Generation of a functional monomolecular protein lattice consisting of an S-layer fusion protein comprising the variable domain of a camel heavy chain antibody

Bioconjugate Chemistry

Volumn 14, Issue 2, 2003, Pages 440-448

  Pleschberger, Magdalena ^a,b   Neubauer, Angela ^b   Egelseer, Eva M ^a,b   Weigert, Stefan ^b   Lindner, Brigitte ^b   Sleytr, Uwe B ^a   Muyldermans, Serge ^c   Sára, Margit ^a,b  

^a BMT Biomolecular Therapeutics GmbH   (Austria)

^b UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

^c VRIJE UNIVERSITEIT BRUSSEL   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODIES; BACTERIA; BACTERIOLOGY; CELL MEMBRANES; CHAINS; ENCODING (SYMBOLS); ENZYMES; SELF ASSEMBLY; SIGNAL ENCODING; SURFACE PLASMON RESONANCE;

FUNCTIONAL PROTEINS; FUSION PROTEINS; HEAVY-CHAIN ANTIBODIES; MONOMOLECULARS; PROTEIN LATTICE; SECONDARY CELL WALL POLYMERS; SEQUENCE ENCODING; SOLID SUPPORTS; SURFACE LAYERS; VARIABLE DOMAIN;

ANTIGENS;

ANTIGEN; CHIMERIC PROTEIN; CRYSTALLIN; GOLD; HYBRID PROTEIN; IMMUNOGLOBULIN HEAVY CHAIN; LYSOZYME; PEPTIDOGLYCAN; POLYMER; POLYSTYRENE; PROTEIN; PROTEIN SBPA; THIOL DERIVATIVE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; BACILLUS SPHAERICUS; BACTERIAL CELL WALL; BIOTECHNOLOGY; CAMEL; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; CRYSTALLIZATION; MATERIAL COATING; NANOTECHNOLOGY; NONHUMAN; PROTEIN ANALYSIS; PROTEIN ASSEMBLY; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN STRUCTURE; SOLID; SOLUBILITY; SURFACE PLASMON RESONANCE;

BACILLUS SPHAERICUS; BACTERIA (MICROORGANISMS); CAMELIDAE; POSIBACTERIA;

EID: 0037345804     PISSN: 10431802     EISSN: None     Source Type: Journal    
DOI: 10.1021/bc025603+     Document Type: Article

References (41)

