Three-dimensional fibroblast-tumor cell interaction causes downregulation of rack1 mRNA expression in breast cancer cells in vitro

International Journal of Cancer

Volumn 102, Issue 2, 2002, Pages 129-136

  Seidl, Paula ^a   Huettinger, Robert ^a   Knuechel, Ruth ^a   Kunz Schughart, Leoni A ^a  

^a UNIVERSITY OF REGENSBURG   (Germany)

Author keywords

Breast tumor cell; Coculture; Fibroblast; rack1; RNA arbitrarily primed PCR; Spheroid

Indexed keywords

COMPLEMENTARY DNA; GUANINE NUCLEOTIDE BINDING PROTEIN; MESSENGER RNA; PROTEIN RACK1; UNCLASSIFIED DRUG;

ARTICLE; BREAST CANCER; CANCER CELL CULTURE; CELL INTERACTION; CELL STRAIN MCF 7; FIBROBLAST; FLUORESCENCE ACTIVATED CELL SORTING; GENE EXPRESSION REGULATION; HUMAN; HUMAN CELL; NORTHERN BLOTTING; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SINGLE STRAND CONFORMATION POLYMORPHISM; SPHEROID CELL;

BASE SEQUENCE; BREAST NEOPLASMS; CELL COMMUNICATION; COCULTURE TECHNIQUES; DOWN-REGULATION; FEMALE; FIBROBLASTS; GENE EXPRESSION REGULATION, NEOPLASTIC; GTP-BINDING PROTEINS; HUMANS; MOLECULAR SEQUENCE DATA; NEOPLASM PROTEINS; PROTEIN KINASE C; RECEPTORS, CELL SURFACE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SPHEROIDS, CELLULAR; SRC HOMOLOGY DOMAINS; TUMOR CELLS, CULTURED;

EID: 0037058309     PISSN: 00207136     EISSN: None     Source Type: Journal    
DOI: 10.1002/ijc.10675     Document Type: Article

References (38)

