Heparinase I from Flavobacterium heparinum. Identification of a critical histidine residue essential for catalysis as probed by chemical modification and site-directed mutagenesis

Biochemistry

Volumn 35, Issue 21, 1996, Pages 6846-6852

  Godavarti, Ranga ^a   Cooney, Charles L ^a   Langer, Robert ^a,b   Sasisekharan, Ram ^b  

^a USA   (United States)

^b MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HEPARIN LYASE; HYDROXYLAMINE;

ARTICLE; BINDING SITE; CONFORMATIONAL TRANSITION; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME ISOLATION; FLAVOBACTERIUM; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION;

AMINO ACID SEQUENCE; BASE SEQUENCE; DIETHYL PYROCARBONATE; DNA PRIMERS; FLAVOBACTERIUM; HEPARIN; HEPARIN LYASE; HISTIDINE; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; OLIGOSACCHARIDES; POINT MUTATION; POLYMERASE CHAIN REACTION; POLYSACCHARIDE-LYASES; RECOMBINANT PROTEINS;

FLAVOBACTERIUM; PEDOBACTER HEPARINUS;

EID: 0029894786     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi960356g     Document Type: Article

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