Endogenous assays of DNA methyltransferases: Evidence for differential activities of DNMT1, DNMT2, and DNMT3 in mammalian cells in vivo

Molecular and Cellular Biology

Volumn 23, Issue 8, 2003, Pages 2709-2719

  Liu, Kui ^a   Wang, Yun Fei ^a   Cantemir, Carmen ^a   Muller, Mark T ^a  

^a OHIO STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

5 AZA 2' DEOXYCYTIDINE; ANTIBODY; DNA METHYLTRANSFERASE; DNA METHYLTRANSFERASE 1; DNA METHYLTRANSFERASE 2; DNA METHYLTRANSFERASE 3; DNA METHYLTRANSFERASE 3A; DNA METHYLTRANSFERASE 3B; GENOMIC DNA; UNCLASSIFIED DRUG;

ALTERNATIVE RNA SPLICING; ANIMAL CELL; ARTICLE; BINDING AFFINITY; CATALYSIS; CELL LINE; CHROMATIN; CONTROLLED STUDY; CPG ISLAND; DNA METHYLATION; DNA SEQUENCE; ENZYME ACTIVITY; ENZYME ASSAY; GENE TARGETING; GENETIC CONSERVATION; HUMAN; HUMAN CELL; IN VIVO STUDY; MAMMAL CELL; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DNA BINDING; SEQUENCE HOMOLOGY;

ANIMALS; CELL LINE; DNA (CYTOSINE-5-)-METHYLTRANSFERASE; DNA METHYLATION; DROSOPHILA PROTEINS; HELA CELLS; HUMANS; IMMUNOBLOTTING; KINETICS; MICE;

ANIMALIA; MAMMALIA; STAPHYLOCOCCUS PHAGE 3A;

EID: 0037383398     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.23.8.2709-2719.2003     Document Type: Article

References (40)

