1.68-Å crystal structure of endopolygalacturonase II from Aspergillus niger and identification of active site residues by site-directed mutagenesis

Journal of Biological Chemistry

Volumn 274, Issue 43, 1999, Pages 30474-30480

  Van Santen, Yovka ^a   Benen, Jacques A E ^b   Schröter, Klaus Hasso ^a,c   Kalk, Kor H ^a   Armand, Sylvie ^b   Visser, Jaap ^b   Dijkstra, Bauke W ^a  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

^b WAGENINGEN UNIVERSITY   (Netherlands)

^c ASTRAZENECA R AND D   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

POLYGALACTURONASE; ENDOPOLYGALACTURONASE II; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; ASPERGILLUS NIGER; CONFORMATIONAL TRANSITION; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME CONFORMATION; ENZYME SPECIFICITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN FOLDING; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION; AMINO ACID SUBSTITUTION; BINDING SITE; CHEMICAL STRUCTURE; CHEMISTRY; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; PROTEIN SECONDARY STRUCTURE; X RAY CRYSTALLOGRAPHY;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ASPERGILLUS NIGER; BINDING SITES; CRYSTALLOGRAPHY, X-RAY; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; POLYGALACTURONASE; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS;

EID: 0032589464     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.274.43.30474     Document Type: Article

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