2-D protein crystals as an immobilization matrix for producing reaction zones in dipstick-style immunoassays

BioTechniques

Volumn 21, Issue 5, 1996, Pages 918-925

  Breitwieser, A ^a   Kupcu S ^a   Howorka, S ^a   Weigert, S ^a   Langer, C ^a   Hoffmann Sommergruber, K ^b   Scheiner, O ^b   Sleytr, U B ^a   Sara M ^a  

^a UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

^b Inst F Allg Experimentelle P   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; GLUTARALDEHYDE; IMMUNOGLOBULIN G; POLLEN ANTIGEN; PROTEIN A; STREPTAVIDIN;

ANTIBODY DETECTION; ARTICLE; BACILLUS SPHAERICUS; BACTERIAL CELL WALL; CELL SURFACE; CONTROLLED STUDY; CRYSTAL STRUCTURE; HUMAN; IMMUNOASSAY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN CROSS LINKING; PROTEIN IMMOBILIZATION; ULTRAFILTRATION;

BACILLUS SPHAERICUS; BACTERIA (MICROORGANISMS);

EID: 0029837475     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96215rr05     Document Type: Article

References (19)

1. Bamford, C.H., K.G. Al-Lamee, M.D. Purbrick and T.J. Wear. 1992. Studies of a novel membrane for affinity separation. J. Chromatogr. 606:19-31.
2. Beveridge, T.J. and S.F. Koval. 1993. Advances in bacterial paracrystalline surface layers. Plenum Press, New York.
3. Björk, I., B-Å Petersson and J. Sjöquist. 1972. Some physiochemieal properties of Protein A from Staphylococcus aureus. Eur. J. Biochem. 29:579-584.
4. Breiteneder, H., K. Pettenburger, A. Bito, D. Kraft, H. Rumpold, O. Scheiner and M. Breitenbach. 1989. The gene coding for the major birch allergen, Bet v1, is highly homologous to a pea disease resistance response gene. EMBO J. 8:1935.
5. Champulvier, B. and M.R. Kula. 1991. Microfiltration membranes as pseudo-affinity adsorbents: modification and comparison with gel beads. J. Chromatogr. 539:315-325.
6. Chase, H.A. 1983. Affinity separations utilising immobilised monoclonal antibodies - a new tool for the biochemical engineer. Chem. Eng. Sci. 39:1099-1125.
7. Green, N.M. 1965. A spectrophotometric assay for avidin and biotin based on binding dyes by avidin. Biochem. J. 94:23c-24c.
8. Jarolim, E., M. Tejkl, M. Rohac, G. Schlerka, O. Scheiner, D. Kraft, M. Breitenbach and H. Rumpold. 1989. Monoclonal antibodies against birch pollen allergen: characterisation by immunoblotting and use for single step affinity purification of the major allergen Bet v1. Int. Arch. Allergy Appl. Immunol. 90:54.
9. Kim, M., K. Saito, S. Furusaki, T. Sugo and I. Ishigaki. 1991. Protein adsorption capacity of a porous phenylalanine-containing membrane based on a polyethylene matrix. J. Chromatogr. 586:27-33.
10. Langone, J.J. 1982. Protein A of Staphylococcus aureus and related immunoglobulin receptors produced by Streptococci and Pneumococci. Adv. Immunol. 32:157-252.
11. Lowry, O.H., N.J. Rosebrough, A.L. Karr and R.J. Randall. 1951. Protein measurement with Folin-phenol reagent. J. Biol. Chem. 193:265-275.
12. Messner, P., F. Hollaus and U.B. Sleytr. 1984. Paracrystalline cell wall surface layers of different Bacillus stearothermaphilus strains. Int. J. Syst. Bacteriol. 34:202-210.
13. Messner, P. and U.B. Sleytr. 1992. Crystalline bacterial cell surface layers. Adv. Microbiol. Physiol. 33:213-275.
14. Sára, M. and U.B. Sleytr. 1987. Production and characteristics of ultrafiltration membranes with uniform pores from two-dimensional arrays of proteins. J. Membr. Sci. 33:27-47.
15. Sára, M., S. Küpcü, C. Weiner, S. Weigert and U.B. Sleytr. 1993. Crystalline protein layers as isoporous molecular sieves and immobilisation and affinity matrices, p. 71-86. In U.B. Sleytr, P. Messner, D. Pum and M. Sára (Eds.), Immobilised Macromolecules: Application Potentials. Springer, London.
16. Sleytr, U.B., P. Messner, D. Pum and M. Sára (Eds.). 1996. Crystalline Bacterial Cell Surface Proteins. Landes Company, Academic Press, Austin.
17. Smith, P.K., R.I. Krohn, G.T. Hermanson, A.K. Mallia, F.H. Gartner, M.D. Provenzano, E.K. Fujimoto, N.M. Goeke, B.J. Olson and D.C. Klenk. 1985. Measurement of protein using bicinchonic acid. Anal. Biochem. 150:76-85.
18. Weigert, S. and M. Sára. 1995. Surface modification of an ultrafiltration membrane with crystalline structure and studies on interactions with selected protein molecules, J. Membr. Sci. 106:147-159.
19. Weiner, C., M. Sára and U.B. Sleytr. 1993. Novel Protein A affinity matrix prepared from two-dimensional protein crystals. Biotechnol. Bioeng. 44:321-330.