1. Aoki, A., I. Suetake, J. Miyagawa, T. Fujio, T. Chijiwa, H. Sasaki, and S. Tajima. 2001. Enzymatic properties of de novo type mouse DNA (cytosine-5) methyltransferases. Nucleic Acids Res. 29:3506-3512.
2. Baylin, S. B., M. Esteller, M. R. Rountree, K. E. Bachman, K. Schuebel and J. G. Herman. 2001. Aberrant patterns of DNA methylation, chromatin formation and gene expression in cancer. Hum. Mol. Genet. 10:687-692.
3. Belinsky, S. A., K. J. Nikula, W. A. Palmisano, R. Michels, G. Saccomanno, E. Gabrielson, S. B. Baylin, and J. G. Herman. 1998. Aberrant methylation of p16INK4a is an early event in lung cancer and a potential biomarker for early diagnosis. Proc. Natl. Acad. Sci. USA 95:11891-11896.
4. Bestor, T. 2000. The DNA methyltransferases of mammals. Hum. Mol. Genet. 9:2395-2402.
5. Bonfils, C., N. Beaulieu, E. Chan, J. Cotton-Montpetit, and A. R. MacLeod. 2000. Characterization of the human DNA methyltransferase splice variant Dnmtlb. J. Biol. Chem. 275:10754-10760.
6. Brank, A. S., R. Eritja, R. G. Barcia, V. E. Marquez, and J. K. Christman. 2002. Inhibition of HhaI DNA (Cytosine-C5) methyltransferase by oligodeoxyribonucleotides containing 5-aza-2′-deoxycytidine: examination of the intertwined roles of co-factor, target, transition state structure and enzyme conformation. J. Mol. Biol. 323:53-67.
7. Costello, J. F., and C. Plass. 2001. Methylation matters. J. Med. Genet. 38:285-303.
8. Dong, A. Y., J. A. Yoder, A. Zhang, L. Zhou, T. H. Bestor, and X. Cheng. 2001. Structure of human DNMT2, an enigmatic DNA methyltransferase homolog that displays denaturant-resistant binding to DNA. Nucleic Acids Res. 29:439-448.
9. Ferguson, A., P. Vertino, J. Spitzner, S. Baylin, M. Muller, and N. Davidson. 1997. Role of estrogen receptor gene demethylation and DNA methyltransferase-DNA adduct formation in 5′-aza-2′ deoxycytidine-induced cytoxicity in human breast cancer cells. J. Biol. Chem. 272:32260-32266.
10. Gabbara, S., and A. S. Bhagwat. 1995. The mechanism of inhibition of DNA (cytosine-5-)-methyltransferase by 5-azacytosine is likely to involve methyl transfer to the inhibitor. Biochem. J. 307:87-92.
11. Gowher, H., O. Leismann, and A. Jeltsch. 2000. DNA of Drosophila melanogaster contains 5-methylcytosine. EMBO J. 19:6918-6923.
12. Hsieh, C.-L. 1999. In vivo activity of murine de novo methyltransferases, Dnmt3a and Dnmt3b. Mol. Cell. Biol. 19:8211-8218.
13. Hsu, D.-W., M.-J. Lin, T.-L. Lee, S.-C. Wen, X. Chen, and C.-K. J. Shen. 1999. Two major forms of DNA (cytosine-5) methyltransferase in human somatic tissues. Proc. Natl. Acad. Sci. USA 96:9751-9756.
14. Interthal, H., J. J. Pouliot, and J. J. Champoux. 2001. The tyrosyl-DNA phosphodiesterase Tdp 1 is a member of the phospholipase D superfamily. Proc. Natl. Acad. Sci. USA 98:12009-12014.
15. Juttermann, R., E. Li, and R. Jaenisch. 1994. Toxicity of 5-aza-2′-deoxycytidine to mammalian cells is mediated primarily by covalent trapping of DNA methyltransferase rather than DNA demethylation. Proc. Natl. Acad. Sci. USA 91:11797-11801.
16. Karpf, A. R., B. C. Moore, T. O. Ririe, and D. A. Jones. 2001. Activation of the p53 DNA damage response pathway after inhibition of DNA methyltransferase by 5-aza-2′-deoxycytidine. Mol. Pharmacol. 59:751-757.
17. Katz, A. J. 1985. Genotoxicity of 5-azacytidine in somatic cells of Drosophila. Mutat. Res. 143:195-199.
18. Li, E., T. Bestor, and R. Jaenisch. 1992. Targeted mutation of the DNA methyltransferase gene results in embryonic lethality. Cell 69:915-926.
19. Lyko, F., B. H. Ramsahoye, and R. Jaenisch. 2000. DNA methylation in Drosophila melanogaster. Nature 408:538-540.
20. Mao, Y., S. Okada, L. Chang, and M. T. Muller. 2000. p53′ dependence of topoisomerase I recruitment in vivo. Cancer Res. 60:4538-4543.
21. Okano, M., D. Bell, D. Haber, and E. Li. 1999. DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development. Cell 99:247-257.
22. Okano, M., S. Xie, and E. Li. 1998. Dnmt2 is not required for de novo and maintenance methylation of viral DNA in ES cells. Nucleic Acids Res. 26:2536-2540.
23. Ordway, J. M., and T. Curran. 2002. Methylation matters: modeling a manageable genome. Cell Growth Differ. 13:149-162.
24. Osgood, C. J., and S. M. Seward. 1989. 5-Azacytidine induces sex chromosome loss and interchange in immature germ cells of Drosophila mei-9 males. Environ. Mol. Mutag. 14:135-145.
25. Pontecorvo, G., A. Avitabile, G. Esposito, G. Migliaccio, and M. Carfagna. 1992. Induced crossing-over in Drosophila melanogaster germ cells of DNA repair-proficient and repair-deficient (mei-9L1) males following larval feeding with 5-azacytidine and mitomycin C. Mutat. Res. 266:93-98.
26. Pouliot, J. J., K. C. Yao, C. A. Robertson, and H. A. Nash. 1999. Yeast gene for a tyr-DNA phosphodiesterase that repairs topoisomerase I complexes. Science 286:552-555.
27. Rhee, I., K. Jair, R. Yen, C. Lengauer, J. Herman, K. Kinzler, B. Vogelstein, S. Baylin, and K. E. Schuebel. 2001. CpG methylation is maintained in human cancer cells lacking DNMT1. Nature 404:1003-1007.
28. Rhee, I., K. E. Bachman, B. H. Park, K. W. Jair, R. W. Yen, K. E. Schuebel, H. Cui, A. P. Feinberg, C. Langeur, K. W. Kinzler, S. B. Baylin, and B. Vogelstein. 2002. DNMT1 and DNMT3b cooperate to silence genes in human cancer cells. Nature 416:552-556.
29. Robertson, K. D. 2002. DNA methylation and chromatin - unraveling the tangled web. Oncogene 21:5361-5379.
30. Robertson, K. D. 2001. DNA methylation, methyltransferases, and cancer. Oncogene 20:3139-3155.
31. Robertson, K., et al. 1999. The human DNA methyltransferases (DNMTs) 1, 3a and 3b: coordinate mRNA expression in normal tissues and overexpression in tumors. Nucleic Acids Res. 27:2291-2298.
32. Rosenstein, B. S., D. Subramanian, and M. T. Muller. 1997. The involvement of topoisomerase I in the induction of DNA-protein crosslinks and DNA single-strand breaks in cells of ultraviolet-irradiated human and frog cell lines. Radiat. Res. 148:575-579.
33. Santi, D., C. E. Garrett, and P. J. Barr. 1983. On the mechanism of inhibition of DNA-cytosine methyltransferases by cytosine analogs. Cell 33:9-15.
34. Santi, D. V., A. Normant, and C. E. Garrett. 1984. Covalent bond formation between a DNA-cytosine methyltransferase and DNA containing 5-azacytosine. Proc. Natl. Acad. Sci. USA 81:6993-6997.
35. Subramanian, D., E. Kraut, A. Staubus, D. Young, and M. T. Muller. 1995. Analysis of topoisomerase I complexes in patients being treated with topotecan. Cancer Res. 55:2097-2103.
36. Subramanian, D., C. Sommer Furbee, and M. T. Muller. 2000. ICE bioassay. Isolating in vivo complexes of enzyme to DNA. Methods Mol. Biol. 95:137-147.
37. Subramanian, D., B. Rosenstein, and M. T. Muller. 1998. Ultraviolet-induced DNA damage stimulates topoisomerase I-DNA complex formation in vivo: possible relationship with DNA repair. Cancer Res. 58:976-984.
38. Trask. D. K., J. A. DiDonato, and M. T. Muller. 1984. Rapid detection and isolation of covalent DNA/protein complexes: application to topoisomerase I and II. EMBO J. 3:671-676.
39. Trask. D. K., and M. T. Muller. 1988. Stabilization of type I topoisomerase-DNA covalent complexes by actinomycin D. Proc. Natl. Acad. Sci. USA 85:1417-1421.
40. Yang, S., A. B. Burgin, B. N. Huizenga, C. A. Robertson, K. C. Yao, and H. A. Nash. 1996. A eukaryotic enzyme that can disjoin dead-end covalent complexes between DNA and type I topoisomerases. Proc. Natl. Acad. Sci. USA 93:11534-11539.