Quantitation of cytosine deaminase mRNA by real-time reverse transcription polymerase chain reaction: A sensitive method for assessing 5-fluorocytosine toxicity in Vitro

Analytical Biochemistry

Volumn 301, Issue 2, 2002, Pages 189-199

  Miller, C Ryan ^a,b   Gustin, Allen N ^b   Buchsbaum, Donald J ^a   Vickers, Selwyn M ^b   Manne, Upender ^c   Grizzle, William E ^c   Cloud, Gretchen A ^a   Diasio, Robert B ^a   Johnson, Martin R ^a  

^a UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

^b Kentucky   (United States)

^c University of Alabama at Birmingham   (United States)

Author keywords

5 fluorouracil; Adenovirus; Cytosine deaminase; Gene therapy

Indexed keywords

CELL CULTURE; DISEASES; ENZYME ACTIVITY; GENE THERAPY; PATIENT TREATMENT; TOXICITY; TRANSCRIPTION;

5-FLUOROURACIL; ADENOVIRUS; CYTOSINE DEAMINASE; DIFFERENTIAL SUSCEPTIBILITY; PROTEIN EXPRESSIONS; QUANTITATIVE REVERSE TRANSCRIPTION-POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION-POLYMERASE CHAIN REACTION; TRANSGENE EXPRESSION;

POLYMERASE CHAIN REACTION;

ADCMVCD FLUCYTOSINE; CYTOSINE DEAMINASE; FLUCYTOSINE; FLUOROURACIL; GENE PRODUCT; MESSENGER RNA; UNCLASSIFIED DRUG;

ADENOVIRUS; ARTICLE; CANCER SUSCEPTIBILITY; CELL HETEROGENEITY; CELLULAR DISTRIBUTION; COLON CANCER; CONTROLLED STUDY; CORRELATION ANALYSIS; DOSE RESPONSE; ENZYME ACTIVITY; ENZYME ASSAY; EXPRESSION VECTOR; GENE EXPRESSION REGULATION; GENE THERAPY; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; POPULATION RESEARCH; PREDICTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE ASSAY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TOXICITY TESTING; VIRUS REPLICATION;

ADENOVIRIDAE; VECTORS;

EID: 0037083653     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2001.5511     Document Type: Article

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