A non-proteolytic function of separase links the onset of anaphase to mitotic exit

Nature Cell Biology

Volumn 5, Issue 3, 2003, Pages 249-254

  Sullivan, Matt ^a   Uhlmann, Frank ^a  

^a THE FRANCIS CRICK INSTITUTE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CHROMOSOME PROTEIN; COHESIN; CYCLIN DEPENDENT KINASE; ENZYME INHIBITOR; FUNGAL ENZYME; PHOSPHATASE; PROTEIN KINASE; PROTEINASE; SECURININE; SEPARASE; UNCLASSIFIED DRUG;

ANAPHASE; ARTICLE; CELL ADHESION; CELL BUDDING; CELL CYCLE G1 PHASE; CHROMOSOME SEGREGATION; CONTROLLED STUDY; CYTOKINESIS; DOWN REGULATION; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME PHOSPHORYLATION; MITOSIS; NONHUMAN; NUCLEOLUS; PRIORITY JOURNAL; PROTEIN DEGRADATION; REGULATORY MECHANISM; SISTER CHROMATID; YEAST CELL;

ANAPHASE; CELL CYCLE PROTEINS; CELL NUCLEOLUS; ENDOPEPTIDASES; HYDROLYSIS; MITOSIS; PHOSPHORYLATION; PROTEIN-TYROSINE-PHOSPHATASE; SACCHAROMYCES CEREVISIAE PROTEINS;

SACCHAROMYCETALES;

EID: 0037340260     PISSN: 14657392     EISSN: None     Source Type: Journal    
DOI: 10.1038/ncb940     Document Type: Article

References (33)

