Structure of Cdc42 in a complex with the GTPase-binding domain of the cell polarity protein, Par6

EMBO Journal

Volumn 22, Issue 5, 2003, Pages 1125-1133

  Garrard, Sarah M ^a   Capaldo, Christopher T ^a   Gao, Lin ^a   Rosen, Michael K ^b   Macara, Ian G ^a   Tomchick, Diana R ^b  

^a UNIVERSITY OF VIRGINIA   (United States)

^b UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

Crystallography; Fluorescence; NMR; Polarity; Rho GTPases

Indexed keywords

PROTEIN; PROTEIN CDC42; PROTEIN PAR6; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BINDING SITE; CELL POLARITY; CELL SEPARATION; COMPLEX FORMATION; CONTROLLED STUDY; CRYSTAL STRUCTURE; CRYSTALLIZATION; DOG; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN MOTIF; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; STRUCTURE ANALYSIS;

AMINO ACID SEQUENCE; ANIMALS; BINDING SITES; CDC42 GTP-BINDING PROTEIN; CELL LINE; CELL POLARITY; CRYSTALLOGRAPHY, X-RAY; EPITHELIAL CELLS; FLUORESCENCE RESONANCE ENERGY TRANSFER; MACROMOLECULAR SUBSTANCES; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN STRUCTURE, TERTIARY; PROTEINS; RECOMBINANT FUSION PROTEINS; SEQUENCE ALIGNMENT;

ANIMALIA; CANIS FAMILIARIS; EUKARYOTA; MAMMALIA; METAZOA; SACCHAROMYCETALES;

EID: 0037416217     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/cdg110     Document Type: Article

References (40)

