In Thermoanaerobacterium thermosulfurigenes EM1 S-layer homology domains do not attach to peptidoglycan

Journal of Bacteriology

Volumn 181, Issue 16, 1999, Pages 5017-5023

  Brechtel, Elke ^a   Bahl, Hubert ^a  

^a UNIVERSITY OF ROSTOCK   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; CELL SURFACE PROTEIN; PEPTIDOGLYCAN; XYLAN ENDO 1,3 BETA XYLOSIDASE;

ANAEROBIC BACTERIUM; ARTICLE; BACTERIAL CELL WALL; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION; THERMOPHILIC BACTERIUM;

EID: 0032839730     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.181.16.5017-5023.1999     Document Type: Article

References (32)

1. Antranikian, G. 1990. Physiology and enzymology of thermophilic anaerobic bacteria degrading starch. FEMS Microbtol. Rev. 75:201-218.
2. Antranikian, G., C. Herzberg, and G. Gottschalk. 1987. Production of ther-mostable a-amylase, pullulanase, and α-glucosidase in continuous culture by a new Clostridium isolate. Appl. Environ. Microbiol. 53:1668-1673.
3. Bergmejer, H. U., K. Gawehn, and M. Grabe. 1974. Enzyme als biochemische Reagenzien, p. 454-558. In H. U. Bergmeyer (ed.), Methoden der enzymatischen Analyse. Verlag Chemie, Weinheim, Germany.
4. Birnboim, H. C., and J. Doly. 1979. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7:1513-1523.
5. Blura, H., H. Beier, and H. J. Gross. 1987. Improved silver staining method of plant proteins, RNA and DNA in poryacrylamide gels. Electrophoresis 8:93-99.
6. Bradford, M. M. 1976. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
7. Brechtel, E., M. Matuschek, A. Hellberg, E. M. Egelseer, R. Schmid. and H. Bahl. 1999. Cell wall of Thermoanaerobacterium themosulfurigenes EM1: isolation of its components and attachment of the xylanase XynA. Arch. Microbiol. 171:159-165.
8. Edge, A. S. B., C. R. Faltynek, L. Hof, L. E. Reichert, Jr., and P. Weber. 1981. Deglycosylation of glycoproteins by trifluoromethanesulfonic acid. Anal. Biochem. 118:131-137.
9. Egelseer, E. M., K. Leitner, M. Jarosch, C. Hotzy, S. Zayni, U. B. Sleytr, and M. Sára. 1998. The S-layer proteins of two Bacillus stearothermophitus wildtype strains are bound via their N-terminal region to a secondary cell wall polymer of identical chemical composition. J. Bacteriol. 180:1488-1495.
10. Egelseer, E. M., I. Schocher, M. Sara, and U. B. Sleytr. 1995. The S-layer from Bacillus stearothermophilus DSM 2358 functions as an adhesion site for a high-molecular-weight amylase. J. Bacteriol. 177:1444-1451.
11. Engel, A., Z. Cejka, A. Lupas, F. Lottspeich, and W. Baumelster. 1992. Isolation and cloning of Ompα, a coiled-coil protein spanning the periplasmic space of the ancestral eubacterium Thermotoga maritima. EMBO J. 11:4369-4378.
12. Hastie, A. T., and C. C. Brinton, Jr. 1979. Specific interaction of the tetragonally arrayed protein layer of Bacillus sphaericus with its peptidoglycan sacculus. J. Bacteriol. 138:1010-1021.
13. Kozianowski, G. 1988. Charakterisierung der Zellwand von Clostridium thermosulfurogenes EM1 und Einfluß der Wachstumsbedingungen auf die Zellwandzusammensetzung. Diploma thesis. University of Göttingen, Göttingen, Germany.
14. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
15. Lemaire, M., I. Miras, P. Gounon, and P. Béguin. 1998. Identification of a region responsible for binding to the cell wall within the S-layer protein of Clostridium thermoceUum. Microbiology 144:211-217.
