Development of a PCR-compatible enrichment medium for Yersinia enterocolitica: Amplification precision and dynamic detection range during cultivation

International Journal of Food Microbiology

Volumn 72, Issue 3, 2002, Pages 185-201

  Knutsson, Rickard ^a   Fontanesi, Massimo ^a   Grage, Halfdan ^a   Rådström, Peter ^a  

^a LUND UNIVERSITY   (Sweden)

Author keywords

Amplification efficiency; Dynamic detection range; Experimental design; PCR inhibitors; Real time PCR; Sample preparation; Yersinia enterocolitica; YPCE medium

Indexed keywords

FLUORESCENT DYE;

ARTICLE; BACTERIUM CULTURE; COLONY FORMING UNIT; CONTROLLED STUDY; CULTURE MEDIUM; EVALUATION; FLORA; GENE AMPLIFICATION; INOCULATION; NONHUMAN; POLYMERASE CHAIN REACTION; SCREENING; YERSINIA ENTEROCOLITICA;

BUFFERS; COLONY COUNT, MICROBIAL; CULTURE MEDIA; DNA, BACTERIAL; FOOD MICROBIOLOGY; NUCLEIC ACID AMPLIFICATION TECHNIQUES; POLYMERASE CHAIN REACTION; YERSINIA ENTEROCOLITICA;

YERSINIA; YERSINIA ENTEROCOLITICA;

EID: 0037022311     PISSN: 01681605     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1605(01)00636-5     Document Type: Article

References (43)

1. Capacity of nine thermostable DNA polymerases to mediate DNA amplification in the presence of PCR-inhibiting samples
2. Effects of amplification facilitators on diagnostic PCR in the presence of blood, feces, and meat
3. Purification and characterization of PCR-inhibitory components in blood cells
4. Alkali method for rapid recovery of Yersinia enterocolitica and Yersinia pseudotuberculosis from foods
5. Molecular diagnostics for dairy-borne pathogens
6. PCR bias in ecological analysis: A case study for quantitative Taq nuclease assays in analyses of microbial communities
7. A new isolation for the recovery of Yersinia enterocolitica from environmental sources
8. A simplified sample preparation method from various foods for PCR detection of pathogenic Yersinia enterocolitica: A possible model for other food pathogens
9. Development of real-time PCR assays for rapid detection of Pfiesteria piscicida and related dinoflagellates
10. A mathematical approach toward defining and calculating the duration of the lag phase
11. Isolation of Yersinia enterocolitica from foods
12. Shortcomings of irgasan ticarcillin chlorate broth for the enrichment of Yersinia enterocolitica biotype 2, serotype 9 from meat
13. Microorganisms in food ecosystems
14. Inhibition of Yersinia enterocolitica serotype O3 by natural microflora of pork
15. Automated 5′ nuclease PCR assay for identification of Salmonella enterica
16. Development of a fluorogenic 5′ nuclease PCR assay for detection of the ail gene of pathogenic Yersinia enterocolitica
17. Proviral load determination of different feline immunodeficiency virus isolates using real-time polymerase chain reaction: Influence of mismatches on quantification
18. Detection of pathogenic Yersinia enterocolitica in enrichment media and pork by a multiplex PCR: A study of sample preparation and PCR-inhibitory components
19. Biotechnical use of polymerase chain reaction for microbiological analysis of biological samples
20. Effect of macromolecular crowding upon the structure and function of an enzyme: Glyceraldehyde-3-phosphate dehydrogenase
21. Nordic committee on food analysis NMKL-163: Pathogenic Yersinia enterocolitica
22. Application of the 5′ -nuclease PCR assay in evaluation and development of methods for quantitative detection of Campylobacter jejuni
23. PCR-based DNA amplification and presumptive detection of Escherichia coli O157:H7 with an internal fluorogenic probe and the 5′ nuclease (TaqMan) assay
24. Product differentiation by analysis of DNA melting curves during the polymerase chain reaction
25. Inhibition of PCR by components of food samples, microbial diagnostic assays and DNA-extraction solutions
26. Synthesis of a selective agar medium for Yersinia enterocolitica
27. Yersinia enterocolitica: Observations on some growth characteristics and response to selective agents
28. Alkalotolerance of Yersinia enterocolitica as a basis for selective isolation from food enrichments
29. Antagonism by Gram-negative bacteria to growth of Yersinia enterocolitica in mixed cultures
30. Quantitative reverse transcription-polymerase chain reaction to study mRNA decay: Comparison of endpoint and real-time methods
31. Enzymological considerations for a theoretical description of the quantitative competitive polymerase chain reaction (QC-PCR)
32. Preparing samples for rapid detection of microbes
33. Evaluation of analytical data
34. Evaluation of different antimicrobial agents used as selective supplements for isolation and recovery of Yersinia enterocolitica
35. Rapid 5′ nuclease (TaqMan) assay for detection of virulent strains of Yersinia enterocolitica
36. New enrichment method for isolation of pathogenic Yersinia enterocolitica serogroup O:3 from pork
37. Influence of DNA target melting behaviour on real-time PCR quantification
38. Continuous fluorescence monitoring of rapid cycle DNA amplification
39. Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) for measurement of cytokine and growth factor mRNA expression with fluorogenic probes or SYBR green I
40. Macromolecular crowding extends the range of conditions under which DNA polymerase is functional