1. Abdul-Manan, N., Aghazadeh, B., Liu, G.A., Majumdar, A., Ouerfelli, O., Siminovitch, K.A. and Rosen, M.K. (1999) Structure of Cdc42 in complex with the GTPase-binding domain of the 'Wiskott-Aldrich syndrome' protein. Nature, 399, 379-383.
2. Brünger, A.T. et al. (1998) Crystallography and NMR system: a new software suite for macromolecular structure determination. Acta Ctystallogr. D, 54, 905-921.
3. Buchwald, G., Hostinova, E., Rudolph, M.G., Kraemer, A., Sickmann, A., Meyer, H.E., Scheffzek, K. and Wittinghofer, A. (2001) Conformational switch and role of phosphorylation in PAK activation. Mol. Cell. Biol., 21, 5179-5189.
4. Burbelo, P.D., Drechsel, D. and Hall, A. (1995) A conserved binding motif defines numerous candidate target proteins for both Cdc42 and Rac GTPases. J. Biol. Chem., 270, 29071-29074.
5. CCP4 (1994) The CCP4 suite of programs for protein crystallography. Acta Crystallogr. D, 50, 760-763.
6. Cowtan, K. and Main, P. (1998) Miscellaneous algorithms for density modification. Acta Crystallogr. D, 54, 487-493.
7. Dyson, H.J. and Wright, P.E. (2002) Coupling of folding and binding for unstructured proteins. Curr. Opin. Struct. Biol., 12, 54-60.
8. Etienne-Manneville, S. and Hall, A. (2001) Integrin-mediated activation of Cdc42 controls cell polarity in migrating astrocytes through PKC. Cell, 106, 489-498.
9. French, S. and Wilson, K. (1978) On the treatment of negative intensity observations. Acta Crystallogr. A, 34, 517-525.
10. Gao, L., Joberty, G. and Macara, I.G. (2002) Assembly of epithelial tight junctions is negatively regulated by Par6. Curr. Biol., 12, 221-225.
11. Gosser, Y.Q., Nomanbhoy, T.K., Aghazadeh, B., Manor, D., Combs, C., Cerione, R.A. and Rosen, M.K. (1997) C-terminal binding domain of Rho GDP-dissociation inhibitor directs N-terminal inhibitory peptide to GTPases. Nature, 387, 814-819.
12. Gotta, M., Abraham, M.C. and Ahringer, J. (2001) CDC-42 controls early cell polarity and spindle orientation in C. elegans. Curr. Biol., 11, 482-488.
13. Heim, R. and Tsien, R.Y. (1996) Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer. Curr. Biol., 6, 178-182.
14. Hung, T.J. and Kemphues, K.J. (1999) PAR-6 is a conserved PDZ domain-containing protein that colocalizes with PAR-3 in Caenorhabditis elegans embryos. Development, 126, 127-135.
15. Huynh, J.R., Petronczki, M., Knoblich, J.A. and St Johnston, D. (2001) Bazooka and PAR-6 are required with PAR-1 for the maintenance of oocyte fate in Drosophila. Curr. Biol., 11, 901-906.
16. Joberty, G., Petersen, C., Gao, L. and Macara, I.G. (2000) The cell-polarity protein Par6 links Par3 and atypical protein kinase C to Cdc42. Nat. Cell Biol., 2, 531-539.
17. Jones, T.A., Zou, J.Y., Cowan, S.W. and Kjeldgaard, M. (1991) Improved methods for building protein models in electron density maps and the location of errors in these models. Acta Crystallogr. A, 47, 110-119.
18. Kay, A.J. and Hunter, C.P. (2001) CDC-42 regulates PAR protein localization and function to control cellular and embryonic polarity in C. elegans. Curr. Biol., 11, 474-481.
19. Kay, L.E., Keifer, P. and Saarinen, T. (1992) Pure absorption gradient enhanced heteronuclear single quantum correlation spectroscopy with improved sensitivity. J. Am. Chem. Soc., 114, 10663-10665.
20. Kim, A.S., Kakalis, L.T., Abdul-Manan, N., Liu, G.A. and Rosen, M.K. (2000) Autoinhibition and activation mechanisms of the Wiskott-Aldrich syndrome protein. Nature, 404, 151-158.
21. Lei, M., Lu, W., Meng, W., Parrini, M.C., Eck, M.J., Mayer, B.J. and Harrison, S.C. (2000) Structure of PAK1 in an autoinhibited conformation reveals a multistage activation switch. Cell, 102, 387-397.
22. Lin, D., Edwards, A.S., Fawcett, J.P., Mbamalu, G., Scott, J.D. and Pawson, T. (2000) A mammalian PAR-3-PAR-6 complex implicated in Cdc42/Rac1 and aPKC signalling and cell polarity. Nat. Cell Biol., 2, 540-547.
23. Morreale, A., Venkatesan, M., Mott, H.R., Owen, D., Nietlispach, D., Lowe, P.N. and Laue, E.D. (2000) Structure of Cdc42 bound to the GTPase binding domain of PAK. Nat. Struct. Biol., 7, 384-388.
24. Mott, H.R., Owen, D., Nietlispach, D., Lowe, P.N., Manser, E., Lim, L. and Laue, E.D. (1999) Structure of the small G protein Cdc42 bound to the GTPase-binding domain of ACK. Nature, 399, 384-388.
25. Nassar, N., Hoffman, G.R., Manor, D., Clardy, J.C. and Cerione, R.A. (1998) Structures of Cdc42 bound to the active and catalytically compromised forms of Cdc42GAP. Nat. Struct. Biol., 5, 1047-1052.
26. Navaza, J. (1994) AMORE: an automated package for molecular replacement. Acta Crystallogr. A, 50, 157-163.
27. Nobes, C.D. and Hall, A. (1999) Rho GTPases control polarity, protrusion and adhesion during cell movement. J. Cell Biol., 144, 1235-1244.
28. Ohno,S. (2001) Intercellular junctions and cellular polarity: the PAR-aPKC complex, a conserved core cassette playing fundamental roles in cell polarity. Curr. Opin. Cell Biol., 13, 641-648.
29. Otwinoski, Z. and Minor, W. (1997) Processing of X-ray diffraction data collected in oscillation mode. Methods Enzymol., 276, 307-326.
30. Owen, D., Mott, H.R., Laue, E.D. and Lowe, P.N. (2000) Residues in Cdc42 that specify binding to individual CRIB effector proteins. Biochemistry, 39, 1243-1250.
31. Petronczki, M. and Knoblich, J.A. (2001) DmPAR-6 directs epithelial polarity and asymmetric cell division of neuroblasts in Drosophila. Nat. Cell Biol., 3, 43-49.
32. Qiu, R.G., Abo, A. and Steven Martin, G. (2000) A human homolog of the C. elegans polarity determinant Par-6 links Rac and Cdc42 to PKCζ signaling and cell transformation. Curr. Biol., 10, 697-707.
33. Ranganathan, R. and Ross, E.M. (1997) PDZ domain proteins - scaffolds for signaling complexes. Curr. Biol., 7, R770-R773.
34. Read, R.J. and Kleywegt, G.J. (2001) Density modification: theory and practice. In Turk, D. and Johnson, L. (eds), Methods Macromolecular Crystallography. IOS Press, Amsterdam, The Netherlands, pp. 123-135.
35. Selvin, P.R. (1995) Fluorescence resonance energy transfer. Methods Enzymol., 246, 300-334.
36. Suzuki, A. et al. (2001) Atypical protein kinase C is involved in the evolutionarily conserved par protein complex and plays a critical role in establishing epithelia-specific junctional structures. J. Cell Biol., 152, 1183-1196.
37. Watts, J.L., Etemad-Moghadam, B., Guo, S., Boyd, L., Draper, B.W., Mello,C.C., Priess, J.R. and Kemphues, K.J. (1996) Par-6, a gene involved in the establishment of asymmetry in early C. elegans embryos, mediates the asymmetric localization of PAR-3. Development, 122, 3133-3140.
38. Wright, P.E. and Dyson, H.J. (1999) Intrinsically unstructured proteins: re-assessing the protein structure-function paradigm. J. Mol. Biol., 293, 321-331.
39. Wuthrich, K. (1986) NMR of Proteins and Nucleic Acids. John Wiley & Sons, New York, NY, pp. 162-175.
40. Yamanaka, T. et al. (2001) PAR-6 regulates aPKC activity in a novel way and mediates cell-cell contact-induced formation of the epithelial junctional complex. Genes Cells, 6, 721-731.