1. Kunz-Schughart LA, Knuechel R. Tumor-associated fibroblasts (part I): active stromal participants in tumor development and progression? Histol Histopathol 2002;17:599-621.
2. Kunz-Schughart LA, Knuechel R. Tumor-associated-fibroblasts (part II): functional impact on tumor tissue. Histol Histopathol 2002;17:623-37.
3. Wernert N. The multiple roles of tumour stroma. Virchows Arch 1997;430:433-43.
4. Hayward SW, Grossfeld GD, Tlsty TD, Cunha GR. Genetic and epigenetic influences in prostatic carcinogenesis. Int J Oncol 1998;13:35-47.
5. Elenbaas B, Weinberg RA. Heterotypic signaling between epithelial tumor cells and fibroblasts in carcinoma formation. Exp Cell Res 2001;264:169-84.
6. Tlsty TD, Hein PW. Know thy neighbor: stromal cells can contribute oncogenic signals. Curr Opin Genet Dev 2001;11:54-9.
7. Kunz-Schughart LA, Heyder P, Schroeder J, Knuechel R. A heterologous 3-D coculture model of breast tumor cells and fibroblasts to study tumor-associated fibroblast differentiation. Exp Cell Res 2001;266:74-86.
8. Schuster U, Büttner R, Hofstädter F, Knüchel R. A heterologous in vitro coculture system to study interaction between human bladder cancer cells and fibroblasts. J Urol 1994;151:1707-11.
9. Welsh J, McClelland M. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 1990;18:7213-8.
10. McClelland M, Welsh J. RNA fingerprinting by arbitrarily primed PCR. PCR Methods Appl 1994;4:S66-81.
11. McClelland M, Arensdorf H, Cheng R, Welsh J. Arbitrarily primed PCR fingerprints resolved on SSCP gels. Nucleic Acids Res 1994;22:1770-1.
12. Seidl J, Knuechel R, Kunz-Schughart LA. Evaluation of membrane physiology following fluorescence activated or magnetic cell separation. Cytometry 1999;36:102-11.
13. Schlegel J, Vogt T, Munkel K, Rüschoff J. DNA fingerprinting of mammalian cell lines using nonradioactive arbitrarily primed PCR (AP-PCR). Biotechniques 1996;20:178-80.
14. Ron D, Chen CH, Caldwell J, Jamieson L, Orr E, Mochly-Rosen D. Cloning of an intracellular receptor for protein kinase C: a homolog of the beta subunit of G proteins. Proc Natl Acad Sci USA 1994;91:839-43.
15. Guillemot F, Billault A, Auffray C. Physical linkage of a guanine nucleotide-binding protein-related gene to the chicken major histocompatibility complex. Proc Natl Acad Sci USA 1989;86:4594-8.
16. Liang P, Pardee AB. Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science 1992;257:967-71.
17. Welsh J, Chada K, Dalal SS, Cheng R, Ralph D, McClelland M. Arbitrarily primed PCR fingerprinting of RNA. Nucleic Acids Res 1992;20:4965-70.
18. Milner JJ, Cecchini E, Dominy PJ. A kinetic model for subtractive hybridization. Nucleic Acids Res 1995;23:176-87.
19. Hubank M, Schatz DG. Identifying differences in mRNA expression by representational difference analysis of cDNA. Nucleic Acids Res 1994;22:5640-8.
20. Lavery DJ, Fonjallaz P, Fleury-Olela F, Schibler U. Analysis of differential gene expression using the SABRE enrichment protocol. Methods Mol Biol 2000;99:321-45.
21. Khan J, Bittner ML, Chen Y, Meltzer PS, Trent JM. DNA microarray technology: the anticipated impact on the study of human disease. Biochim Biophys Acta 1999;1423:M17-28.
22. Kawasaki ES, Chen A. Analysis of gene sequences by hybridization of PCR-amplified DNA to covalently bond oligonucleotide probes: the reverse dot blot method. In: Isaacs WB, ed. Protocols for nucleic acid analysis by nonradioactive probes. Totowa, NJ: Humana Press, 1994, 225-36.
23. Wang T, Brown MJ. mRNA quantification by real time TaqMan polymerase chain reaction: validation and comparison with RNase protection. Anal Biochem 1999;269:198-201.
24. Schor SL. Fibroblast subpopulations as accelerators of tumor progression: the role of migration stimulating factor. EXS 1995;74:273-96.
25. Powell DW, Mifflin RC, Valentich JD, Crowe SE, Saada JI, West AB. Myofibroblasts. I. Paracrine cells important in health and disease. Am J Physiol 1999;277:C1-9.
26. Kurose K, Hoshaw-Woodard S, Adeyinka A, Lemeshow S, Watson H, Eng C. Genetic model of multi-step breast carcinogenesis involving the epithelium and stroma: clues to tumour-microenvironment interactions. Hum Mol Genet 2001;10:1907-13.
27. Moinfar F, Man YG, Arnould L, Bratthauer GL, Ratschek M, Tavassoli FA. Concurrent and independent genetic alterations in the stromal and epithelial cells of mammary carcinoma: implications for tumorigenesis. Cancer Res 2000;60:2562-6.
28. Wernert N, Hugel A, Locherbach C. Genetic alterations in the fibroblastic stroma of invasive colon and breast carcinomas [in German]. Verh Dtsch Ges Pathol 1998;82:317-21.
29. Ron D, Kazanietz MG. New insights into the regulation of protein kinase C and novel phorbol ester receptors. FASEB J 1999;13:1658-76.
30. Disatnik MH, Hernandez-Sotomayor SM, Jones G, Carpenter G, Mochly-Rosen D. Phospholipase C-gammal binding to intracellular receptors for activated protein kinase C. Proc Natl Acad Sci USA 1994;91:559-63.
31. Chang BY, Conroy KB, Machleder EM, Cartwright CA. RACK1, a receptor for activated C kinase and a homolog of the beta subunit of G proteins, inhibits activity of src tyrosine kinases and growth of NIH 3T3 cells. Mol Cell Biol 1998;18:3245-56.
32. Rodriguez MM, Ron D, Touhara K, Chen CH, Mochly-Rosen D. RACK1, a protein kinase C anchoring protein, coordinates the binding of activated protein kinase C and select pleckstrin homology domains in vitro. Biochemistry 1999;38:13787-94.
33. Liliental J, Chang DD. Rack1, a receptor for activated protein kinase C, interacts with integrin beta subunit. J Biol Chem 1998;273:2379-83.
34. Yarwood SJ, Steele MR, Scotland G, Houslay MD, Bolger GB. The RACK1 signaling scaffold protein selectively interacts with the cAMP-specific phosphodiesterase PDE4D5 isoform. J Biol Chem 1999;274:14909-17.
35. Mochly-Rosen D, Miller KG, Scheller RH, Khaner H, Lopez J, Smith BL. p65 fragments, homologous to the C2 region of protein kinase C. bind to the intracellular receptors for protein kinase C. Biochemistry 1992;31:8120-4.
36. Usacheva A, Smith R, Minshall R, Baida G, Seng S, Croze E, Colamonici O. The WD motif-containing protein receptor for activated protein kinase C (RACK1) is required for recruitment and activation of signal transducer and activator of transcription 1 through the type I interferon receptor. J Biol Chem 2001;276:22948-53.
37. Koehler JA, Moran MF. RACK1, a protein kinase C scaffolding protein, interacts with the PH domain of p120GAP. Biochem Biophys Res Commun 2001;283:888-95.
38. Berns H, Humar R, Hengerer B, Kiefer FN, Battegay EJ. RACK1 is up-regulated in angiogenesis and human carcinomas. FASEB J 2000;14:2549-58.