Messenger RNA electroporation of human monocytes, followed by rapid in vitro differentiation, leads to highly stimulatory antigen-loaded mature dendritic cells

Journal of Immunology

Volumn 169, Issue 4, 2002, Pages 1669-1675

  Ponsaerts, Peter ^a   Van den Bosch, Glenn ^a   Cools, Nathalie ^a   Van Driessche, Ann ^a   Nijs, Griet ^a   Lenjou, Marc ^a   Lardon, Filip ^a   Van Broeckhoven, Christine ^a   Van Bockstaele, Dirk R ^a   Berneman, Zwi N ^a   Van Tendeloo, Viggo F I ^a  

^a UNIVERSITY OF ANTWERP   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; MESSENGER RNA; POLYCYTIDYLIC ACID;

ANTIGEN PRESENTING CELL; ARTICLE; CELL DIFFERENTIATION; CONTROLLED STUDY; DENDRITIC CELL; ELECTROPORATION; HUMAN; HUMAN CELL; IMMUNE SYSTEM; IMMUNOTHERAPY; MONOCYTE; PHENOTYPE; PRIORITY JOURNAL; T LYMPHOCYTE ACTIVATION;

EID: 0037103350     PISSN: 00221767     EISSN: None     Source Type: Journal    
DOI: 10.4049/jimmunol.169.4.1669     Document Type: Article

References (34)

1. Genebased cancer vaccines: An ex vivo approach
2. Dendritic cells and the control of immunity
3. d-binding self peptide p2Ca
4. Dendritic cells pulsed with RNA are potent antigen-presenting cells in vitro and in vivo
5. The makings of a tumor rejection antigen
6. Identification of genes coding for tumor antigens recognized by cytolytic T lymphocytes
7. Generation of dendritic cells from bone marrow progenitors using GMCSF, TNF-α, and additional cytokines: Antagonistic effects of IL-4 and IFN-γ and selective involvement of TNF-α receptor-1
8. + hematopoietic progenitor cells toward distinct dendritic cell subsets of the Birbeck granule and MIIC-positive Langerhans cell and the interdigitating dendritic cell type
9. Proliferating dendritic cell progenitors in human blood
10. Calcium ionophore-treated peripheral blood monocytes and dendritic cells rapidly display characteristics of activated dendritic cells
11. + monocyte-derived dendritic cells and implications for cancer vaccines
12. + dendritic cells is modulated under serum-free conditions by exogenously added IFN-γ and endogenously produced IL-10
13. Gene-modified dendritic cells for use in tumor vaccines
14. Vaccination with Mage-3A1 peptide-pulsed mature, monocyte-derived dendritic cells expands specific cytotoxic T cells and induces regression of some metastases in advanced stage IV melanoma
15. Induction of cellular immunity in a parathyroid carcinoma treated with tumor lysate-pulsed dendritic cells
16. Regression of human metastatic renal cell carcinoma after vaccination with tumor cell-dendritic cell hybrids
17. Nonviral transfection of distinct types of human dendritic cells: High-efficiency gene transfer by electroporation into hematopoietic progenitor- but not monocyte-derived dendritic cells
18. + dendritic cells using recombinant adenovirus suppressed T cell stimulatory capacity
19. Poxvirus as a vector to transduce human dendritic cells for immunotherapy: Abortive infection but reduced APC function
20. Highly efficient gene delivery by mRNA electroporation in human hematopoietic cells: Superiority to lipofection and passive pulsing of mRNA and to electroporation of plasmid cDNA for tumor antigen loading of dendritic cells
21. Induction of primary carcinoembryonic antigen (CEA)-specific cytotoxic T lymphocytes in vitro using human dendritic cells transfected with RNA
22. Human dendritic cells transfected with either RNA or DNA encoding influenza matrix protein MI differ in their ability to stimulate cytotoxic T lymphocytes
23. Autologous dendritic cells transfected with prostate-specific antigen RNA stimulate CTL responses against metastatic prostate tumors
24. + T cells
25. Antigen-specific inhibition of effector T cell function in humans after injection of immature dendritic cells
26. + T-cell in humans without foreign helper epitopes
27. Origin, maturation and antigen presenting function of dendritic cells
28. Polyriboinosinic polyribocytidylic acid (poly(I:C)) induces stable maturation of functionally active human dendritic cells
29. mRNA-electroporated mature dendritic cells retain transgene expression, phenotypical properties and stimulatory capacity after cryopreservation
30. Proleferation in monocyte-derived dendritic cell cultures is caused by progenitor cells capable of myeloid differentiation
31. Recognition of double-stranded RNA and activation of NF-κB by Toll-like receptor 3
32. Maturation, activation, and protection of dendritic cells induced by double-stranded RNA
33. Type I interferon as a powerful adjuvant for monocyte-derived dendritic cell development and activity in vitro and in Hu-PBL-SCID mice
34. IL-15-induced conversion of monocytes to mature dendritic cells