Caspase inhibition shifts neuroepithelioma cell response to okadaic acid from apoptosis to an apoptotic-like form of death

Biochemical and Biophysical Research Communications

Volumn 303, Issue 2, 2003, Pages 469-474

  Romano, Elena ^a   Cannata, Stefano ^a   Di Bartolomeo, Sabrina ^a,b   Spinedi, Angelo ^a  

^a UNIVERSITY OF ROME TOR VERGATA   (Italy)

^b SAPIENZA UNIVERSITY OF ROME   (Italy)

Author keywords

Apoptosis; Caspase; Mitotic arrest; Neuroepithelioma; Okadaic acid; Premature chromosome condensation; Protein phosphatase

Indexed keywords

BENZYLOXYCARBONYLVALYLALANYLASPARTYL FLUOROMETHYL KETONE; CASPASE; OKADAIC ACID; PROTEIN P21; PROTEIN P53; TUMOR SUPPRESSOR PROTEIN;

APOPTOSIS; ARTICLE; CELL DEATH; CELL POPULATION; CELL STRUCTURE; CONTROLLED STUDY; DOWN REGULATION; DRUG EFFECT; ENZYME ACTIVATION; ENZYME INHIBITION; FLOW CYTOMETRY; HUMAN; HUMAN CELL; MITOSIS INHIBITION; NEUROEPITHELIOMA; PHENOTYPE; PREMATURE CHROMOSOME CONDENSATION; PRIORITY JOURNAL; PROTEIN FUNCTION; TUMOR CELL CULTURE; TUMOR SUPPRESSOR GENE; UPREGULATION;

CHANDIPURA VIRUS;

EID: 0037418732     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-291X(03)00358-9     Document Type: Article

References (31)

