Expression, purification and spectroscopic characterization of the cytochrome P450 CYP121 from Mycobacterium tuberculosis

Journal of Inorganic Biochemistry

Volumn 91, Issue 4, 2002, Pages 527-541

  McLean, Kirsty J ^a   Cheesman, Myles R ^b   Rivers, Stuart L ^c   Richmond, Alison ^c   Leys, David ^a   Chapman, Stephen K ^c   Reid, Graeme A ^c   Price, Nicholas C ^d   Kelly, Sharon M ^e   Clarkson, John ^d   Smith, W ^d   Munro, Andrew W ^a  

^a UNIVERSITY OF LEICESTER   (United Kingdom)

^b UNIVERSITY OF EAST ANGLIA   (United Kingdom)

^c UNIVERSITY OF EDINBURGH   (United Kingdom)

^d UNIVERSITY OF STRATHCLYDE   (United Kingdom)

^e UNIVERSITY OF GLASGOW   (United Kingdom)

Author keywords

Azole inhibitors; CYP121; Drug targets; Mycobacterium tuberculosis; P450

Indexed keywords

ANTIFUNGAL AGENT; CARBON MONOXIDE; CLOTRIMAZOLE; CYSTEINE DERIVATIVE; CYTOCHROME P450 CYP121; CYTOCHROME P450 ISOENZYME; HEME; MICONAZOLE; UNCLASSIFIED DRUG;

ABSORPTION; ARTICLE; CHROMATOGRAPHY; CONTROLLED STUDY; DRUG PROTEIN BINDING; DRUG TARGETING; ENZYME ANALYSIS; ENZYME BINDING; ENZYME CONFORMATION; ENZYME PURIFICATION; ESCHERICHIA COLI; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR CLONING; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PROTEIN EXPRESSION; SPECTROSCOPY;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; CYTOCHROME P-450 ENZYME SYSTEM; DNA PRIMERS; ELECTRON SPIN RESONANCE SPECTROSCOPY; MOLECULAR SEQUENCE DATA; MYCOBACTERIUM TUBERCULOSIS; RECOMBINANT PROTEINS; RESTRICTION MAPPING; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; SPECTROPHOTOMETRY; SPECTRUM ANALYSIS, RAMAN;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MYCOBACTERIUM; MYCOBACTERIUM TUBERCULOSIS;

EID: 0037145070     PISSN: 01620134     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0162-0134(02)00479-8     Document Type: Article

References (52)

