Fluorescence anisotropy studies of enzyme-substrate complex formation in stearoyl-ACP desaturase

Biochemistry

Volumn 41, Issue 49, 2002, Pages 14472-14481

  Haas, Jeffrey A ^a   Fox, Brian G ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DIMERIC RESTING;

ANISOTROPY; CATALYSIS; COMPLEXATION; DISSOCIATION; ENZYMES; RATE CONSTANTS; SUBSTRATES;

BIOCHEMISTRY;

ACYL CARRIER PROTEIN; CARBENE; CARRIER PROTEIN; DANSYL CHLORIDE; STEAROYL ACYL CARRIER PROTEIN DELTA9 DESATURASE; UNCLASSIFIED DRUG;

ACYLATION; ANISOTROPY; ARTICLE; CATALYSIS; CHEMICAL BINDING; COMPLEX FORMATION; DISSOCIATION CONSTANT; ENZYME RELEASE; ENZYME SUBSTRATE COMPLEX; EQUILIBRIUM CONSTANT; FLOW MEASUREMENT; FLUORESCENCE ANALYSIS; NONHUMAN; PRIORITY JOURNAL; STATISTICAL SIGNIFICANCE; STEADY STATE; STEREOSPECIFICITY;

ACYL CARRIER PROTEIN; ACYLATION; APOPROTEINS; BINDING SITES; DANSYL COMPOUNDS; ELECTROSTATICS; ESCHERICHIA COLI PROTEINS; FERREDOXINS; FLUORESCEIN-5-ISOTHIOCYANATE; FLUORESCENCE POLARIZATION; FLUORESCENT DYES; KINETICS; MACROMOLECULAR SUBSTANCES; MIXED FUNCTION OXYGENASES; OSMOLAR CONCENTRATION; PANTETHEINE; PROTEIN BINDING; SPECTROMETRY, FLUORESCENCE; SUBSTRATE SPECIFICITY;

INSERTION SEQUENCES;

EID: 0037058822     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi020340s     Document Type: Article

References (39)

