Phosphorylation of eIF4E attenuates its interaction with mRNA 5′ cap analogs by electrostatic repulsion: Intein-mediated protein ligation strategy to obtain phosphorylated protein

RNA

Volumn 9, Issue 1, 2003, Pages 52-61

  Zuberek, Joanna ^a   Wyslouch Cieszynska, Aleksandra ^b   Niedzwiecka, Anna ^a   Dadlez, Michal ^a,b   Stepinski, Janusz ^a   Augustyniak, Wojciech ^a   Gingras, Anne claude ^c   Zhang, Zhibo ^d   Burley, Stephen K ^e   Sonenberg, Nahum ^c   Stolarski, Ryszard ^a   Darzynkiewicz, Edward ^a  

^a UNIVERSITY OF WARSAW   (Poland)

^b INSTITUTE OF BIOCHEMISTRY AND BIOPHYSICS   (Poland)

^c MCGILL UNIVERSITY   (Canada)

^d UNIVERSITY OF TURKU   (Finland)

^e ROCKEFELLER UNIVERSITY   (United States)

Author keywords

Expressed protein ligation; Fluorescence; Intein; MRNA 5 cap; Translation initiation

Indexed keywords

CAPPED RNA; INITIATION FACTOR 4E; INTEIN; MESSENGER RNA; PHOSPHOPROTEIN; RIBONUCLEOTIDE; SERINE;

ARTICLE; COMPLEX FORMATION; CONTROLLED STUDY; FLUOROMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PHOSPHORYLATION; PROTEIN RNA BINDING; PROTEIN STABILITY; PROTEIN SYNTHESIS; TITRIMETRY; TRANSLATION INITIATION; TRANSLATION REGULATION;

ELECTROSTATICS; EUKARYOTIC INITIATION FACTOR-4E; NUCLEIC ACID CONFORMATION; PHOSPHORYLATION; RNA CAPS; RNA, MESSENGER; SPECTROMETRY, FLUORESCENCE;

EUKARYOTA;

EID: 0037273418     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: 10.1261/rna.2133403     Document Type: Article

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