Threonines 2638/2647 in DNA-PK are essential for cellular resistance to ionizing radiation

Cancer Research

Volumn 63, Issue 6, 2003, Pages 1198-1201

  Soubeyrand, Sébastien ^a   Pope, Louise ^a   Pakuts, Benjamin ^a   Haché, Robert J G ^a,b  

^a UNIVERSITY OF OTTAWA   (Canada)

^b OTTAWA HOSPITAL RESEARCH INSTITUTE   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

DNA DEPENDENT PROTEIN KINASE; DOUBLE STRANDED DNA; THREONINE;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; AUTOPHOSPHORYLATION; CELL SURVIVAL; CONTROLLED STUDY; DNA STRAND BREAKAGE; ENZYME ACTIVATION; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; IN VITRO STUDY; IONIZING RADIATION; MOLECULAR CLONING; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RADIOSENSITIVITY;

AMINO ACID SEQUENCE; ANIMALS; BINDING SITES; CELL LINE; DNA DAMAGE; DNA REPAIR; DNA-ACTIVATED PROTEIN KINASE; DNA-BINDING PROTEINS; ENZYME ACTIVATION; MICE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PHOSPHORYLATION; PROTEIN-SERINE-THREONINE KINASES; RADIATION TOLERANCE; THREONINE;

EID: 0037444375     PISSN: 00085472     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (14)

1. Pierce, A. J., Stark, J. M., Araujo, F. D., Moynahan, M. E., Berwick, M., and Jasin, M. Double-strand breaks and tumorigenesis. Trends Cell Biol., 11: S52-S59, 2001.
2. Smith, G. C., and Jackson, S. P. The DNA-dependent protein kinase. Genes Dev., 13: 916-934, 1999.
3. Kurimasa, A., Kumano, S., Boubnov, N. V., Story, M. D., Tung, C. S., Peterson, S. R., and Chen, D. J. Requirement for the kinase activity of human DNA-dependent protein kinase catalytic subunit in DNA strand break rejoining. Mol. Cell. Biol., 19: 3877-3884, 1999.
4. Chan, D. W., and Lees-Miller, S. P. The DNA-dependent protein kinase is inactivated by autophosphorylation of the catalytic subunit. J. Biol. Chem., 271: 8936-8941, 1996.
5. Achari, Y., and Lees-Miller, S. P. Detection of DNA-dependent protein kinase in extracts from human and rodent cells. Methods Mol. Biol., 99: 85-97, 2000.
6. Giffin, W., Kwast-Welfeld, J., Rodda, D. J., Préfontaine, G. G., Traykova-Andonova, M., Zhang, Y., Weigel, N. L., Lefebvre, Y. A., and Haché, R. J. Sequence-specific DNA binding and transcription factor phosphorylation by Ku Autoantigen/DNA-dependent protein kinase. Phosphorylation of Ser-527 of the rat glucocorticoid receptor. J. Biol. Chem., 272: 5647-5658, 1997.
7. Chan, D. W., Ye, R., Veillette, C. J., and Lees-Miller, S. P. DNA-dependent protein kinase phosphorylation sites in Ku 70/80 heterodimer. Biochemistry, 38: 1819-1828, 1999.
8. Soubeyrand, S., Torrance, H., Giffin, W., Gong, W., Schild-Poulter, C., and Hache, R. J. Activation and autoregulation of DNA-PK from structured single-stranded DNA and coding end hairpins. Proc. Natl. Acad. Sci. USA, 98: 9605-9610, 2001.
9. Schild-Poulter, C., Pope, L., Giffin, W., Kochan, J. C., Ngsee, J. K., Traykova-Andonova, M., and Hache, R. J. G. The binding of Ku antigen to homeodomain proteins promotes their phosphorylation by DNA-dependent protein kinase. J. Biol. Chem., 276: 16848-16856, 2001.
10. Douglas, P., Sapkota, G. P., Morrice, N., Yu, Y., Goodarzi, A. A., Merkle, D., Meek, K., Alessi, D. R., and Lees-Miller, S. P. Identification of in vitro and in vivo phosphorylation sites in the catalytic subunit of the DNA-dependent protein kinase. Biochem. J., 368: 243-251, 2002.
11. Kirchgessner, C. U., Patil, C. K., Evans, J. W., Cuomo, C. A., Fried, L. M., Carter, T., Oettinger, M. A., and Brown, J. M. DNA-dependent kinase (p350) as a candidate gene for the murine SCID defect. Science (Wash. DC), 267: 1178-1183, 1995.
12. Kienker, L. J., Shin, E. K., and Meek, K. Both V(D)J recombination and radioresistance require DNA-PK kinase activity, though minimal levels suffice for V(D)J recombination. Nucleic Acids Res., 28: 2752-2761, 2000.
13. Kultz, D., and Chakravarty, D. Hyperosmolality in the form of elevated NaCl but not urea causes DNA damage in murine kidney cells. Proc. Natl. Acad. Sci. USA, 98: 1999-2004, 2001.
14. Chan, D. W., Chen, B. P., Prithivirajsingh, S., Kurimasa, A., Story, M. D., Qin, J., and Chen, D. J. Autophosphorylation of the DNA-dependent protein kinase catalytic subunit is required for rejoining of DNA double-strand breaks. Genes Dev., 16: 2333-2338, 2002.