Circular dichroism and magnetic circular dichroism studies of the reduced binuclear non-heme iron site of stearoyl-ACP Δ9-desaturase: Substrate binding and comparison to ribonucleotide reductase

Journal of the American Chemical Society

Volumn 121, Issue 12, 1999, Pages 2770-2783

  Yang, Yi Shan ^a   Broadwater, John A ^b   Pulver, Sabine Coates ^a   Fox, Brian G ^b   Solomon, Edward I ^a  

^a STANFORD UNIVERSITY   (United States)

^b UNIVERSITY OF WISCONSIN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME; OXYGEN; RIBONUCLEOTIDE REDUCTASE; STEAROYL ACYL CARRIER PROTEIN DESATURASE; UNCLASSIFIED DRUG;

ARTICLE; CHEMICAL REACTION; CIRCULAR DICHROISM; ENZYME ACTIVE SITE; ENZYME ANALYSIS; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; FATTY ACID DESATURATION; STRUCTURE ANALYSIS; X RAY ANALYSIS;

EID: 0033620444     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja9822714     Document Type: Article

References (73)

1. Fox, B. G. Catalysis by Non-Heme Iron-Containing Enzymes. In Comprehensive Biological Catalysis; Sinnott, M., Ed.; Academic Press: London, 1997; Vol. 3, pp 261-348.
2. Wallar, B.; Lipscomb, J. D. Chem. Rev. 1996, 96, 2625-2657.
3. Feig, A. L.; Lippard, S. J. Chem. Rev. 1994, 94, 759-805.
4. Stenkamp, R. E. Chem. Rev. 1994, 94, 715-726.
5. Holmes, M. A.; Trong, I. L.; Turley, S., Sieker, L. C.; Stenkamp, R. E. J. Biol. Chem. 1991, 218, 583-593.
6. Holmes, M. A.; Stenkamp, R. E. J. Biol. Chem. 1991, 220, 723-737.
7. Rosenzweig, A. C.; Frederick, C. A.; Lippard, S. J.; Nordlund, P. Nature 1993, 366, 537-543.
8. Rosenzweig, A. C.; Nordlund, P.; Takahara, P. M.; Frederick, C. A.; Lippard, S. J. Chem. Biol. 1995, 2, 409-418.
9. Pikus, J. D.; Studts, J. M.; Achim, C.; Kauffmann, K. E.; Münck, E.; Steffan, R. J.; McClay, K.; Fox, B. G. Biochemistry 1996, 35, 9106-9119.
10. Shanklin, J.; Achim, C.; Schmidt, H.; Fox, B. G.; Münck, E. Proc. Natl. Acad. Sci. U.S.A. 1997, 94, 2981-2986.
11. Ravi, N.; Bollinger, J. M. Jr.; Huynh, B. H.; Edmondson, D. E.; Stubbe, J. J. Am. Chem. Soc. 1994, 116, 8007-8014.
12. Bollinger, J. M. Jr.; Tong, W. H.; Ravi, N.; Huynh, B. H.; Edmondson, D. E.; Stubbe, J. J. Am. Chem. Soc. 1994, 116, 8015-8023.
13. Bollinger, J. M. Jr.; Tong, W. H.; Ravi, N.; Huynh, B. H.; Edmondson, D. E.; Stubbe, J. J. Am. Chem. Soc. 1994, 116, 8024-8032.
14. Nordlund, P.; Eklund, H. J. Biol. Chem. 1993, 232, 123-164.
15. Logan, D. T.; Su, X.-D.; Åberg, A.; Regnström, K.; Hajdu, J.; Eklund, H.; Nordlund, P. Structure 1996, 4, 1053-1064.
16. Shanklin, J.; Cahoon, E. B. Annu. Rev. Plant Physiol. Plant Mol. Biol. 1998, 49, 611-641.
17. Lindqvist, Y.; Huang, W.; Schneider, G.; Shanklin, J. EMBO J. 1996, 15, 4081-4092.
18. Klabunde, T.; Krebs, B. Struct. Bonding 1997, 89, 177-198.
