The N-terminal 24 amino acids of the p55 gamma regulatory subunit of phosphoinositide 3-kinase binds Rb and induces cell cycle arrest

Molecular and Cellular Biology

Volumn 23, Issue 5, 2003, Pages 1717-1725

  Xia, Xianmin ^a,c   Cheng, Aiwu ^b,d   Akinmade, Damilola ^a,b   Hamburger, Anne W ^a,b  

^a UNIVERSITY OF MARYLAND   (United States)

^b UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

^c Protein Tech Group   (United States)

^d NIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PHOSPHATIDYLINOSITOL 3 KINASE; PROTEIN P55; PROTEIN SUBUNIT; RETINOBLASTOMA PROTEIN;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; CELL CULTURE; CELL CYCLE; CELL CYCLE G0 PHASE; CELL CYCLE G1 PHASE; CHEMICAL MODIFICATION; CONTROLLED STUDY; DNA SYNTHESIS; EMBRYO; ENZYME REGULATION; GENETIC TRANSFECTION; IN VITRO STUDY; IN VIVO STUDY; MITOSIS INHIBITION; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN PROTEIN INTERACTION; REACTION ANALYSIS;

1-PHOSPHATIDYLINOSITOL 3-KINASE; 3T3 CELLS; ANIMALS; ANTIMETABOLITES; BLOTTING, WESTERN; BROMODEOXYURIDINE; CELL CYCLE; CELL LINE; CELL NUCLEUS; CYCLIN E; CYCLINS; CYTOPLASM; DNA, COMPLEMENTARY; FLOW CYTOMETRY; G0 PHASE; G1 PHASE; GLUTATHIONE TRANSFERASE; GREEN FLUORESCENT PROTEINS; HUMANS; LUCIFERASES; LUMINESCENT PROTEINS; MICE; MICROSCOPY, FLUORESCENCE; PLASMIDS; PRECIPITIN TESTS; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RETINOBLASTOMA PROTEIN; TIME FACTORS; TRANSFECTION; TUMOR CELLS, CULTURED;

ANIMALIA;

EID: 0037369896     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.23.5.1717-1725.2003     Document Type: Article

References (27)