1. Sára, M., and Sleytr, U. B. (2000) S-Layer proteins. J. Bacteriol. 182, 859-868.
2. Sleytr, U. B., and Beveridge, T. J. (1999) Bacterial S-layers. Trends Microbiol. 7, 253-260.
3. Sleytr, U. B., Messner, P., Pum, D., and Sára, M. (1999) Crystalline bacterial cell surface layers (S-layers): from supramolecular cell structure to biomimetics and nanotechnology. Angew. Chem., Int. Ed. 38, 1034-1054.
4. Brechtel, E., and Bahl, H. (1999) In Thermoanaerobacterium thermosulfurigenes EM1 S-layer homology domains do not attach to peptidoglycan. J. Bacteriol. 181, 5017-5023.
5. Chauvaux, S., Matuschek, M., and Beguin, P. (1999) Distinct affinity of binding sites for S-layer homologous domains in Clostridium thermocellum and Bacillus anthracis cell envelopes. J. Bacteriol. 181, 2455-2458.
6. Lemaire, M., Miras, I., Gounon, P., and Beguin, P. (1998) Identification of a region responsible for binding to the cell wall within the S-layer protein of Clostridium thermocellum. Microbiology 144, 211-217.
7. Mesnage, S., Fontaine, T., Mignot, T., Delepierre, M., Mock, M., and Fouet, A. (2000) Bacterial SLH domain proteins are noncovalently anchored to the cell surface via a conserved mechanism involving wall polysaccharide pyruvylation. EMBO J. 19, 4473-4484.
8. Sára, M. (2001) Conserved anchoring mechanisms between crystalline cell surface S-layer proteins and secondary cell wall polymers in Gram-positive bacteria? Trends Microbiol. 9, 47-49.
9. Pum, D., Neubauer, A., Györvary, E., Sára, M., and Sleytr, U. B. (2000) S-layer proteins as basic building blocks in a biomolecular constructionkit. Nanotechnology 11, 100-107.
10. Sleytr, U. B., Sára, M., and Pum, D. (2000) Crystalline bacterial cell surface layers (S-layers): a versatile self- assembly system. In Supramolecular Polymers (A. Ciferri, Ed.) pp 177-213, Marcel Dekker, Inc., New York - Basel.
11. Sleytr, U. B., Sára, M., Pum, D., and Schuster, B. (2002) Molecular nanotechnology and nanobiotechnology with two- dimensional protein crystals (S-layers). In Nano-Surface Chemistry (M. Rosoff, Ed.) pp 333-389, Marcel Dekker, Inc., New York - Basel.
12. Ilk, N., Völlenkle, C., Egelseer, E. M., Breitwieser, A., Sleytr, U. B., and Sára, M. (2002) Molecular Characterization of the S-Layer Gene, sbpA, of Bacillus sphaericus CCM 2177 and Production of a Functional S-Layer Fusion Protein with the Ability To Recrystallize in a Defined Orientation while Presenting the Fused Allergen. Appl. Environ. Microbiol. 68, 3251-3260.
13. Ilk, N., Kosma, P., Puchberger, M., Egelseer, E. M., Mayer, H. F., Sleytr, U. B., and Sára, M. (1999) Structural and functional analyses of the secondary cell wall polymer of Bacillus sphaericus CCM 2177 that serves as an S-layer-specific anchor. J. Bacteriol. 181, 7643-7646.
14. Muyldermans, S. (2001) Single domain camel antibodies: current status. Mol. Biotechnol. 74, 277-302.
15. Nguyen, V. K., Desmyter, A., and Muyldermans, S. (2001) Functional heavy-chain antibodies in Camelidae. Adv. Immunol. 79, 261-296.
16. Desmyter, A., Transue, T. R., Ghahroudi, M. A., Thi, M. H., Poortmans, F., Hamers, R., Muyldermans, S., and Wyns, L. (1996) Crystal structure of a camel single-domain VH antibody fragment in complex with lysozyme. Nat. Struct. Biol. 3, 803-811.
17. Sheriff, S., Silverton, E. W., Padlan, E. A., Cohen, G. H., Smith-Gill, S. J., Finzel, B. C., and Davies, D. R. (1987) Three-dimensional structure of an antibody-antigen complex. Proc. Natl. Acad. Sci. U.S.A. 84, 8075-8079.
18. Padlan, E. A., Silverton, E. W., Sheriff, S., Cohen, G. H., Smith-Gill, S. J., and Davies, D. R. (1989) Structure of an antibody-antigen complex: crystal structure of the HyHEL-10 Fab-lysozyme complex. Proc. Natl. Acad. Sci. U.S.A. 86, 5938-5942.
19. Amit, A. G., Mariuzza, R. A., Phillips, S. E., and Poljak, R. J. (1986) Three-dimensional structure of an antigen-antibody complex at 2.8 A resolution. Science 233, 747-753.
20. Bhat, T. N., Bentley, G. A., Boulot, G., Greene, M. I., Tello, D., Dall'Acqua, W., Souchon, H., Schwarz, F. P., Mariuzza, R. A., and Poljak, R. J. (1994) Bound water molecules and conformational stabilization help mediate an antigen-antibody association. Proc. Natl. Acad. Sci. U.S.A. 91, 1089-1093.
21. Chitarra, V., Alzari, P. M., Bentley, G. A., Bhat, T. N., Eisele, J. L., Houdusse, A., Lescar, J., Souchon, H., and Poljak, R. J. (1993) Three-dimensional structure of a heteroclitic antigen-antibody cross-reaction complex. Proc. Natl. Acad. Sci. U.S.A. 90, 7711-7715.