19. Klabunde, T.; Sträter, N.; Fröhlich, R.; Witzel, H.; Krebs, B. J. Biol. Chem. 1996, 259, 737-748.
20. Bonomi, F.; Kurtz, D. M. Jr.; Cui, X. J. Biol. Inorg. Chem. 1996, 1, 67-72.
21. deMaré, F.; Kurtz, D. M. Jr.; Nordlund, P. Nat. Struct. Biol. 1996, 3, 539-546.
22. Pierik, A. J.; Wolbert, R. B. G.; Portier, G. L.; Verhagen, M. F. J. M.; Hagen, W. R. Eur. J. Biochem. 1993, 212, 237-245.
23. Kurtz, D. M. Jr. J. Biol. Inorg. Chem. 1997, 2, 159-167.
24. Que, L. Jr.; Dong, T.; Shu, L.; Wilkinson, E. C. In Spectroscopic Methods in Bioinorganic Chemistry; Solomon, E. I., Hodgson, K. O., Eds.; ACS Symposium Series; American Chemical Society: Washington, DC, 1998; Vol. 692, pp 374-386.
25. Bollinger, J. M., Jr.; Krebs, C.; Vicol, A.; Chen, S.; Ley, B. A.; Edmondson, D. E.; Huynh, B. H. J. Am. Chem. Soc. 1998, 120, 1094-1095.
26. Bollinger, J. M. Jr.; Edmondson, D. E.; Huynh, B. H.; Filley, J.; Norton, J. R.; Stubbe, J. Science 1991, 253, 292-298.
27. Gunstone, F. D. In Fatty Acid and Lipid Chemistry, 1st ed.; Blackie Academic & Professional: London, 1996.
28. Fatty Acid Metabolism and Its Regulation; Numa, S., Ed.; Elsevier Science Publishers B. V.; New York, 1984.
29. Nagai, J.; Bloch, K. J. Biol. Chem. 1966, 241, 1925-1927.
30. Holloway, P. W. In The Enzymes, 3rd ed.; Boyer, P. D., Ed.; Academic: New York, 1983; pp 63-83.
31. Nagai, J.; Bloch, K. J. Biol. Chem. 1996, 243, 4626-4633.
32. Schmidt, H.; Heinz, E. Plant Physiol. 1990, 94, 214-220.
33. Wada, H.; Schmidt, H.; Heinz, E.; Murata, N. J. Bacteriol. 1993, 175, 544-547.
34. Shanklin, J.; Somerville, C. Proc. Natl. Acad. Sci. U.S.A. 1991, 88, 2510-2514.
35. Fox, B. G.; Shanklin, J.; Ai, J.; Loehr, T. M.; Sanders-Loehr, J. Biochemistry 1994, 33, 12776-12786.
36. Schiemke, A. K.; Loehr, T. M.; Sanders-Loehr, J. J. Am. Chem. Soc. 1986, 108, 2437-2443.
37. Gay, R. R.; Solomon, E. I. J. Am. Chem. Soc. 1978, 100, 1972.
38. Sjöberg, B.-M.; Loehr, T. M.; Sanders-Loehr, J. Biochemistry 1982, 21, 96-102.
39. Ling, J.; Sahlin, M.; Sjöberg, B. M.; Loehr, T. M.; Sanders-Loehr, J. J. Biol. Chem. 1994, 269, 5595-5601.
40. Dave, B. C.; Czernuszewicz, R. S.; Prickril, B. C.; Kurtz, D. M., Jr. Biochemistry 1994, 33, 3572-3576.
41. Fox, B. G.; Shanklin, J.; Somerville, C.; Münck, E. Proc. Natl. Acad. Sci. U.S.A. 1993, 90, 2486-2490.
42. Tong, W. H.; Chen, S.; Lloyd, S. G.; Edmondson, D. E.; Huynh, B.-H.; Stubbe, J. J. Am. Chem. Soc. 1996, 118, 2107-2108.
43. Broadwater, J. A.; Ai, J.; Loehr, T. M.; Sanders-Loehr, J. Fox, B. G. Biochemistry 1998, 37, 14664-14671.
44. Cahoon, E. B.; Lindqvist, Y.; Schneider, G.; Shanklin, J. Proc. Natl. Acad. Sci. U.S.A. 1997, 94, 4872-4877.
45. Pulver, S.; Froland, W. A.; Fox, B. G.; Lipscomb, J. D., Solomon, E. I. J. Am. Chem. Soc. 1993, 115, 12409-12422.
46. Reem, R. C.; Solomon, E. I. J. Am. Chem. Soc. 1987, 109, 1216-1226.
