Dual-wavelength fluorescent speckle microscopy reveals coupling of microtubule and actin movements in migrating cells

Journal of Cell Biology

Volumn 158, Issue 1, 2002, Pages 31-37

  Salmon, Wendy C ^a   Adams, Michael C ^a   Waterman Storer, Clare M ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

Cell motility; Cytoskeleton; Dynamic instability; Live cell microscopy; Retrograde flow

Indexed keywords

F ACTIN;

ACTIN FILAMENT; ANIMAL CELL; ARTICLE; CELL INTERACTION; CELL MIGRATION; CONFOCAL MICROSCOPY; CONTROLLED STUDY; DYNAMICS; FLOW RATE; LAMELLIPODIUM; LUNG ALVEOLUS EPITHELIUM; MICROTUBULE; NONHUMAN; PRIORITY JOURNAL; VELOCITY;

ACTINS; ANIMALS; CELL MOVEMENT; EPITHELIAL CELLS; IMAGE PROCESSING, COMPUTER-ASSISTED; LUNG; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; MICROSCOPY, VIDEO; MICROTUBULES; SALAMANDRIDAE; TIME FACTORS;

ANIMALIA; SALAMANDRIDAE;

EID: 0037043342     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.200203022     Document Type: Article

References (24)

1. Role of actin-filament disassembly in lamellipodium protrusion in motile cells revealed using the drug jasplakinolide
2. Identification of novel graded polarity actin filament bundles in locomoting heart fibroblasts: Implications for the generation of motile force
3. Axon branching requires interactions between dynamic microtubules and actin filaments
4. Integrin-mediated activation of Cdc42 controls cell polarity in migrating astrocytes through PKCξ
5. Bridging cytoskeletal intersections
6. A micromachined device provides a new bend on fibroblast traction forces
7. Protein kinase C activation promotes microtubule advance in neuronal growth cones by increasing average microtubule growth lifetimes
8. Microtubule targeting of substrate contacts promotes their relaxation and dissociation
9. Drosophila APC2 and armadillo participate in tethering mitotic spindles to cortical actin
10. Actin-based cell motility and cell locomotion
11. Cdc42, dynein and dynactin regulate MTOC reorientation independent of Rho-regulated microtubule stabilization
12. High resolution detection of mechanical forces exerted by locomoting fibroblasts on the substrate
13. Control of spindle polarity and orientation in Saccharomyces cerevisiae
14. Filopodia and actin arcs guide the assembly and transport of two populations of microtubules with unique dynamic parameters in neuronal growth cones
15. Assembling an actin cytoskeleton for cell attachment and movement
16. Single-molecule speckle analysis of actin filament turnover in lamellipodia
17. Fluorescent speckle microscopy (FSM) of microtubules and actin in living cells
18. Actomyosin-based retrograde flow of microtubules in the lamella of migrating epithelial cells influences microtubule dynamic instability and turnover and is associated with microtubule breakage and treadmilling
19. Fluorescent speckle microscopy, a method to visualize the dynamics of protein assemblies in living cells
20. Microtubules remodel actomyosin networks in Xenopus egg extracts via two mechanisms of F-actin transport
21. Cell motility: Can Rho GTPases and microtubules point the way?
22. Region-specific microtubule transport in motile cells
23. Antagonistic forces generated by myosin II and cytoplasmic dynein regulate microtubule turnover, movement, and organization in interphase cells
24. Focal loss of actin bundles causes microtubule redistribution and growth cone turning