16. Lemaire, M., H. Ohayon, P. Gounon, T. Fujino, and P. Béguin. 1995. OlpB, a new outer layer protein of Clostridium thermocellum, and binding of its S-layer-like domains to components of the cell envelope. J. Bacteriol. 177: 2451-2459.
17. Lupas, A., H. Engelhardt, J. Peters, U. Santarius, S. Volker, and W. Baumeister, 1994. Domain structure of the Acetogmium kivui surface layer revealed by electron crystallography and sequence analysis. J. Bacteriol. 176:1224-1233.
18. Mantle, M., and A. Allen. 1978. A colorimetric assay for glycoproteins based on the periodic acid/Schiff stain. Biochem. Soc. Trans. 6:607-609.
19. Masuda, K., and T. Kawata. 1981. Characterization of a regular array in the wall of Lactobacillus buchneri and its reattachment to the other wall components. J. Gen. Microbiol. 124:81-90.
20. Matuschek, M., G. Burchhardt, K. Sahm, and H. Bahl. 1994. Pullulanase of Thermoanaerobacterium thermosulfurigenes EM1 (Clostridium thermosulfurigenes): molecular analysis of the gene, composite structure of the enzyme, and a common model for its attachment to the cell surface. J. Bacteriol. 176:3295-3302.
21. Matuschek, M., K. Sahm, A. Zibat, and H. Bahl. 1996. Characterization of genes from Thermoanaerobacterium thermosulfurigenes EM1 that encode two glycosyl hydrolases with conserved S-layer-like domains. Mol. Gen. Genet. 252:493-496.
22. Messner, P., and U. B. Sleytr. 1988. Separation and purification of S-layers from Gram-positive and Gram-negative bacteria, p. 97-104. In I. C. Hancock and I. R. Poxton (ed.), Bacterial cell surface techniques. Wiley & Sons, Chichester, Great Britain.
23. Olabarría, G., J. L. Carrascosa, M. A. de Pedro, and J. Berenguer. 1996. A conserved motif in S-layer proteins is involved in peptidoglycan binding in Thermus themophilus. J. Bacteriol. 178:4765-4772.
24. Ries, W., C. Hotzy, 1. Schocher, U. B. Sleytr, and M. Sára. 1997. Evidence that the N-terminal part of the S-layer protein from Bacillus stearothermophilus PV72/p2 recognizes a secondary cell wall polymer. J. Bacteriol. 179:38923898.
25. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N. Y.
26. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
27. Sára, M., B. Kuen, H. F. Mayer, F. Mandl, K. C. Schuster, and U. B. Sleytr. 1996. Dynamics in oxygen-induced changes in S-layer protein synthesis from Bacillus stearothermophilus PV72 and the S-layer-deficient variant T5 in continuous culture and studies of the cell wall composition. J. Bacteriol. 178:2108-2117.
28. Schwarz, W. H., K. Bronnenmeier, F. Grabnitz. and W. L. Staudenbauer. 1987. Activity staining of cellulases in polyacrylamide gels containing mixed linkage beta-glucans. Anal. Biochem. 164:72-77.
29. Specka, U., A. Spreinat, G. Antranikian, and F. Mayer. 1991. Immunocyto-chemical identification and localization of active and inactive α-amylase and pullulanase in cells of Clostridium themosulfuroneges EM1, Appl. Environ. Microbiol. 57:1062-1069.
30. Spreinat, A., and G. Antranikian. 1992. Analysis of the amylolytic enzyme system of Clostridium thermosulfurogenes EM1: purification and synergistic action of pullulanases and maltohexaose forming α-amylase. Starch/stärke 44:305-312.
31. Studier, F. W., and B. A. Moffat. 1986. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189:113-130.
32. Wong, K. K. Y., L. U. L. Tan, and J. N. Saddler. 1988. Multiplicity of β-1,4-xylanases in microorganisms: functions and applications. Microbiol. Rev. 52:305-317.