Co-expression of yeast amber suppressor tRNA(Tyr) and Tyrosyl-tRNA synthetase in Escherichia coli: Possibility to expand the genetic code

Journal of Biochemistry

Volumn 124, Issue 6, 1998, Pages 1065-1068

  Ohno, Satoshi ^a   Yokogawa, Takashi ^a   Fujii, Isao ^a   Asahara, Haruichi ^b   Inokuchi, Hachiro ^b   Nishikawa, Kazuya ^a  

^a GIFU UNIVERSITY   (Japan)

^b KYOTO UNIVERSITY   (Japan)

Author keywords

Amber mutation; Co expression; Non canonical amino acid; Suppressor tRNA; Tyrosyl tRNA synthetase

Indexed keywords

TYROSINE; TYROSINE TRANSFER RNA; TYROSINE TRANSFER RNA LIGASE;

AMINOACYLATION; ARTICLE; BACTERIOPHAGE LAMBDA; BACTERIUM MUTANT; CONTROLLED STUDY; ESCHERICHIA COLI; FUNGUS MUTANT; GENETIC CODE; NONHUMAN; PROTEIN EXPRESSION; YEAST;

BACTERIA (MICROORGANISMS); BACTERIOPHAGE LAMBDA; ESCHERICHIA COLI; FUNGI;

EID: 0032428262     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a022221     Document Type: Article

References (15)

1. Noren, C.J., Anthony-Cahill, S.J., Griffith, M.C., and Schultz, P.G. (1989) A general method for site-specific incorporation of unnatural amino acids into protein. Science 244, 182-188
2. Hohsaka, T., Sato, K., Sisido, M., Takai, K., and Yokoyama, S. (1994) Site-specific incorporation of photofunctional nonnatural amino acids into a polypeptide through in vitro protein biosynthe-sis. FEBS Lett. 344, 171-174
3. Mendel, D., Cornish, V.W., and Schultz, P.G. (1995) Site-directed mutagenesis with an expanded genetic code. Annu. Rev. Biophys. Biomol. Struct. 24, 435-462
4. Nowak, M.W., Kearney, P.C., Sampson, J.R., Saks, M.E., Labarca, C.G., Silverman, S.K., Zhong, W., Thorson, J., Abelson, J.N., Davidson, N., Schultz, P.G., Dougherty, D.A., and Lester, H.A. (1995) Nicotinic receptor binding site probed with unnatural amino acid incorporation in intact cells. Science 268, 439-442
5. Kanamori, T., Nishikawa, S., Shin, I., Schultz, P.G., and Endo, T. (1997) Probing the environment along the protein import pathways in yeast mitochondria by site-specific photocrosslinking. Proc. Natl. Acad. Sci. USA 94, 485-490
6. Liu, D.R., Magliery, T.J., Pastrnak, M., and Schultz, P.G. (1997) Engineering a tRNA and aminoacyl-tRNA synthetase for the site-specific incorporation of unnatural amino acids into proteins in vivo. Proc. Natl. Acad. Sci. USA 94, 10092-10097
7. Furter, R. (1998) Expansion of the genetic code: Site-directed p-fluoro-phenylalanine incorporation in Escherichia coli. Protein Sci. 7, 419-426
8. Park, H. and RajBhandary, U.L. (1998) Tetracycline-regulated suppression of amber codons in mammalian cells. Mol. Cell. Biol 18, 4418-4425
9. Chow, C.M. and RajBhandary, U.L. (1993) Saccharomyces cerevisiae cytoplasmic tyrosyl-tRNA synthetase gene. J. Biol. Chem. 268, 12855-12863
10. Bare, L., Bruce, A.G., Gesteland, R., and Uhlenbeck, O.C. (1983) Uridine-33 in yeast tRNA not essential for amber suppression. Nature 305, 554-556
11. Rossi, J.J., Schuld, M., Larson, G.P., and Wallace, R.B. (1982) Functional expression of a yeast ochre suppressor tRNA gene in Escherichia coli. Gene 20, 423-432
12. +2 glutamine tRNA in E. coli. Jpn. J. Genet. 63, 237-249
13. Tyr revealed by protection studies with yeast tyrosyl-tRNA synthetase. Eur. J. Biochem. 185, 331-339
14. Pro identity. Nucleic Acids Res. 22, 522-529
15. Pro. Biochemistry 37, 8605-8613