Mechanism of action of Gq to inhibit Gβγ modulation of CaV2.2 calcium channels: Probed by the use of receptor-Gα tandems

Molecular Pharmacology

Volumn 63, Issue 4, 2003, Pages 832-843

  Bertaso, Federica ^a   Ward, Richard J ^b   Viard, Patricia ^a   Milligan, Graeme ^b   Dolphin, Annette C ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b UNIVERSITY OF GLASGOW   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

2 [1 (3 DIMETHYLAMINOPROPYL) 3 INDOLYL] 3 (3 INDOLYL)MALEIMIDE; ALPHA 2 ADRENERGIC RECEPTOR; AMINO ACID; CALCIUM CHANNEL N TYPE; G PROTEIN COUPLED RECEPTOR; GUANINE NUCLEOTIDE BINDING PROTEIN; PERTUSSIS TOXIN; PHORBOL DIBUTYRATE; PHOSPHOLIPASE C; PROTEIN KINASE C; CALCIUM CHANNEL; GUANINE NUCLEOTIDE BINDING PROTEIN ALPHA SUBUNIT; HETEROTRIMERIC GUANINE NUCLEOTIDE BINDING PROTEIN; HYBRID PROTEIN; INHIBITORY GUANINE NUCLEOTIDE BINDING PROTEIN; RECEPTOR, ADRENERGIC, ALPHA 2A; RECEPTOR, ADRENERGIC, ALPHA-2A;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL STRAIN COS7; CONTROLLED STUDY; DRUG RECEPTOR BINDING; GENETIC TRANSFECTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; RECEPTOR INTRINSIC ACTIVITY; SODIUM CURRENT; ANIMAL; CELL STRAIN COS1; METABOLISM; PHOSPHORYLATION;

ANIMALS; CALCIUM CHANNELS; COS CELLS; GTP-BINDING PROTEIN ALPHA SUBUNITS, GI-GO; GTP-BINDING PROTEIN ALPHA SUBUNITS, GQ-G11; HETEROTRIMERIC GTP-BINDING PROTEINS; PHOSPHORYLATION; PROTEIN KINASE C; RECEPTORS, ADRENERGIC, ALPHA-2; RECOMBINANT FUSION PROTEINS; TRANSFECTION;

EID: 0037380799     PISSN: 0026895X     EISSN: None     Source Type: Journal    
DOI: 10.1124/mol.63.4.832     Document Type: Article

References (40)