1. Antonetti, D. A., P, Algenstaedt, and C. R. Kahn. 1996. Insulin receptor substrate 1 binds two novel splice variants of the regulatory subunit of phosphatidylinositol 3-kinase in muscle and brain. Mol. Cell. Biol. 16:2195-2203.
2. Bacus, S. S., A. V. Gudkov, C. R. Zelnick, D. Chin, R. Stern, I. Stancovski, E. Peles, N. Ben-Baruch, H. Farbstein, R. Lupu, D. Wen, M. Sela, and Y. Yarden. 1993. Neu differentiation factor (heregulin) induces expression of intercellular adhesion molecule 1: implications for mammary tumors. Cancer Res. 53:5251-5261.
3. Bondeva, T., L. Pirola, G. Bulgarelli-Leva, I. Rubio, R. Wetzker, and M. P. Wymann. 1998. Bifurcation of lipid and protein kinase signals of PI-3K to the protein kinases PKB and MAPK. Science 282:293-296.
4. Brehm, A., E. A. Miska, D. J. McCance, J. L. Reid, A. J. Bannister, and T. Kouzarides. 1998. Retinoblastoma protein recruits histone deacetylase to repress transcription. Nature 391:597-601.
5. Brennan, P., J. W. Babbage, B. M. T. Burgering, B. Gronder, K. Reif, and D. A. Cantrell. 1997. Phosphatidylinositol 3-kinase couples the interleukin-2 receptor to the cell cycle regulator E2F. Immunity 7:679-689.
6. S6k integrates phosphatidylinositol 3-kinase and rapamycin-regulated signals for E2F regulation in T lymphocytes. Mol. Cell. Biol. 19:4729-4738.
7. Downward, J. 1998. Lipid-regulated kinases: some common theme at last. Science 279:673-674.
8. Fruman, D. A., R. E. Meyers, and L. C. Cantley. 1998. Phosphoinositide kinases. Annu. Rev. Biochem. 67:481-507.
9. 1 restriction point regulation in Rb-deficient fibroblasts. Mol. Cell. Biol. 16:2402-2407.
10. Hiebert, S. W., S. P. Chellappan, J. M. Horowitz, and J. R. Nevins. 1992, The interaction of RB with E2F coincides with an inhibition of the transcriptional activity of E2F. Genes Dev. 6:177-185.
11. Inukai, K., M. E. Anai, T. Van Breda, H. Hosaka, M. Katagiri, Y. Funaki, T. Fukushima, Y. Ogihara, M. Yazaki, Y. Kikuchi, Y. Oka, and T. Asano. 1996. A novel 55KDa regulatory subunit for phosphatidyl inositol 3-kinase structurally similar to p55γ is generated by alternative splicing of the p85α gene. J. Biol. Chem. 271:5317-5320.
12. Kaelin, W. G. 1999. Functions of the retinoblastoma proteins. Bioessays 21:950-958.
13. Kraus, M. H., N. C. Popescu, S. C. Amsbaugh, and C. R. King. 1987. Overexpression of the EGF receptor-related proto-oncogene ErbB-2 in human mammary tumor cell lines by different molecular mechanisms. EMBO J. 6:605-610.
14. Pons, S., T. Osano, E. Glasheen, M. Miralpeix, Y. Zhang, T. L. Fisher, M. G. Myers, X.-J. Sun, and M. White. 1995. The structure and function of p55γ reveals a new regulatory subunit for phosphatidylinositol 3-kinase. Mol. Cell. Biol. 15:4453-4465.
15. Ramsby, M. L., and G. S. Makowski. 1997. Differential detergent fractionation of eukaryotic cells. Methods Mol. Biol. 112:53-67.
16. Rodriquez-Viciana, P., P. H. Wanre, B. Vanhaesebroeck, and M. D. Waterfield. 1996. Activation of phosphoinositide 3-kinase by interaction with Ras and by point mutants. EMBO J. 15:2442-2451.
17. Sellers, W. R., and W. Kaelin. 1996. Rb as a modulator of transcription. Biochim. Biophys. Acta 1288:M1-M5.
18. 1-phase progression. Genes Dev. 13:1501-1512.
19. Stephens, L. R., T. R. Jackson, and P. T. Hawkins. 1993. Agonist-stimulated synthesis of phosphatidylinositol (3,4,5)-triphosphate: a new intracellular signaling system? Biochim. Biophys. Acta 1179:27-75.
20. S6K-independent signaling in growth factor-stimulated NIH 3T3 fibroblasts. Mol. Cell. Biol. 19:1346-1358.
21. Vanhaesebroeck, B., S. J. Leevers, G. Panayotou, and M. D. Waterfield. 1997. Phosphoinositide 3-kinases: a conserved family of signal transducers. Trends Biochem. Sci. 22:267-272.
22. Watanabe, G., C. Albanese, R. J. Lee, R. J. A. Reutens, G. Vairo, B. Henglein, and R. G. Pestell. 1998. Inhibition of cyclin DI kinase activity is associated with E2F-mediated inhibition of cyclin D1 promoter activity through E2F and Spl. Mol. Cell. Biol. 18:3212-3222.
23. Weinberg, R. A. 1995. The retinoblastoma protein and cell cycle control. Cell 81:323-330.
24. Xia, X., and G. Serrero. 1999. Multiple forms of p55, a regulatory subunit of phosphoinositide 3-kinase, are generated by alternative initiation of translation. Biochem. J. 341:831-837.
25. Xia, X., A. Cheng, T. Lessor, Y. Zhang, and A. W. Hamburger. 2001. Ebp1, an ErbB-3 binding protein, interacts with Rb and affects Rb transcriptional regulation. J. Cell. Physiol. 187:209-217.
26. Zacksenhaus, E., Z. Jiang, D. Chung, J. D. Marth, R. A. Phillips, and B. L. Gallie. 1996. pRb controls proliferation, differentiation and death of skeletal muscle cells and other lineages during embryogenesis. Genes Dev. 10:3051-3064.
27. 1/S cyclin-dependent kinases. J. Biol. Chem. 272:12738-12746.