Rapid exchange of mammalian topoisomerase IIα at kinetochores and chromosome arms in mitosis

Journal of Cell Biology

Volumn 158, Issue 1, 2002, Pages 23-29

  Tavormina, Penny A ^a,b   Côme, Marie George ^a   Hudson, Joanna R ^a   Mo, Yin Yuan ^c   Beck, William T ^c   Gorbsky, Gary J ^a  

^a UNIVERSITY OF OKLAHOMA COLLEGE OF MEDICINE   (United States)

^b Universal Imaging Corporation   (United States)

^c UNIVERSITY OF ILLINOIS AT CHICAGO   (United States)

Author keywords

Cell cycle proteins; Cell division; DNA topoisomerase II; Kinetochore; Nucleolus

Indexed keywords

DNA TOPOISOMERASE (ATP HYDROLYSING);

ANAPHASE; ARTICLE; BLEACHING; CATALYSIS; CELL LINE; CELL ORGANELLE; CENTROMERE; CHROMOSOME ARM; CHROMOSOME CONDENSATION; CHROMOSOME SEGREGATION; COMPLEX FORMATION; CONTROLLED STUDY; INTERPHASE; MAMMAL; MITOSIS; NONHUMAN; NUCLEOLUS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; PROTEIN TRANSPORT;

ANIMALS; ANTIGENS, NEOPLASM; BLOTTING, WESTERN; CELL LINE; CHROMOSOMES; CYTOPLASM; DNA TOPOISOMERASES, TYPE II, EUKARYOTIC; DNA-BINDING PROTEINS; DOWN-REGULATION; GREEN FLUORESCENT PROTEINS; KINETOCHORES; LASERS; LUMINESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; MITOSIS; PRECIPITIN TESTS; RECOMBINANT FUSION PROTEINS; SUBCELLULAR FRACTIONS; SWINE;

MAMMALIA; STAPHYLOCOCCUS PHAGE 187;

EID: 0037043338     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.200202053     Document Type: Article

References (28)

1. Functional and regulatory characteristics of eukaryotic type II DNA topoisomerase
2. Active DNA topoisomerase IIα is a component of the salt-stable centrosome core
3. Dynamics of human DNA topoisomerases IIα and IIβ in living cells
4. The dynamics of postmitotic reassembly of the nucleolus
5. Topoisomerase II is a structural component of mitotic chromosome scaffolds
6. Localization of topoisomerase II in mitotic chromosomes
7. Cell cycle progression and chromosome segregation in mammalian cells cultured in the presence of the topoisomerase II inhibitors ICRF-187 ([+]-1,2-bis[3,5-dioxopiperazinyl-1-yl]propane; ADR-529) and ICRF-159 (razoxane)
8. Microtubules in the metaphase-arrested mouse oocyte turn over rapidly
9. Differential expression of DNA topoisomerases I and II during the eukaryotic cell cycle
10. Topoisomerase II does not play a scaffolding role in the organization of mitotic chromosomes assembled in Xenopus egg extracts
11. Visualization of Mad2 dynamics at kinetochores, along spindle fibers, and at spindle poles in living cells
12. DNA Topoisomerase II must act at mitosis to prevent nondisjunction and chromosome breakage
13. Mammalian p55CDC mediates association of the spindle checkpoint protein Mad2 with the cyclosome/anaphase-promoting complex, and is involved in regulating anaphase onset and late mitotic events
14. Merbarone, a catalytic inhibitor of DNA topoisomerase II, induces apoptosis in CEM cells through activation of ICE/CED-3-like protease
15. Cell cycle-coupled relocation of types I and II topoisomerases and modulation of catalytic enzyme activities
16. Association of human DNA topoisomerase IIα with mitotic chromosomes in mammalian cells is independent of its catalytic activity
17. Overexpression of human DNA topoisomerase II alpha by fusion to enhanced green fluorescent protein
18. The nucleolus: An old factory with unexpected capabilities
19. Two differentially spliced forms of topoisomerase IIα and β mRNAs are conserved between birds and humans
20. High mobility of proteins in the mammalian cell nucleus
21. Topoisomerase IIα is associated with the mammalian centromere in a cell cycle- and species-specific manner and is required for proper centromere/kinetochore structure
22. Antitumor bisdioxopiperazines inhibit yeast DNA topoisomerase II by trapping the enzyme in the form of a closed protein clamp
23. The distribution of topoisomerase II on mammalian chromosomes
24. Multiple chromosomal populations of topoisomerase II detected in vivo by time-lapse, three dimensional wide-field microscopy
25. DNA topoisomerase IIα is the major chromosome protein recognized by the mitotic phosphoprotein antibody MPM-2
26. DNA topoisomerase II is required for condensation and separation of mitotic chromosomes in S. pombe
27. ATP-bound topoisomerase II as a target for antitumor drugs
28. Untangling the role of DNA topoisomerase II in mitotic chromosome structure and function