PA, St Onge L, Boger H, Gruss P, Gossen M, Kistner A, Bujard H and Hennighausen L (1994) Temporal control of gene expression in transgenic mice by a tetracycline-responsive promoter. Proc Natl Acad Sci USA 91: 9302-9306.
8. Gossen M and Bujard H (1992) Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc Natl Acad Sci USA 89: 5547-5551.
9. Gossen M, Freundlieb S, Bender G, Muller G, Hillen W and Bujard H (1995) Transcriptional activation by tetracyclines in mammalian cells. Science 268: 1766-1769.
10. Haas J, Park EC and Seed B (1996) Codon usage limitation in the expression of HIV-1 envelope glycoprotein. Curr Biol 6: 315-324.
11. Hamada H (1986) Random isolation of gene activator elements from the human genome. Mol Cell Biol 6: 4185-4194.
12. Hu MC and Davidson N (1987) The inducihle lac operator-represser system is functional in mammalian cells. Cell 48: 555-566.
13. Kim CH, Oh Y and Lee TH (1997) Codon optimization for high-level expression of human erythropoietin (EPO) in mammalian cells. Gene 199: 293-301.
14. Kistner A, Gossen M, Zimmermann F, Jerecic J, Ullmer C, Lubbert H and Bujard H (1996) Doxycycline-mediated quantitative and tissue-specific control of gene expression in transgenic mice. Proc Natl Acad Sci USA 93: 10933-10938.
15. McKnight RA, Shamay A, Sankaran L, Wall RJ and Hennighausen L (1992) Matrix-attachment regions can impart position-independent regulation of a tissue-specific gene in transgenic mice. Proc Natl Acad Sci USA 89: 6943-6947.
16. McKnight RA, Spencer M, Wall RJ and Hennighausen L (1996) Severe position effects imposed on a 1 kb mouse whey acidic protein gene promoter are overcome by heterologous matrix attachment regions. Mol Reprod Dev 44: 179-184.
17. Mosser DD, Caron AW, Bourget L, Jolicoeur P and Massie B (1997) Use of a dicistronic expression cassette encoding the green fluorescent protein for the screening and selection of cells expressing inducible gene products. Biotechniques 22: 150-158.
18. Nakamura Y, Gojobori T, Ikemura T (1999) Codon usage tabulated from the international DNA sequence databases: its status 1999. Nucleic Acids Res 27: 292.
19. Neznanov N, Kohwi-Shigematsu T and Oshima RG (1996) Contrasting effects of the SATB1 core nuclear matrix attachment region and flanking sequences of the keratin 18 gene in transgenic mice. Mol Biol Cell 7: 541-552.
20. No D, Yao TP and Evans RM (1996) Ecdysone-inducible gene expression in mammalian cells and transgenic mice. Proc Natl Acad Sci USA 93: 3346-3351.
21. O'Brien K, Otto K and Rao RN (1997) Construction and characterization of a one-plasmid system for the controlled expression of genes in mammalian cells by tetracycline. Gene 184: 115-120.
22. Perlak FJ, Stone TB, Muskopf YM, Petersen LJ, Parker GB, McPherson SA, Wyman J, Love S, Reed G and Biever D (1993) Genetically improved potatoes: protection from damage by Colorado potato beetles. Plum Mol Biol 22: 313-321.
23. Phi-Van L, von Kries JP, Ostertag W and Stratling WH (1990) The chicken lysozyme 5′ matrix attachment region increases transcription from a heterologous promoter in heterologous cells and dampens position effects on the expression of transfected genes. Mol Cell Biol 10: 2302-2307.
24. Schultze N, Burki Y, Lang Y, Certa U and Bluethmann H (1996) Efficient control of gene expression by single step integration of the tetracycline system in transgenic mice. Nat Biotechnol 14: 499-503.
25. Stief A, Winter DM, Stratling WH and Sippel AE (1989) A nuclear DNA attachment element mediates elevated and position-independent gene activity. Nature 341: 343-345.
26. Sutton DW, Havstad PK and Kemp JD (1992) Synthetic cryIIIA gene from Bacillus thuringiensis improved for high expression in plants. Transgenic Res 1: 228-236.
27. von Kries JP, Phi-Van L, Diekmann S and Stratling WH (1990) A non-curved chicken lysozyme 5′ matrix attachment site is 3′ followed by a strongly curved DNA sequence. Nucleic Acids Res 18: 3881-3885.
28. Zhang G, Gurtu V and Kain SR (1996) An enhanced green fluorescent protein allows sensitive detection of gene transfer in mammalian cells. Biochcm Biophys Res Commun 227: 707-711.