1. Young D.B., Cole S.T. J. Bacteriol. 175:1993;1-6.
2. Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C., Harris D., Gordon S.V., Eiglmeier K., Gas S., Barry C.E., Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Conner R., Davies R., Devlin K., Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L., Oliver K., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton J., Squares R., Squares S., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature. 393:1998;537-544.
3. Blattner F.R., Plunkett G., Bloch C.A., Perna N.T., Burland V., Riley M., Collado Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science. 277:1997;1453-1474.
4. Parkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J., Churcher C., Mungall K.L., Bentley S.D., Holden M.T.G., Sebaiha M., Baker S., Basham D., Brooks K., Chillingworth T., Connerton P., Cronin A., Davis P., Davies R.M., Dowd L., White N., Farrar J., Feltwell T., Hamlin N., Haque A., Hien T.T., Holroyd S., Jagels K., Krogh A., Larsen T.S., Leather S., Moule S., O'Gaora P., Parry C., Quail M., Rutherford K., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature. 413:2001;848-852.
5. Kunst F., Ogasawara N., Moszer I., et al. Nature. 390:1997;249-256.
6. Streptomyces coelicolor genome sequence home page at the Sanger Centre: http://www.sanger.ac.uk/Projects/S_coelicolor/.
7. Mycobacterium bovis genome sequence home page at the Sanger Centre: http://www.sanger.ac.uk/Projects/M_bovis/.
8. Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C., Harris D., Mungall K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S., Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature. 409:2001;1007-1011.
9. Brennan P.J. Annu. Rev. Biochem. 64:1995;29-63.
10. Daffe M., Draper P. Adv. Microb. Physiol. 39:1998;131-203.
11. Souter A., Mclean K.J., Smith W.E., Munro A.W. J. Chem. Technol. Biotechnol. 75:2000;933-941.
12. Bellamine A., Mangla A.T., Nes W.D., Waterman M.R. Proc. Natl. Acad. Sci. USA. 96:1999;8937-8942.
13. Guardiola-Diaz H.M., Foster L.A., Mushrush D., Vaz A.N.D. Biochem. Pharmacol. 61:2001;1463-1470.
14. Nelson D.R., Koymans L., Kamataki T., Stegeman J.J., Feyereisen R., Waxman D.J., Waterman M.R., Gotoh O., Coon M.J., Estabrook R.W., Gunsalus I.C., Nebert D.W. Pharmacogenetics. 6:1996;1-42.
15. Bille J. Int. J. Antimicrob. Agents. 16:2000;331-333.
16. Okita R.T., Okita J.R. Curr. Drug Metab. 2:2001;265-281.
17. Omer C.A., Lenstra R., Litle P.J., Dean C., Tepperman J.M., Leto K.J., Romesser J.A., O'Keefe D.P. J. Bacteriol. 172:1990;3335-3345.
18. Cupp Vickery J.R., Han O., Hutchinson C.R., Poulos T.L. Nat. Struct. Biol. 3:1996;632-637.
19. Green A.J., Rivers S.L., Cheesman M.R., Reid G.A., Quaroni L.G., Macdonald I.D.G., Chapman S.K., Munro A.W. J. Biol. Inorg. Chem. 6:2001;523-533.
20. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: A Laboratory Manual. 1989;Cold Spring Harbor Laboratory Press.
21. Omura T., Sato R. J. Biol. Chem. 237:1962;1375-1376.
22. Noble M.A., Miles C.S., Chapman S.K., Lysek D.A., Mackay A.C., Reid G.A., Hanzlik R.P., Munro A.W. Biochem. J. 339:1999;371-379.
23. Sreerama N., Woody R.W. Anal. Biochem. 287:2000;252-260.
24. Vieira J. J. Messing. Gene. 19:1982;259-268.
25. Sligar S.G. Biochemistry. 15:1976;5399-5406.
26. Miles J.S., Munro A.W., Rospendowski B.N., Smith W.E., McKnight J., Thomson A.J. Biochem. J. 288:1992;503-509.
27. Hill H.A.O., Roder A., Williams R.J.P. Struct. Bonding. 8:1970;123-151.
28. Ravichandran K.G., Boddupalli S.S., Hasemann C.A., Peterson J.A., Deisenhofer J. Science. 261:1993;731-736.
29. Poulos T.L., Finzel B.C., Howard A.J. J. Mol. Biol. 195:1987;687-700.
30. Munro A.W., Lindsay J.G., Coggins J.R., Kelly S.M., Price N.C. FEBS Lett. 343:1994;70-74.
31. Lepesheva G.I., Podust L.M., Bellamine A., Waterman M.R. J. Biol. Chem. 276:2001;28413-28420.
32. Dawson J.A., Andersson L., Sono M. J. Biol. Chem. 257:1982;3606-3609.
33. Salerno J.C., Frey C., McMillan K., Williams R.F., Masters B.S.S., Griffith O.W. J. Biol. Chem. 270:1995;27423-27428.
34. Tsai A.L., Berka V., Chen P.F., Palmer G. J. Biol. Chem. 271:1996;32563-32571.
35. Cheesman M.R., Greenwood C., Thomson A.J. Adv. Inorg. Chem. 36:1991;201-255.
36. Shimizu T., Izuka T., Shimada H., Ishimura Y., Nozawa T., Hatano M. Biochim. Biophys. Acta. 669:1981;46-59.
37. Andersson L.A., Johnson A.K., Peterson J.A. Arch. Biochem. Biophys. 345:1997;78-87.
38. McKnight J., Cheesman M.R., Thomson A.J., Miles J.S., Munro A.W. Eur. J. Biochem. 213:1993;683-687.
39. Hildebrandt P., Stockburger M. Biochemistry. 35:1989;15029-15037.
40. Kitagawa T., Ozaki Y. Struct. Bonding. 64:1987;71-114.
41. Parthasarathi N., Hansen C., Yamaguchi S., Spiro T.G. J. Am. Chem. Soc. 109:1987;3865-3871.
42. Hagihara Y., Aimoto S., Fink A.L., Goto Y. J. Mol. Biol. 231:1993;180-184.
43. Aoyama Y., Horiuchi T., Gotoh O., Noshiro M., Yoshida Y. J. Biochem. 124 (1999) 180-184.
44. Aoyama Y., Horiuchi T., Gotoh O., Noshiro M., Yoshida Y. J. Biochem. 124:1999;694 Aoyama Y., Noshiro M., Gotoh O., Imaoka S., Funae Y., Kurosawa N., Horiuchi T., Yoshida Y. J. Biochem. 119:1996;926-933.
45. Podust L.M., Poulos T.L., Waterman M.R. Proc. Natl. Acad. Sci. USA. 98:2001;3068-3073.
46. Cupp-Vickery J.R., Garcia C., Hofacre A., McGee-Estrada K. J. Mol. Biol. 311:2001;101-110.
47. Kanemoto R.H., Powell A.T., Akiyoshi D.E., Regier D.A., Kerstetter R.A., Nester E.W., Hawes M.C., Gordon M.P. J. Bacteriol. 171:1989;2506-2512.
48. Mao Y.Q., Varoglu M., Sherman D.H. Chem. Biol. 6:1999;251-263.
49. Dairi T., Hamano Y., Igarishi Y., Furumai T., Oki T. Biosci. Biotechnol. Biochem. 61:1997;1445-1453.
50. Ost T.W.B., Miles C.S., Munro A.W., Murdoch J., Reid G.A., Chapman S.K. Biochemistry. 40:2001;13421-13429.
51. Raag R., Martinis S.A., Sligar S.G., Poulos T.L. Biochemistry. 30:1991;11420-11423.
52. Yeom H., Sligar S.G., Li H.Y., Poulos T.L., Fulco A.J. Biochemistry. 34:1995;14733-14740.