Mutagenesis of key amino acids alters activity of a Saccharomyces cerevisiae endo-polygalacturonase expressed in Pichia pastoris

FEMS Microbiology Letters

Volumn 210, Issue 2, 2002, Pages 187-191

  Blanco, Pilar ^a   Thow, Graham ^a   Simpson, Craig G ^a   Villa, Tomas G ^b   Williamson, Brian ^a  

^a SCOTTISH CROP RESEARCH INSTITUTE   (United Kingdom)

^b UNIVERSITY OF SANTIAGO DE COMPOSTELA   (Spain)

Author keywords

Directed mutagenesis; Endo polygalacturonase; Pichia pastoris; Protein expression; Saccharomyces cerevisiae

Indexed keywords

AMINO ACID DERIVATIVE; ASPARTIC ACID; HISTIDINE; MATING HORMONE ALPHA FACTOR; MUTANT PROTEIN; POLYGALACTURONASE; SIGNAL PEPTIDE; METHANOL; RECOMBINANT PROTEIN;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME GLYCOSYLATION; ENZYME RELEASE; NONHUMAN; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN EXPRESSION; SACCHAROMYCES CEREVISIAE; SITE DIRECTED MUTAGENESIS; WILD TYPE; AMINO ACID SUBSTITUTION; ENZYMOLOGY; GENE VECTOR; GENETIC TRANSFORMATION; GENETICS; METABOLISM; PICHIA; SECRETION;

AMINO ACID SUBSTITUTION; GENETIC VECTORS; METHANOL; MUTAGENESIS, SITE-DIRECTED; PICHIA; POLYGALACTURONASE; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SUPPORT, NON-U.S. GOV'T; TRANSFORMATION, GENETIC;

PICHIA; PICHIA PASTORIS; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 0037035411     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(02)00634-1     Document Type: Article

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