47. Hoffman, B. J.; Broadwater, J. A.; Johnson, P. A.; Harper, J.; Fox, B. G.; Kenealy, W. R. Protein Express. Purif. 1995, 6, 646-654.
48. Lambolot, R. H.; Walsh, C. T. J. Biol. Chem. 1995, 270, 24658-24661.
49. Rock, C. O.; Garwin, J. L. J. Biol. Chem. 1979, 254, 7123-7128.
50. Broadwater, J. A.; Fox, B. G. Manuscript in preparation.
51. Pavel, E. G.; Kitajima, N.; Solomon, E. I. J. Am. Chem. Soc. 1998, 120, 3949-3962.
52. Companion, A. L.; Komarynsky, M. A. J. Chem. Educ. 1964, 41, 257-262.
53. Long, G. L.; Clarke, P. J. Inorg. Chem. 1978, 17, 1394-1401.
54. Miller, L. L.; Jacobson, R. A.; Chen, Y.-S.; Kurtz, D. M. Acta Crystallogr. 1989, C45, 527-529.
55. Vaira, D. M.; Orioli, P. L. Acta Crystallogr. 1968, B24, 1269-1272.
56. Montgomery, H.; Chastain, R. V.; Natt, J. J.; Witkowska, A. M.; Lingafelter, E. C. Acta Crystallogr. 1967, 22, 775-780.
57. Rose, N. J.; Drago, R. S. J. Am. Chem. Soc. 1959, 81, 6138-6141.
58. 2g Hamiltonian rather than a spin Hamiltonian since there is orbital as well as spin angular momentum. However, near the rhombic limit appropriate for these sites (vide infra) these give similar results. See ref 66.
59. Kahn, O. Molecular Magnetism; VCH Publishers: New York, 1993; Chapter 7.
60. Weltner, W., Jr. Magnetic Atoms and Molecules; Dover Publications: New York, 1983; pp 304-339
61. Bencini, A.; Gatteschi, D. Electron Paramagnetic Resonance of Exchanged Coupled System; Springer-Verlag: Berlin-Heidelberg, 1990; Chapter 2.
62. Moriya, T. In Magnetism; Rado, G. T., Suhl, H., Eds.; Academic Press Inc.: New York and London, 1963; Vol. 1, pp 85-124.
63. Moriya, T. Phys. Rev. 1960, 120, 91-98.
64. Browett, W. R.; Fucaloro, A. F.; Morgan, T. V.; Stephens, P. J. J. Am. Chem. Soc. 1983, 105, 1868-1872.
65. Bennett, D. E.; Johnson, M. K. Biochim. Biophys. Acta 1987, 911, 71-80.
66. Solomon, E. I.; Pavel, E. G.; Loeb, K. E.; Campochiaro, C. Coord. Chem. Rev. 1995, 144, 369-460.
67. Neese, F.; Solomon, E. I. Inorg. Chem., submitted for publication.
68. -1 were initially fit independently. g∥ = ∼4 for the ground sublevel and g∥ = ∼8 for the first excited sublevel are required for both data sets, which indicates that these two bands must reflect the same ground and excited sublevels (vide infra).
69. Ménage, S.; Zang, Y.; Hendrich, M. P.; Que, L., Jr. J. Am. Chem. Soc. 1992, 114, 7786-7792.
70. Pulver, S. C.; Tong, W. H.; Bollinger, M. J., Jr.; Stubbe, J.; Solomon, E. I. J. Am. Chem. Soc. 1995, 117, 12664-12678.
71. Pulver, S. C.; Froland, W. A.; Lipscomb, J. D.; Solomon, E. I. J. Am. Chem. Soc. 1997, 119, 387-395.
72. Himmelwright, R. S.; Eickman, N. C.; Solomon, E. I. J. Am. Chem. Soc. 1979, 101, 1576-1586.
73. Westmoreland, T. D.; Wilcox, D. E.; Baldwin, M. J.; Mims, W. B.; Solomon, E. I. J. Am. Chem. Soc. 1989, 111, 6106-6123.