Active-site titration of serine proteases using a fluoride ion selective electrode and sulfonyl fluoride inhibitors

Analytical Biochemistry

Volumn 242, Issue 2, 1996, Pages 221-227

  Hsia, Constance Y ^a   Ganshaw, Grant ^a   Paech, Christian ^a   Murray, Christopher J ^a  

^a GENENCOR INTERNATIONAL INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; BACTERIOLOGY; ENZYMES; ION SELECTIVE ELECTRODES; IONS; SPECTROPHOTOMETERS; SPECTROPHOTOMETRY; TITRATION;

AMINO ACID ANALYSIS; BACILLUS AMYLOLIQUEFACIENS; ENZYME CONCENTRATIONS; EXTINCTION COEFFICIENTS; MOLAR EXTINCTION COEFFICIENT; PHENYLMETHYLSULFONYL FLUORIDE; PRE-STEADY STATE; SPECTROPHOTOMETRIC TITRATIONS;

FLUORINE COMPOUNDS;

AMINO ACID; BENZYLSULFONYL FLUORIDE; CHYMOTRYPSIN; FLUORIDE; FLUORIDE ION; IMIDAZOLE DERIVATIVE; PANCREATIC ELASTASE; PROTEINASE; SERINE; SERINE PROTEINASE; SUBTILISIN; THROMBIN; TRYPSIN;

ARTICLE; BACILLUS AMYLOLIQUEFACIENS; ELECTRODE; ENZYME INHIBITION; HYDROLYSIS; MEASUREMENT; NONHUMAN; PRIORITY JOURNAL; SPECTROFLUOROMETRY; SPECTROPHOTOMETRY; TITRIMETRY; TURBIDITY;

BACILLUS AMYLOLIQUEFACIENS; BACILLUS LENTUS;

EID: 0030589046     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1996.0456     Document Type: Article

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