Functional expression of the PHA synthase gene phaC1 from Pseudomonas aeruginosa in Escherichia coli results in poly(3-hydroxyalkanoate) synthesis

FEMS Microbiology Letters

Volumn 150, Issue 2, 1997, Pages 303-309

  Langenbach, Stephan ^a   Rehm, Bernd H A ^a   Steinbüchel, Alexander ^a  

^a UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

Escherichia coli; PHA synthase; Polyhydroxyalkanoate; Pseudomonas aeruginosa

Indexed keywords

ALKANOIC ACID; HYDROXY FATTY ACID; MEDIUM CHAIN FATTY ACID; SYNTHETASE;

ARTICLE; BACTERIAL METABOLISM; ESCHERICHIA COLI; FATTY ACID METABOLISM; GENE EXPRESSION; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA;

ACYLTRANSFERASES; BACTERIAL PROTEINS; BETA-GALACTOSIDASE; ESCHERICHIA COLI; FATTY ACIDS; HYDRO-LYASES; POLYMERS; PROMOTER REGIONS (GENETICS); PSEUDOMONAS AERUGINOSA; RECOMBINANT FUSION PROTEINS; SPECIES SPECIFICITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

EID: 0030904522     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(97)00142-0     Document Type: Article

References (15)

1. Steinbüchel, A., Hustede, E., Liebergesell, M., Pieper, U., Timm, A. and Valentin, H. (1992) Molecular basis for biosynthesis and accumulation of polyhydroxyalkanoic acids in bacteria. FEMS Microbiol. Rev. 103, 217-230.
2. Schubert, P., Steinbüchel, A. and Schlegel, H.G. (1988) Cloning of the Alcaligenes eutrophus genes for synthesis of poly-β-hydroxybutyric acid (PHB) and synthesis of PHB in Escherichia coli. J. Bacteriol. 170, 5837-5847.
3. Eggink, G., de Waard, P. and Huijberts, G.N.M. (1992) The role of fatty acid biosynthesis and degradation in the supply of substrates for poly(3-hydroxy-alkanoate) formation in Pseudomonas putida. FEMS Microbiol. Rev. 103, 159-164.
4. Steinbüchel, A. and Valentin, H.E. (1995) Diversity of bacterial polyhydroxyalkanoic acids. FEMS Microbiol. Lett. 128, 219-228.
5. 13C Nuclear magnetic resonance studies of Pseudomonas putida fatty acid metabolic routes involved in poly(3-hydroxyalkanoate) synthesis. J. Bacteriol. 176, 1661-1666.
6. Slater, S., Gallaher, T. and Dennis, D. (1992) Production of poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) in a recombinant Escherichia coli strain. Appl. Environ. Microbiol. 58, 1089-1094.
7. Timm, A. and Steinbüchel, A. (1992) Cloning and molecular characterization of the polyhydroxyalkanoic acid gene locus of Pseudomonas aeruginosa PAO1. Eur. J. Biochem. 209, 15-30.
8. Mak, Y.M. and Ho, K.K. (1991) An improved method for isolation of chromosomal DNA from various bacteria and cyanobacteria. Nucl. Acids Res. 20, 4101-4102.
9. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: a Laboratory Manual, 2nd edn., Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
10. Towbin, H., Staehelin, T. and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76, 4350-4354.
11. Timm, A., Byrom, D. and Steinbüchel, A. (1990) Formation of blends of various poly(3-hydroxyalkanoic acids) by a recombinant strain of Pseudomonas oleovorans. Appl. Microbiol. Biotechnol. 33, 296-301.
12. Cronan, Jr., J.E., Silbert, D.F. and Wulff, D.L. (1972) mapping of fabA locus for unsaturated fatty acid biosynthesis in Escherichia coli. J. Bacteriol. 112, 206-211.
13. Spratt, S.K., Ginsburgh, C.L. and Nunn, W.D. (1981) Isolation and genetic characterization of Escherichia coli mutants defective in propionate metabolism. J. Bacteriol. 146, 1166-1169.
14. DiRusso, C.C. (1990) Primary sequence of the Escherichia coli fadBA operon, encoding the fatty acid-oxidizing multienzyme complex, indicates a high degree of homology to eucaryotic enzymes. J. Bacteriol. 172, 6459-6468.
15. Spratt, S.K., Black, P.N., Ragozzino, M.M. and Nunn, W.D. (1984) Cloning, mapping, and expression of genes involved in the fatty acid-degradative multienzyme complex of Escherichia coli. J. Bacteriol. 158, 535-542.