Simultaneous expression of guinea pig UDP-glucuronosyltransferase 2B21 and 2B22 in COS-7 cells enhances UDP-glucuronosyltransferase 2B21-catalyzed morphine-6-glucuronide formation

Molecular Pharmacology

Volumn 60, Issue 5, 2001, Pages 1040-1048

  Ishii, Yuji ^a,c   Miyoshi, Aya ^a   Watanabe, Ryoichi ^a   Tsuruda, Kazuoki ^a   Tsuda, Minoru ^a   Yamaguchi Nagamatsu, Yuki ^a   Yoshisue, Kunihiro ^a   Tanaka, Mitsuko ^a   Maji, Daisuke ^a   Ohgiya, Satoru ^b   Oguri, Kazuta ^a  

^a KYUSHU UNIVERSITY   (Japan)

^b NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^c UNIVERSITY OF TSUKUBA   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; DETERGENT; GLUCURONOSYLTRANSFERASE; GLUCURONOSYLTRANSFERASE 2B21; GLUCURONOSYLTRANSFERASE 2B22; MORPHINE; MORPHINE 6 GLUCURONIDE; OLIGOMER; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DETOXIFICATION; DNA TRANSFECTION; DRUG GLUCURONIDATION; DRUG METABOLISM; DRUG PROTEIN BINDING; ENZYME KINETICS; ENZYME SPECIFICITY; ENZYME SUBSTRATE; ENZYME SUBUNIT; GUINEA PIG; METABOLIC REGULATION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OLIGOMERIZATION; PRIORITY JOURNAL; PROTEIN EXPRESSION;

EID: 0034752769     PISSN: 0026895X     EISSN: None     Source Type: Journal    
DOI: 10.1124/mol.60.5.1040     Document Type: Article

References (39)

1. Interaction among P450 enzymes when combined in reconstituted system: Formation of a 2B4-1A2 A2 complex with high affinity for NADPH-cytochrome P450 reductase
2. Expression and characterization of a novel UDP-glucuronosyltransferase, UGT2B9, from cynomolgus monkey
3. Assay of proteins in the presence of interfering materials
4. Purification of rat-liver microsomal UDP-glucuronyltransferase. Separation of two enzyme forms inducible by 3-methylcholanthrene or phenobarbital
5. Human UGT2B7 catalyzes morphine glucuronidation
6. Cloning and expression of a rat liver phenobarbital-inducible UDP-glucuronosyltransferase (2B12) with specificity for monoterpenoid alcohols
7. Glucuronidation of opioids, carboxylic acid-containing drugs, and hydroxylated xenobiotics catalyzed by expressed monkey UDP-glucuronosyltransferase 2B9 protein
8. Radiation inactivation analysis of microsomal UDP-glucuronosyltransferases catalysing mono- and diglucuronide formation of 3,6-dihydroxybenzo(a)pyrene and 3,6-dihydroxychrysene
9. Modulation of the number of ligand binding sites of UDP-glucuronyltransferase by the gel to liquid-crystal phase transition of phosphatidylcholines
10. Protein-protein interactions between UDP-glucuronosyltransferase isozymes in rat hepatic microsomes
11. Purification and characterization of a morphine UDP-Glucuronyltransferase isoform from untreated rat liver
12. Sequencing proteins from acrylamide gels
13. Crigler-Najjar syndrome type II is inherited both as a dominant and as a recessive trait
14. Species difference of site-selective glucuronidation of morphine
15. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
16. Protein measurement with the folin phenol reagent
17. Purification and immunochemical characterization of a low-pI form of UDP glucuronosyltransferase from mouse liver
18. The UDP glycosyltransferase gene superfamily: Recommended nomenclature update based on evolutionary divergence
19. Sequence from picomole quantities of proteins eiectroblotted onto polyvinylidene difluoride membranes
20. Genetic deficiency of androsterone UDP-glucuronosyltransferase activity in Wistar rats is due to the loss of enzyme protein
21. UDP-glucuronosyltransferase, the role of the amino terminus in dimerization
22. Multiplicity of deoxyribonucleic acid sequences with homology to a cloned complementary deoxyribonucleic acid coding for rat phenobarbital-inducible cytochrome P-450
23. Characteristic glucuronidation pattern of physiologic concentration of morphine in rat brain
24. High resolution two-dimensional electrophoresis of basic as well as acidic proteins
25. Purification of a phenobarbital-inducible UDP-glucuronosyltransferase isoform from dog liver which catalyzes morphine and testosterone glucuronidation
26. Molecular cloning of guinea pig CYP1A1: Complete primary structure and fast mobility of expressed protein on electrophoresis
27. Isolation of morphine from toad skin
28. Nonurea sodium dodecyl sulfate-polyacrylamide gel electrophoresis with high-molarity buffers for the separation of proteins and peptides
29. The molecular weights of UDP-glucuronyltransferase determined with radiation-inactivation analysis. A molecular model of bilirubin UDP-glucuronyltransferase
30. Isolation and purification of rat liver morphine UDP-glucuronosyltransferase
31. Cloning and expression of human liver UDP-glucuronosyltransferase in COS-1 cells. 3,4-catechol estrogens and estriol as primary substrates
32. Receptors for dopamine and somatostatin: Formation of hetero-oligomers with enhanced functional activity
33. Oral morphine in cancer patients: In vivo kinetics and in vitro hepatic glucuronidation
34. Investigation of the substrate specificity of a cloned expressed human bilirubin UDP-glucuronosyltransferase: UDP-sugar specificity and involvement in steroid and xenobiotic glucuronidation
35. Analgesic effect of morphine glucuronides
36. Interaction between cytochrome P450 and other drug metabolizing enzymes evidence for an association of CYP1A1 with microsomal epoxide hydrolase and UDP-glucuronosyltransferase
37. Cloning and characterization of rabbit liver UDP-glucuronosyltransferase cDNAs. Developmental and inducible expression of 4-hydroxybiphenyl UGT2B13
38. The synthesis of codeine and morphine glucuronides
39. Glucuronidation of codeine and morphine in human liver and kidney microsomes: Effect of inhibitors