22. Braden, B. C., Souchon, H., Eisele, J. L., Bentley, G.A., Bhat, T. N., Navaza, J., and Poljak, R. J. (1994) Three- dimensional structures of the free and the antigen-complexed Fab from monoclonal anti-lysozyme antibody D44.1. J. Mol. Biol. 243, 767-781.
23. Davies, D. R., Padlan, E. A., and Sheriff, S. (1990) Antibody-antigen complexes. Annu. Rev. Biochem. 59, 439-473.
24. Lescar, J., Pellegrini, M., Souchon, H., Tello, D., Poljak, R. J., Peterson, N., Greene, M., and Alzari, P. M. (1995) Crystal structure of a cross-reaction complex between Fab F9.13.7 and guinea fowl lysozyme. J. Biol. Chem. 270, 18067-18076.
25. Hoogenboom, H. R., Griffiths, A. D., Johnson, K. S., Chiswell, D. J., Hudson, P., and Winter, G. (1991) Multi-subunit proteins on the surface of filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains. Nucleic. Acids Res. 19, 4133-4137.
26. Winter, G., Griffiths, A. D., Hawkins, R. E., and Hoogenboom, H. R. (1994) Making antibodies by phage display technology. Annu. Rev. Immunol. 12, 433-455.
27. Jarosch, M., Egelseer, E. M., Mattanovich, D., Sleytr, U. B., and Sára, M. (2000) S-layer gene sbsC of Bacillus stearothermophilus ATCC 12980: molecular characterization and heterologous expression in Escherichia coli. Microbiology 146, 273-281.
28. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
29. Messner P., Hollaus F., and Sleytr, U. B. (1984) Paracrystalline cell wall surface layers of different Bacillus stearothermophilus strains. Int. J. Syst. Bacteriol. 34, 202-210.
30. Jarosch, M., Egelseer, E. M., Huber, C., Moll, D., Mattanovich, D., Sleytr, U. B., and Sára, M. (2001) Analysis of the structure-function relationship of the S-layer protein SbsC of Bacillus stearothermophilus ATCC 12980 by producing truncated forms. Microbiology 147, 1353-1363.
31. Egelseer, E. M., Schocher, I., Sleytr, U. B., and Sára, M. (1996) Evidence that an N-terminal S-layer protein fragment triggers the release of a cell-associated high-molecular-weight amylase in Bacillus stearothermophilus ATCC 12980. J. Bacteriol. 178, 5602-5609.
32. Breitwieser, A., Küpcü, S., Howorka, S., Weigert, S., Langer, C., Hoffmann-Sommergruber, K., Scheiner, O., Sleytr, U. B., and Sára, M. (1996) 2-D protein crystals as an immobilization matrix for producing reaction zones in dipstick-style immunoassays. Biotechniques 21, 918-925.
33. Breitwieser, A., Mader, C., Schocher, I., Hoffmann-Sommergruber, K., Aberer, W., Scheiner, O., Sleytr, U. B., and Sára, M. (1998) A novel dipstick developed for rapid Bet v 1-specific IgE detection: recombinant allergen immobilized via a monoclonal antibody to crystalline bacterial cell-surface layers. Allergy 53, 786-793.
34. Weiner C., Sára M., and Sleytr UB. (1994) Novel protein A affinity matrix prepared from two-dimensional protein crystals. Biotechnol. Bioeng. 43, 321-330.
35. Mader, C., Moll, D., Hotzy, C., and Sára, M. (2002) Realtime monitoring of the interaction between a cristalline bacterial cell surface (S-) and a secondary cell wall polymer serving as attachment site in the cell wall sacculus using surface plasmon resonance biosensor technology. Unpublished results.
36. Butt, H.-J. (1991) Measuring electrostatic, van der Waals, and hydration forces in electrolyte solutions with an atomic force microscope. Biophys. J. 60, 1438-1444.
37. Butt, H.-J. (1992) Measuring local surface charge densities in electrolyte solutions with a scanning force microscope. Biophys. J. 63, 578-582.
38. Kelley, T. W., Schorr, P. A., Johnson, K. D., Tirrell, M., and Frisbie, C. D. (1998) Direct Force Measurements at Polymer Brush Surfaces by Atomic Force Microscopy. Macromolecules 31, 4297-4300.
39. Overney, R. M., Leta, D. P., Pictroski, C. F., Rafailovich, M., Liu, Y., Quinn, J., Sokolov, J., Eisenberg, A., and Overney, G. (1996) Compliance measurements of confined polystyrene solutions by atomic force microscopy. Phys. Rev. Lett. 76, 1272-1275.
40. Abendroth, R. P. (1970) Behaviour of pyrogenic silica in simple electrolytes. J. Colloid Interface Sci. 34, 591-596.
41. Schmidt, T. G., and Skerra, A. (1994) One-step affinity purification of bacterially produced proteins by means of the "Strep tag" and immobilized recombinant core streptavidin. J. Chromatogr. A 676, 337-345.