A high-affinity Arg-X-X-Lys SH3 binding motif confers specificity for the interaction between Gads and SLP-76 in T cell signaling

Current Biology

Volumn 12, Issue 15, 2002, Pages 1336-1341

  Berry, Donna M ^a,b   Nash, Piers ^b,c   Liu, Stanley K W ^a,b   Pawson, Tony ^b,c   McGlade, C Jane ^a,b  

^a HOSPITAL FOR SICK CHILDREN   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

^c MOUNT SINAI HOSPITAL   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ARGININE; CARRIER PROTEIN; GRAP2 PROTEIN, HUMAN; GROWTH FACTOR RECEPTOR BOUND PROTEIN 2; INTERLEUKIN 2; LYMPHOCYTE ANTIGEN RECEPTOR; LYSINE; PEPTIDE FRAGMENT; PHOSPHOPROTEIN; PROTEIN; RECOMBINANT PROTEIN; SIGNAL TRANSDUCING ADAPTOR PROTEIN; SLP 76 SIGNAL TRANSDUCING ADAPTOR PROTEINS; SLP-76 SIGNAL TRANSDUCING ADAPTOR PROTEINS;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; BINDING SITE; CHEMISTRY; GENETIC TRANSCRIPTION; GENETICS; IMMUNOLOGY; KINETICS; METABOLISM; SITE DIRECTED MUTAGENESIS; SRC HOMOLOGY DOMAIN; T LYMPHOCYTE;

ADAPTOR PROTEINS, SIGNAL TRANSDUCING; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARGININE; BINDING SITES; CARRIER PROTEINS; GRB2 ADAPTOR PROTEIN; INTERLEUKIN-2; KINETICS; LYSINE; MUTAGENESIS, SITE-DIRECTED; PEPTIDE FRAGMENTS; PHOSPHOPROTEINS; PROTEINS; RECEPTORS, ANTIGEN, T-CELL; RECOMBINANT PROTEINS; SRC HOMOLOGY DOMAINS; T-LYMPHOCYTES; TRANSCRIPTION, GENETIC; SUPPORT, NON-U.S. GOV'T;

EID: 0037031154     PISSN: 09609822     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-9822(02)01038-2     Document Type: Article

References (19)

1. Adaptors and molecular scaffolds in immune cell signaling
2. Adapter proteins in lymphocyte antigen-receptor signaling
3. Protein-protein interactions define specificity in signal transduction
4. The hematopoietic-specific adaptor protein gads functions in T-cell signaling via interactions with the SLP-76 and LAT adaptors
5. GrpL, a Grb2-related adaptor protein, interacts with SLP-76 to regulate nuclear factor of activated T cell activation
6. Grf40, a novel grb2 family member, is involved in T cell signaling through interaction with SLP-76 and LAT
7. Requirement for the SLP-76 adaptor GADS in T cell development
8. Implication of the GRB2-associated phosphoprotein SLP-76 in T cell receptor-mediated interleukin 2 production
9. Spot-synthesis: An easy technique for positionally addressable, parallel chemical synthesis on a membrane support
10. Identification of an atypical Grb2 carboxyl-terminal SH3 domain binding site in Gab docking proteins reveals Grb2-dependent and -independent recruitment of Gab1 to receptor tyrosine kinases
11. The C-terminal SH3 domain of the adapter protein Grb2 binds with high affinity to sequences in Gab1 and SLP-76 which lack the SH3-typical P-x-x-P core motif
12. Molecular cloning and expression of human grap-2, a novel leukocyte-specific SH2- and SH3-containing adaptor-like protein that binds to gab-1
13. SPOT synthesis. Epitope analysis with arrays of synthetic peptides prepared on cellulose membranes
14. Differential requirement for SLP-76 domains in T cell development and function
15. Identification of a phospholipase C-gamma1 (PLC-gamma1) SH3 domain-binding site in SLP-76 required for T-cell receptor-mediated activation of PLC-gamma1 and NFAT
16. Coupling of Gab1 to c-Met, Grb2, and Shp2 mediates biological responses
17. A deubiquitinating enzyme UBPY interacts with the Src homology 3 domain of Hrs-binding protein via a novel binding motif PX(V/I)(D/N) RXXKP
18. Multisite phosphorylation of a CDK inhibitor sets a threshold for the onset of DNA replication
19. The Shc adaptor protein is highly phosphorylated at conserved, twin tyrosine residues (Y239/240) that mediate protein-protein interactions