1. Bialojan C., Takai A. Inhibitory effect of a marine-sponge toxin, okadaic acid, on protein phosphatases. Specificity and kinetics. Biochem. J. 256:1988;283-290.
2. Wera S., Hemmings B.A. Serine/threonine protein phosphatases. Biochem. J. 311:1995;17-29.
3. Yamashita K., Yasuda H., Pines J., Yasumoto K., Nishitani H., Ohtsubo M., Hunter T., Sugimura T., Nishimoto T. Okadaic acid, a potent inhibitor of type 1 and type 2A protein phosphatases, activates cdc2/H1 kinase and transiently induces a premature mitosis-like state in BHK21 cells. EMBO J. 9:1990;4331-4338.
4. Zheng B., Woo C.F., Kuo J.F. Mitotic arrest and enhanced nuclear protein phosphorylation in human leukemia K562 cells by okadaic acid, a potent protein phosphatase inhibitor and tumor promoter. J. Biol. Chem. 266:1991;10031-10034.
5. 2 -phase to induce premature mitosis in HeLa cells Exp. Cell Res. 227:1996;165-169.
6. Boe R., Gjertsen B.T., Vintermyr O.K., Houge G., Lanotte M., Døskeland S.O. The protein phosphatase inhibitor okadaic acid induces morphological changes typical of apoptosis in mammalian cells. Exp. Cell Res. 195:1991;237-246.
7. Cip1/Waf1 and retinoblastoma protein during okadaic acid-induced apoptosis is coupled with induction of Bax protein in human breast carcinoma cells Cell Growth Differ. 7:1996;1599-1607.
8. Morimoto Y., Ohba T., Kobayashi S., Haneji T. The protein phosphatase inhibitors okadaic acid and calyculin A induce apoptosis in human osteoblastic cells. Exp. Cell Res. 230:1997;181-186.
9. Li D.W., Fass U., Huizar I., Spector A. Okadaic acid-induced lens epithelial cell apoptosis requires inhibition of phosphatase-1 and is asociated with induction of gene expression including p53 and bax. Eur. J. Biochem. 257:1998;351-361.
10. Spinedi A., Di Bartolomeo S., Di Sano F., Rodolfo C., Ambrosino A., Piacentini M. Ceramide accumulation precedes caspase-dependent apoptosis in CHP-100 neuroepithelioma cells exposed to the protein phosphatase inhibitor okadaic acid. Cell Death Differ. 6:1999;618-623.
11. Li D.W., Xiang H., Mao Y.W., Wang J., Fass U., Zhang X.Y., Xu C. Caspase-3 is actively involved in okadaic acid-induced lens epithelial cell apoptosis. Exp. Cell Res. 266:2001;279-291.
12. Sandal T., Ahlgren R., Lillehaug J., Døskeland S.O. Establishment of okadaic acid resistant cell clones using a cDNA expression library. Cell Death Differ. 8:2001;754-766.
13. Inomata M., Saijo N., Kawashima K., Kaneko A., Fujiwara Y., Kunikane H., Tanaka Y. Induction of apoptosis in cultured retinoblastoma cells by the protein phosphatase inhibitor, okadaic acid. J. Cancer Res. Clin. Oncol. 121:1995;729-738.
14. Nuydens R., de Jong M., Van Den Kieboom G., Heers C., Dispersyn G., Cornelissen F., Nuyens R., Borgers M., Geerts H. Okadaic acid-induced apoptosis in neuronal cells: evidence for an abortive mitotic attempt. J. Neurochem. 70:1998;1124-1133.
15. Lerga A., Richard C., Delgado M.D., Canelles M., Frade P., Cuadrado M.A., Leon J. Apoptosis and mitotic arrest are two independent effects of the protein phosphatases inhibitor okadaic acid in K562 leukemia cells. Biochem. Biophys. Res. Commun. 260:1999;256-264.
16. Yan Y., Shay J.W., Wright W.E., Mumby M.C. Inhibition of protein phosphatase activity induces p53-dependent apoptosis in the absence of p53 transactivation. J. Biol. Chem. 272:1997;15220-15226.
17. Zeng Y.X., El-Deiry W.S. Regulation of p21WAF1/CIP1 expression by p53-independent pathways. Oncogene. 12:1996;1557-1564.
18. Milczarek G.J., Chen W., Gupta A., Martinez J.D., Bowden G.T. Okadaic acid mediates p53 hyperphosphorylation and growth arrest in cells with wild-type p53 but increases aberrant mitoses in cells with non-functional p53. Carcinogenesis. 20:1999;1043-1048.
19. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:1951;265-275.
20. Darzynkiewicz Z., Bedner E., Traganos F. Difficulties and pitfalls in analysis of apoptosis. Methods Cell Biol. 63:2001;527-546.
21. Kasten M.M., Giordano A. pRb and the cdks in apoptosis and the cell cycle. Cell Death Differ. 5:1998;132-140.
22. Harbour J.W., Dean D.C. Rb function in cell-cycle regulation and apoptosis. Nat. Cell Biol. 2:2000;E65-E67.
23. Millward T.A., Zolnierowicz S., Hemmings B.A. Regulation of protein kinase cascades by protein phosphatase 2A. Trends Biochem. Sci. 24:1999;186-191.
24. Nelson D.A., Krucher N.A., Ludlow J.W. High molecular weight protein phosphatase type 1 dephosphorylates the retinoblastoma protein. J. Biol. Chem. 272:1997;4528-4535.
25. Rubin E., Mittnacht S., Villa-Moruzzi E., Ludlow J.W. Site-specific and temporally-regulated retinoblastoma protein dephosphorylation by protein phosphatase type 1. Oncogene. 20:2001;3776-3785.
26. Yan Y., Mumby M.C. Distinct roles for PP1 and PP2A in phosphorylation of the retinoblastoma protein. PP2A regulates the activities of G(1) cyclin-dependent kinases. J. Biol. Chem. 274:1999;3124-31917.
27. Ekert P.G., Silke J., Vaux D.L. Caspase inhibitors. Cell Death Differ. 6:1999;1081-1086.
28. Leist M., Jäättelä M. Four deaths and a funeral: from caspases to alternative mechanisms. Nat. Rev. Mol. Cell Biol. 2:2002;589-598.
29. Di Bartolomeo S., Di Sano F., Piacentini M., Spinedi A. Apoptosis induced by doxorubicin in neurotumor cells is divorced from drug effects on ceramide accumulation and may involve cell cycle-specific caspase activation. J. Neurochem. 75:2000;532-539.
30. Boutillier A.L., Trinh E., Loeffler J.P. Caspase-dependent cleavage of the retinoblastoma protein is an early step in neuronal apoptosis. Oncogene. 19:2000;2171-2178.
31. Chau B.N., Borges H.L., Chen T.T., Masselli A., Hunton I.C., Wang J.Y. Signal-dependent protection from apoptosis in mice expressing caspase-resistant Rb. Nat. Cell Biol. 4:2002;757-765.