Overexpression of human DNA topoisomerase IIα by fusion to enhanced green fluorescent protein

BioTechniques

Volumn 25, Issue 6, 1998, Pages 1052-1057

  Mo, Yin Yuan ^a   Ameiss, Keith A ^a   Beck, William T ^a  

^a UNIVERSITY OF ILLINOIS AT CHICAGO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; DNA TOPOISOMERASE (ATP HYDROLYSING); GREEN FLUORESCENT PROTEIN; TENIPOSIDE;

ARTICLE; CANCER RESISTANCE; CONTROLLED STUDY; DRUG TARGETING; ENZYME LOCALIZATION; ENZYME MECHANISM; GENE FUSION; GENE OVEREXPRESSION; GENE TARGETING; GENETIC TRANSFECTION; HELA CELL; HUMAN; HUMAN CELL;

CYTOMEGALOVIRUS;

EID: 0032414744     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98256cr04     Document Type: Article

References (10)

1. Asano, T., T. An, J. Mayes, L.A. Zwelling and E.S. Kleinerman. 1996. Transfection of human topoisomerase IIα into etoposide-resistant cells: transient increase in sensitivity followed by down-regulation of the endogenous gene. Biochem. J. 319:307-313.
2. Boege, F., A. Andersen, S. Jensen, R. Zeidler and H. Kreipe. 1995. Proliferation-associated nuclear antigen Ki-S1 is identical with topoisomerase IIα: delination of a carboxyl-terminal epitope with peptide antibodies. Am. J. Pathol. 146:1302-1308.
3. Goswami, P.C., J.L. Roti and C.R. Hunt. 1996. The cell cycle-coupled expression of topoisomerase II alpha during S phase is regulated by mRNA stability and is disrupted by heat shock or ionizing radiation. Mol. Cell. Biol. 16:1500-1508.
4. Hsiung, Y., M. Jannatipour, A. Rose, J. McMahon, D. Duncan and J.L. Nitiss. 1996. Functional expression of human topoisomerase II alpha in yeast: mutations at amino acids 450 or 803 of topoisomerase II alpha result in enzymes that can confer resistance to anti-topoisomerase II agents. Cancer Res. 56:91-99.
5. Jenkins, J.R., P. Ayton, T. Jones, S.L. Davies, D.L. Simmons, A.L. Harris, D. Sheer and I.D. Hickson. 1992. Isolation of cDNA clones encoding the beta isozyme of human DNA topoisomerase II and localization of the gene to chromosome 3p24. Nucleic Acids Res. 20:5587-5592.
6. Kozak, M. 1987. At least six nucleotides preceding the AUG initiator codon enhance translation in mammalian cells. J. Mol. Biol. 196:947-950.
7. Liu, L.F. 1989. DNA topoisomerase poisons as antitumor drugs. Annu. Rev. Biochem. 58:351-375.
8. Nelson, W.G., L.F. Liu and D.S. Coffey. 1986. Newly replicated DNA is associated with DNA topoisomerase II in cultured rat prostatic adenocarcinoma cells. Nature 322:187-189.
9. Nitiss, J.L. and W.T. Beck. 1996. Antitopoisomerase drug action and resistance. Eur. J. Cancer 6:958-966.
10. Tsai-Pflugfelder, M., L.F. Liu, A.A. Liu, K.M. Tewey, J. Whang-Peng, T. Knutsen, K. Huebner, C.M. Croce and J.C. Wang. 1988. Cloning and sequencing of cDNA encoding human DNA topoisomerase II and localization of the gene to chromosome region 17q21-22. Proc. Natl. Acad. Sci. USA 85:7177-7181.