1. Uhlmann, F., Wernic, D., Poupart, M.-A., Koonin, E. V. & Nasmyth, K. Cleavage of cohesin by the CD clan protease separin triggers anaphase in yeast. Cell 103, 375-386 (2000).
2. Nasmyth, K. Segregating sister genomes: the molecular biology of chromosome separation. Science 297, 559-565 (2002).
3. Visintin, R., Hwang, E. S. & Amon, A. Cfi1 prevents premature exit from mitosis by anchoring Cdc14 phosphatase in the nucleolus. Nature 398, 818-823 (1999).
4. Shou, W. et al. Exit from mitosis is triggered by Tem1-dependent release of the protein phosphatase Cdc14 from nucleolar RENT complex. Cell 97, 233-244 (1999).
5. Bardin, A. J. & Amon, A. MEN and SIN: what's the difference? Nature Rev. Mol. Cell Biol. 2, 1-12 (2001).
6. Tinker-Kulberg, R. L. & Morgan, D. O. Pds1 and Esp1 control both anaphase and mitotic exit in normal cells and after DNA damage. Genes Dev. 13, 1936-1949 (1999).
7. Granot, D. & Snyder, M. Segregation of the nucleolus during mitosis in budding and fission yeast. Cell Motil. Cytoskeleton 20, 47-54 (1991).
8. Yeong, F. M., Lim, H. H., Wang, Y. & Surana, U. Early expressed Clb proteins allow accumulation of mitotic cyclin by inactivating proteolytic machinery during S phase. Mol. Cell. Biol. 21, 5071-5081 (2001).
9. Cdc20 promotes exit from mitosis by destroying the anaphase inhibitor Pds1 and cyclin Clb5. Nature 402, 203-207 (1999).
10. Yeong, F. M., Lim, H. H., Padmashree, C. G. & Surana, U. Exit from mitosis in budding yeast: Biphasic inactivation of the Cdc28-Clb2 mitotic kinase and the role of Cdc20. Mol. Cell 5, 501-511 (2000).
11. Stegmeier, F., Visintin, R. & Amon, A. Separase, polo kinase, the kinetochore protein Slk19, and Spo12 function in a network that controls Cdc14 localization during early anaphase. Cell 108, 207-220 (2002).
12. Jaspersen, S. L., Charles, J. F., Tinker-Kulberg, R. L. & Morgan, D. O. A late mitotic regulatory network controlling cyclin destruction in Saccharomyces cerevisiae. Mol. Biol. Cell 9, 2803-2817 (1998).
13. Lee, S. E., Frenz, L. M., Wells, N. J., Johnson, A.L. & Johnston, L. H. Order of function of the budding yeast mitotic exit-network proteins Tem1, Cdc15, Mob1, Dbf2, and Cdc5. Curr. Biol. 11, 784-788 (2001).
14. Shou, W. et al. Cdc5 influences phosphorylation of Net1 and disassembly of the RENT complex. BMC Mol. Biol. 3, 3 (2002).
15. Yoshida, S. & Toh-e, A. Budding yeast Cdc5 phosphorylates Net1 and assists Cdc14 release from the nucleolus. Biochem. Biophys. Res. Commun. 294, 687-691 (2002).
16. Sullivan, M., Lehane, C. & Uhlmann, F. Orchestrating anaphase and mitotic exit: separase cleavage and localization of Slk19. Nature Cell Biol. 3, 771-777 (2001).
17. Baum, P., Yip, C., Goetsch, L. & Byers, B. A yeast gene essential for regulation of spindle pole duplication. Mol. Cell Biol. 8, 5386-5397 (1988).
18. Hornig, N. C. D., Knowles, P. P., McDonald, N. Q. & Uhlmann, F. The dual mechanism of separase regulation by securin. Curr. Biol. 12, 973-982 (2002).
19. Uhlmann, F., Lottspeich, F. & Nasmyth, K. Sister-chromatid separation at anaphase onset is promoted by cleavage of the cohesin subunit Scc1. Nature 400, 37-42 (1999).
20. Hu, F. et al. Regulation of the Bub2-Bfa1 GAP complex by Cdc5 and cell cycle checkpoints. Cell 107, 655-665 (2001).
21. Yoshida, S., Asakawa, K. & Toh-e, A. Mitotic exit network controls the localization of Cdc14 to the spindle pole body in Saccharomyces cerevisiae. Curr. Biol. 12, 944-950 (2002).
22. Cheng, L., Hunke, L. & Hardy, C. F. J. Cell cycle regulation of the Saccharomyces cerevisiae Polo-like kinase Cdc5p. Mol. Cell. Biol. 18, 7360-7370 (1998).
23. Juang, Y.-L. et al. APC-mediated proteolysis of Ase1 and the morphogenesis of the mitotic spindle. Science 275, 1311-1314 (1997).
24. Cohen-Fix, O. & Koshland, D. Pds1p of budding yeast has dual roles: inhibition of anaphase initiation and regulation of mitotic exit. Genes Dev. 13, 1950-1959 (1999).
25. Bembenek, J. & Yu, H. Regulation of the anaphase-promoting complex by the dual secificity phosphatase human Cdc14a. J. Biol. Chem. 276, 48237-48242 (2001).
26. Kaiser, B. K., Zimmerman, Z. A., Charbonneau, H. & Jackson, P. K. Disruption of centrosome structure, chromosome segregation, and cytokinesis by misexpression of human Cdc14A phosphatase. Mol. Biol. Cell 13, 2289-2300 (2002).
27. Bardin, A. J., Visintin, R. & Amon, A. A mechanism for coupling exit from mitosis to partitioning of the nucleus. Cell 102, 21-31 (2000).
28. Pereira, G., Höfken, T., Grindlay, J., Manson, C. & Schiebel, E. The Bub2p spindle checkpoint links nuclear migration with mitotic exit. Mol. Cell 6, 1-10 (2000).
29. Knop, M. et al. Epitope tagging of yeast genes using a PCR-based strategy: more tags and improved practical routines. Yeast 15, 963-972 (1999).
30. Wach, A., Brachat, A., Pöhlmann, R. & Philippsen, P. New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae. Yeast 10, 1793-1808 (1994).
31. Gietz, R. D. & Sugino, A. New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base restriction sites. Gene 74, 527-534 (1988).
32. Kushnirov, V. V. Rapid and reliable protein extraction from yeast. Yeast 16, 857-860 (2000).
33. Aris, J. P. & Blobel, G. Identification and characterization of a yeast nucleolar protein that is similar to a rat liver nucleolar protein. J. Cell Biol. 107, 17-31 (1988).