Disassembly of interchromatin granule clusters alters the coordination of transcription and pre-mRNA splicing

Journal of Cell Biology

Volumn 156, Issue 3, 2002, Pages 425-436

  Sacco Bubulya, Paula ^a   Spector, David L ^a  

^a Howard Hughes Medical Institute Cold Spring Harbor Laboratory Cold Spring Harbor   (United States)

Author keywords

Clk STY; Interchromatin granule cluster; Nucleus; Pre mRNA splicing; Transcription

Indexed keywords

CDC2 LIKE KINASE; MESSENGER RNA PRECURSOR; PROTEIN KINASE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL GRANULE; CHROMATIN STRUCTURE; CONTROLLED STUDY; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN PROTEIN INTERACTION; RNA SPLICING; TRANSCRIPTION REGULATION;

CELL NUCLEUS; CELLS, CULTURED; CHROMATIN; CYTOPLASMIC GRANULES; CYTOSKELETON; FLUORESCENT ANTIBODY TECHNIQUE; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GREEN FLUORESCENT PROTEINS; HUMANS; LUMINESCENT PROTEINS; MICROSCOPY, ELECTRON; MUTATION; PROTEIN-SERINE-THREONINE KINASES; PROTEIN-TYROSINE KINASES; RECOMBINANT FUSION PROTEINS; RNA SPLICING; RNA, MESSENGER; TRANSCRIPTION, GENETIC;

ANIMALIA; MAMMALIA;

EID: 0037017406     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.200107017     Document Type: Article

References (57)

1. The fission yeast prp4+ gene involved in pre-mRNA splicing codes for a predicted serine/threonine kinase and is essential for growth
2. A mammalian protein kinase with potential for serine/threonine and tyrosine phosphorylation is related to cell cycle regulators
3. A new staining procedure for electron microscopical cytology
4. Phosphoinositide signaling pathways in nuclei are associated with nuclear speckles containing pre-mRNA processing factors
5. Evidence that "pinin", reportedly a differentiation-specific desmosomal protein, is actually a widespread nuclear protein
6. Transcription-dependent redistribution of the large subunit of RNA polymerase II to discrete nuclear domains
7. Transcription-dependent colocalization of the U1, U2, U4/U6 and U5 sn-RNPs in coiled bodies
8. Ultrastructural analysis of transcription and splicing in the cell nucleus after bromo-UTP microinjection
9. SRPK1 and Clk/Sty protein kinases show distinct substrate specificities for serine/arginine-rich splicing factors
10. The Clk/Sty protein kinase phosphorylates splicing factors and regulates their intranuclear distribution
11. In vivo regulation of alternative pre-mRNA splicing by the Clk1 protein kinase
12. Quantitative imaging of pre-mRNA splicing factors in living cells
13. Perichromatin fibrils are in situ forms of nascent transcripts
14. The ultrastructural visualization of nucleolar and extranucleolar RNA synthesis and distribution
15. Cajal bodies: The first 100 years
16. Assembly of the nuclear transcription and processing machinery: Cajal bodies (coiled bodies) and transcriptosomes
17. A serine kinase regulates intracellular localization of splicing factors in the cell cycle
18. Mapping of B cell epitopes on small nuclear ribonucleoproteins that react with human autoantibodies as well as with experimentally induced mouse monoclonal antibodies
19. Characterization by cDNA cloning of two new human protein kinases. Evidence by sequence comparison of a new family of mammalian protein kinases
20. Effects of fibronectin fragments on DNA transfection into mammalian cells by electroporation
21. A human common nuclear matrix protein homologous to eukaryotic translation initiation factor 4A
22. STY, a tyrosine-phosphorylating enzyme with sequence homology to serine/threonine kinases
23. In vivo analysis of the stability and transport of nuclear poly(A)+ RNA
24. Intron-dependent recruitment of pre-mRNA splicing factors to sites of transcription
25. Colocalization ofintranuclear lamin foci with RNA splicing factors
26. In vivo evidence that transcription and splicing are coordinated by a recruiting mechanism
27. Molecular cloning of a novel human cdc2/CDC28-like protein kinase
28. Cloning of human PRP4 reveals interaction with Clk1
29. Reduced mobility of the alternate splicing factor (ASF) through the nucleoplasm and steady state speckle compartments
30. Structure and function in the nucleus
31. Activity and autophosphorylation of LAMMER protein kinases
32. Eukaryotic translation initiation factor 4AIII (eIF4AIII) is functionally distinct from eIF4AI and eIF4AII
33. Dual-specificity protein kinases: Will any hydroxl do?
34. Purification and biochemical characterization of interchromatin granule clusters
35. The dynamics of a pre-mRNA splicing factor in living cells
36. Small nuclear RNA-protein complex anchors on the actin filaments in bovine lymphocyte nuclear matrix
37. Characterization and comparison of four serine- and arginine-rich (SR) protein kinases
38. Disruption of pre-mRNA splicing in-vivo results in reorganization of splicing factors
39. Antibodies differentiate desmosome-form and nucleus-form pinin: Evidence that pinin is a moonlighting protein with dual location at the desmosome and within the nucleus
40. Characterization of pinin, a novel protein associated with the desmosome-intermediate filament complex
41. Cloning and analysis of cDNA encoding murine pinin
42. Actin bound to the heterogeneous nuclear ribonucleoprotein hrp36 is associated with balbiani ring mRNA from the gene to polysomes
43. High mobility of proteins in the mammalian cell nucleus
44. The protein kinase Clk/Sty directly modulates SR protein activity: Both hyper- and hypophosphorylation inhibit splicing
45. A monoclonal antibody that recognizes a phosphorylated epitope stains lampbrush chromosomes and small granules in the amphibian germinal vesicle
46. Higher order nuclear organization: Three-dimensional distribution of small nuclear ribonucleoprotein particles
47. Differences in snRNP localization between transformed and nontransformed cells
48. Nuclear organization of pre-mRNA processing
49. Dynamics of transcription and pre-mRNA splicing within the mammalian cell nucleus
50. Visualization of gene activity in living cells
51. Identification of protein antigens associated with the nuclear matrix and with clusters of interchromatin granules in both interphase and mitotic cells
52. SRPK2: A differentially expressed SR protein-specific kinase involved in mediating the interaction and localization of pre-mRNA splicing factors in mammalian cells
53. Three-dimensional visualization of transcription sites and their association with splicing factor-rich nuclear speckles
54. Epidermal induction and inhibition of neural fate by translation initiation factor 4AIII
55. Higher level organization of individual gene transcription and RNA splicing
56. Phosphorylation regulates in vivo interaction and molecular targeting of serine/arginine-rich pre-mRNA splicing factors
57. Rapid and phosphoinositol-dependent binding of the SWI/SNF-like BAF complex to chromatin after T lymphocyte receptor signaling