1. 2A-adrenoceptor. Biochemistry 37:11555-11562.
2. Barrett CF and Rittenhouse AR (2000) Modulation of N-type calcium channel activity by G-proteins and protein kinase C. J Gen Physiol 115:277-286.
3. i proteins. J Biol Chem 273:10367-10375.
4. o: antisense oligonucleotide studies. J Physiol (Lond) 470:1-11.
5. Canti C, Bogdanov Y, and Dolphin AC (2000) Interaction between G proteins and accessory β subunits in the regulation of α1B calcium channels in Xenopus oocytes. J Physiol (Lond) 527:419-432.
6. Canti C, Davies A, Berrow NS, Butcher AJ, Page KM, and Dolphin AC (2001) Evidence for two concentration-dependent processes for β subunit effects on α1B calcium channels. Biophys J 81:1439-1451.
7. Canti C, Page KM, Stephens GJ, and Dolphin AC (1999) Identification of residues in the N terminus of α1B critical for inhibition of the voltage-dependent calcium channel by Gβγ. J Neurosci 19:6855-6864.
8. 11 J Pharmacol Exp Ther 302:1080-1088.
9. i2. J Biol Chem 275:23693-23699.
10. Chabre O, Conklin BR, Brandon S, Bourne HR, and Limbird LE (1994) Coupling of the α2A-adrenergic receptor to multiple G-proteins. A simple approach for estimating receptor-G-protein coupling efficiency in a transient expression system. J Biol Chem 269:5730-5734.
11. Conklin BR, Farfel Z, Lustig KD, Julius D, and Bourne HR (1993) Substitution of three amino acids switches receptor specificity of Gq alpha to that of Gi alpha. Nature (Lond) 363:274-276.
12. Conklin BR, Herzmark P, Ishida S, Voyno-Yasenetskaya TA, Sun Y, Farfel Z, and Bourne HR (1996) Carboxyl-terminal mutations of Gq α and Gs α that alter the fidelity of receptor activation. Mol Pharmacol 50:885-890.
13. Cooper CB, Arnot MI, Feng ZP, Jarvis SE, Hamid J, and Zamponi GW (2000) Cross-talk between G-protein and protein kinase C modulation of N-type calcium channels is dependent on the G-protein β subunit isoform. J Biol Chem 275:40777-40781.
14. 2+ channels in rat sympathetic neurones. J Physiol 518:47-53.
15. Diversé-Pierluissi M, Goldsmith PK, and Dunlap K (1995) Transmitter-mediated inhibition of N-type calcium channels in sensory neurons involves multiple GTP-binding proteins and subunits. Neuron 14:191-200.
16. Dolphin AC (1995) Voltage-dependent calcium channels and their modulation by neurotransmitters and G proteins: G. L. Brown prize lecture. Exp Physiol 80:1-36.
17. Dorn GW 2nd, Oswald KJ, McKluskey TS, Kuhel DG and Liggett SB (1997) Alpha 2A-adrenergic receptor stimulated calcium release is transduced by Gi-associated G(beta gamma)-mediated activation of phospholipase C. Biochemistry 36:6415-6423.
18. q. J Biol Chem 275:1327-1336.
19. i-proteins do so in situ. Eur J Neurosci 14:283-292.
20. Green A, Johnson JL, and Milligan G (1990) Down-regulation of Gi sub-types by prolonged incubation of adipocytes with an A1 adenosine receptor agonist. J Biol Chem 265:5206-5210.
21. q-deficient mice. J Neurosci 20:3973-3979.
22. Hamid J, Nelson D, Spaetgens R, Dubel S, Snutch TP, and Zamponi G (1999) Identification of an integration center for cross-talk between protein kinase C and G protein modulation of N type calcium channels. J Biol Chem 274:6195-6202.
23. 2+ channels by G-protein Bgamma subunits. Nature (Lond) 380:258-262.
24. Hille B (1994) Modulation of ion-channel function by G-protein-coupled receptors. Trends Neurol Sci 17:531-536.
25. 2+ channels in rat sympathetic neurons. J Neurosci 19:4755-4761.
26. 2+ channels. Neuron 22:819-829.
27. Kammermeier PJ, Ruiz-Velasco V, and Ikeda SR (2000) A voltage-independent calcium current inhibitory pathway activated by muscarinic agonists in rat sympathetic neurons requires both Galpha Q/11 and Gbeta gamma. J Neurosci 20:5623-5629.
28. Liang M, Eason MG, Jewell-Motz EA, Williams MA, Theiss CT, Dorn GW 2nd and Liggett SB (1998) Phosphorylation and functional desensitization of the α2a-adrenergic receptor by protein kinase C. Mol Pharmacol 54:44-49.
29. Meir A, Bell DC, Stephens GJ, Page KM, and Dolphin AC (2000) Calcium channel β subunit promotes voltage-dependent modulation of α1B by Gβγ. Biophys J 79:731-746.
30. o in neuroblastoma X glioma hybrid cells: independent regulation during cyclic AMP-induced differentiation. J Neurochem 55:1-9.
31. Puri TS, Gerhardstein BL, Zhao XL, Ladner MB, and Hosey MM (1997) Differential effects of subunit interactions on protein kinase A- and C-mediated phosphorylation of L-type calcium channels. Biochemistry 36:9605-9615.
32. Sautel M and Milligan G (1998) Loss of activation of Gs but Not Gi following expression of an Alpha2A-adrenoceptor-Gi1alpha fusion protein. FEBS Lett 436:46-50.
33. Seifert R, Wenzel-Seifert K, and Kobilka BK (1999) GPCR-Gα fusion proteins: molecular analysis of receptor-G-protein coupling. Trends Pharmacol Sci 20:383-389.
34. 2+ channel modulation. J Neurophysiol 76:311-20.
35. Simen AA, Lee CC, Simen BB, Bindokas VP, and Miller RJ (2001) The C terminus of the Ca channel alpha1b subunit mediates selective inhibition by G-protein-coupled receptors. J Neurosci 21:7587-7597.
36. Stephens GJ, Brice NL, Berrow NS, and Dolphin AC (1998) Facilitation of rabbit α1b calcium channels: involvement of endogenous Gβgamma subunits. J Physiol (Lond) 509:15-27.
37. z fusion protein. J Biol Chem 275:4166-4170.
38. i1alpha. Cell Signal 14:139-144.
39. 2. Nature (Lond) 419:947-952.
40. 1 subunit. Nature (Lond) 385:442-446.