Tandem immobilized metal-ion affinity chromatography/immunoaffinity purification of His-tagged proteins - Evaluation of two anti-His-tag monoclonal antibodies

Analytical Biochemistry

Volumn 259, Issue 1, 1998, Pages 54-61

  Müller, Kristian M ^a   Arndt, Katja M ^a   Bauer, Konrad ^b   Plückthun, Andreas ^a  

^a UNIVERSITY OF ZURICH   (Switzerland)

^b INSTITUTE OF EPIDEMIOLOGY   (Germany)

Author keywords

BIAcore; His tag; IMAC; Immunoaffinity chromatography

Indexed keywords

AFFINITY CHROMATOGRAPHY; AMINO ACIDS; ANTIGEN-ANTIBODY REACTIONS; CHELATION; DISSOCIATION; METAL IONS; METALS; MONOCLONAL ANTIBODIES; PURIFICATION; SURFACE PLASMON RESONANCE;

BIACORE; HIGH THROUGHPUT SCREENING; HIS-TAG; IMAC; IMMOBILIZED METAL ION AFFINITY CHROMATOGRAPHIES; IMMUNOAFFINITY CHROMATOGRAPHY; IMMUNOAFFINITY COLUMNS; SURFACE PLASMON RESONANCE BIOSENSOR;

ION CHROMATOGRAPHY;

EDETIC ACID; HISTIDINE; HISTIDINE TAGGED PROTEIN; IMIDAZOLE DERIVATIVE; MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY 3D5; MONOCLONAL ANTIBODY PENTAHIS; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ANTIBODY AFFINITY; ANTIBODY DETECTION; ANTIBODY STRUCTURE; ARTICLE; BINDING KINETICS; BIOSENSOR; DISSOCIATION CONSTANT; METAL BINDING; PRIORITY JOURNAL; PROTEIN PURIFICATION;

EID: 0042846629     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1998.2606     Document Type: Article

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