1. Nagai, J., and Bloch, K. (1968) J. Biol. Chem. 243, 4626-4633.
2. Redenbach, M., Kieser, H. M., Denapaite, D., Eichner, A., Cullum, J., Kinashi, H., and Hopwood, D. A. (1996) Mol. Microbiol. 21, 77-96.
3. Cole, S. T., Brosch, R., Parkhill, J., Garnier, T., Churcher, C., Harris, D., Gordon, S. V., Eiglmeier, K., Gas, S., Barry, C. E., III, Tekaia, F., Badcock, K., Basham, D., Brown, D., Chillingworth, T., Connor, R., Davies, R., Devlin, K., Feltwell, T., Gentles, S., Hamlin, N., Holroyd, S., Hornsby, T., Jagels, K., Barrell, B. G., et al. (1998) Nature 393, 537-544.
4. Fox, B. G., Shanklin, J., Somerville, C., and Münck, E. (1993) Proc. Natl. Acad. Sci. U.S.A. 90, 2486-2490.
5. van de Loo, F. J., Fox, B. G., and Somerville, C. (1993) in Lipid Metabolism in Plants (Moore, T. E., Ed.) pp 91-126, CRC Press, Boca Raton, FL.
6. Fox, B. G., Shanklin, J., Ai, J., Loehr, T. M., and Sanders-Loehr, J. (1994) Biochemistry 33, 12776-12786.
7. Fox, B. G. (1998) in Comprehensive Biological Catalysis (Sinnott, M., Ed.) pp 261-348, Academic Press, London.
8. Ryle, M. J., and Hausinger, R. P. (2002) Curr. Opin. Chem. Biol. 6, 193-201.
9. Nordlund, P., and Eklund, H. (1995) Curr. Opin. Struct. Biol. 5, 758-766.
10. Merkx, M., Kopp, D. A., Sazinsky, M. H., Blazyk, J. L., Muller, J., and Lippard, S. J. (2001) Angew. Chem., Int. Ed. Engl. 40, 2782-2807.
11. Solomon, E. I., Brunold, T. C., Davis, M. I., Kemsley, J. N., Lee, S.-K., Lehnert, N., Neese, F., Skulan, A. J., Yang, Y.-S., and Zhou, J. (2000) Chem. Rev. 100, 235-349.
12. Lindqvist, Y., Huang, W., Schneider, G., and Shanklin, J. (1996) EMBO J. 15, 4081-4092.
13. Shu, L., Broadwater, J. A., Achim, C., Fox, B. G., Münck, E., and Que, L., Jr. (1998) J. Biol. Inorg. Chem. 3. 392-400.
14. Broadwater, J. A., Ai, J., Loehr, T. M., Sanders-Loehr, J., and Fox, B. G. (1998) Biochemistry 37, 14664-14671.
15. Yang, Y.-S., Broadwater, J. A., Fox, B. G., and Solomon, E. I. (1999) J. Am. Chem. Soc. 121, 2770-2783.
16. Broadwater, J. A., Achim, C., Münck, E., and Fox, B. G. (1999) Biochemistry 38, 12197-12204.
17. Haas, J. A., and Fox, B. G. (1999) Biochemistry 38, 12833-12840.
18. Lyle, K. S., Ai, J., Moenne-Loccoz, P., Sanders-Loehr, J., Loehr, T. M., and Fox, B. G. (2000) Biochemistry 39, 10507-10513.
19. Whittle, E., and Shanklin, J. (2001) J. Biol. Chem. 276, 21500-21505.
20. Haas, J. A., Frederick, M. A., and Fox, B. G. (2000) Protein Expression Purif. 20, 274-284.
21. Hoffman, B. J., Broadwater, J. A., Johnson, P., Harper, J., Fox, B. G., and Kenealy, W. R. (1995) Protein Expression Purif, 6, 646-654.
22. Haugland, R. P. (1996) in Handbook of Fluorescent Probes and Research Chemicals, 6th Ed., p 679, Molecular Probes, Eugene, OR.
23. Lakowicz, J. R. (1999) in Principles of Fluorescence Spectroscopy, 2nd Ed., Kluwer Academic/Plenum Publishers, New York.
24. Mendel, C. M., and Mendel, D. B. (1985) Biochem. J. 228, 269-272.
25. Abita, J. P., Lzdunski, M., and Ailhaud, G. P. (1971) Eur. J. Biochem. 23, 412-420.
26. Prescott, D. J., and Vagelos, P. R. (1972) Adv. Enzymol. 36, 269-311.
27. Broadwater, J. A., and Fox, B. G. (1998) Protein Expression Purif. 15, 314-326.
28. Holak, T. A., Kearsley, S. K., Kim, Y., and Prestegard, J. H. (1988) Biochemistry 27, 6135-6142.
29. Danliker, W. B., Hsu, M.-L., Levin, J., and Rao, B. R. (1981) Methods Enzymol. 74, 3-28.
30. Lunblad, J. R., Laurance, M., and Goodman, R. H. (1996) Mol. Endocrinol. 10, 607-612.
31. Gibson, K. J. (1993) Biochim. Biophys. Acta 1169, 231-235.
32. McKeon, T. A., and Stumpf, P. K. (1982) J. Biol. Chem. 257, 12141-12147.
33. Heyduk, T., Ma, Y., Tang, H., and Ebright, R. H. (1996) Methods Enzymol. 274, 492-505.
34. Hill, J. J., and Royer, C. A. (1997) Methods Enzymol. 278, 390-416.
35. Heath, R. J., White, S. W., and Rock, C. O. (2001) Prog. Lipid Res. 40, 467-497.
36. Segel, I. H. (1975) in Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems, p 957, John Wiley and Sons, New York.
37. Kim, Y., and Prestegard, J. (1990) Proteins: Struct., Funct., Genet. 8, 377-385.
38. Northrop, D. B. (1977) in Isotope Effects on Enzyme-Catalyzed Reactions (Cleland, W. W., O'Leary, M., and Northrup, D. B., Eds.) pp 122-157, University Park Press, Baltimore, MD.
39. Northrop, D. B. (1999) in Enzymatic Mechanisms (Frey, P. A., and Northrop, D., Eds.) pp 250-263, IOS Press, Amsterdam.