Molecular cloning and expression of a polygalacturonase gene in Saccharomyces cerevisiae

Journal of Fermentation and Bioengineering

Volumn 86, Issue 3, 1998, Pages 332-334

  Hirose, Naoto ^a   Kishida, Masao ^a   Kawasaki, Haruhiko ^a   Sakai, Takuo ^b  

^a Osaka City University   (Japan)

^b KINKI UNIVERSITY   (Japan)

Author keywords

Cloning; Polygalacturonase; Saccharomyces cerevisiae

Indexed keywords

DNA SEQUENCES; ENZYMES; GENES; MOLECULES;

POLYGALACTURONASE; SACCHAROMYCES CEREVISIAE;

CLONING;

POLYGALACTURONASE;

AMINO ACID SEQUENCE; ARTICLE; DEPOLYMERIZATION; DNA TEMPLATE; ENZYME ACTIVE SITE; ENZYME PURIFICATION; GENE AMPLIFICATION; GENE EXPRESSION REGULATION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; SACCHAROMYCES CEREVISIAE;

SACCHAROMYCES CEREVISIAE;

EID: 0031686627     PISSN: 0922338X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0922-338X(98)80139-6     Document Type: Article

References (12)

1. Sakai, T., Sakamoto, T., Hallaert, J., and Vandamme, E. J.: Pectin, pectinase, and protopectinase: production, properties, and applications. Adv. Appl. Microbiol., 39, 213-294 (1993).
2. Sakai, T. and Okushima, M.: Purification and crystallization of a protopectin-solubilizing enzyme from Trichosporon penicil latum. Agric. Biol. Chem., 46, 667-676 (1978).
3. Sakai, T., Okushima, M., and Yoshitake, S.: Purification, crystallization and some properties of endopolygalacturonase from Kluyveromyces fragilis. Agric. Biol. Chem., 48, 1951-1961 (1984).
4. Yoshitake, S., Numata, T., Katsuragi, T., Hours, R. A., and Sakai, T.: Purification and characterization of pectin-releasing enzyme produced by Kluyveromyces wickerhamii. J. Ferment. Bioeng., 77, 370-375 (1994).
5. Iguchi, K., Kishida, M., and Sakai, T.: Purification and characterization of three extracellular protopectinases with polygalacturonase activities from Trichosporon penicillatum. Biosci. Biotech. Biochem., 60, 603-607 (1996).
6. Luh, B. S. and Phaff, H. J.: Studies on polygalacturonase of certain yeasts. Arch. Biochem. Biophys., 33, 212-227 (1951).
7. Gainvors, A., Frézier, V., Lemaresquier, H., Lequart, C., Aigel, M., and Belarbi, A.: Detection of polygalacturonase, pectin-lyase and pectin-esterase activities in a Saccharomyces cerevisiae strain. Yeast, 10, 1311-1319 (1994).
8. Julius, D., Brake, A., Blair, L., Kunisawa, R., and Thorner, J.: Isolation of the putative structural gene for lysine-arginine cleaving endopeptidase required for processing of yeast prepro-α-factor. Cell, 37, 1075-1089 (1984).
9. Wach, A., Pick, H., and Philippsen, P.: Procedures for isolating yeast DNA for different purposes, p. 1-16. In Johnston, J. R. (ed.), Molecular genetics of yeast: practical approach. IPL Press, Oxford (1994).
10. Mellor, J., Dobson, M. J., Roberts, N. A., Tuite, M. F., Emtage, J. S., White, S., Lowe, P. A., Patel, T., Kingsman, A. J., and Kingsman, S. M.: Efficient synthesis of enzymatically active calf chymosin in Saccharomyces cerevisiae. Gene, 24, 1-5 (1983).
11. Laemmli, U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685 (1970).
12. Iguchi, K., Hirano, H., Kishida, M., Kawasaki, H., and Sakai, T.: Cloning of a protopectinase gene of Trichosporon penicillatum and its expression in Saccharomyces cerevisiae. Microbiology, 143, 1657-1664 (1997).