Overexpression of glutathione S-transferase A1-1 in ECV 304 cells protects against busulfan mediated G2-arrest and induces tissue factor expression

British Journal of Pharmacology

Volumn 137, Issue 7, 2002, Pages 1100-1106

  Ritter, Christoph A ^a,b   Sperker, Bernhard ^a   Grube, Markus ^a   Dressel, Dana ^a   Kunert Keil, Christiane ^a   Kroemer, Heyo K ^a  

^a UNIVERSITY OF GREIFSWALD   (Germany)

^b VANDERBILT UNIVERSITY   (United States)

Author keywords

Busulfan; ECV 304; G2 arrest; GST A1 1; Tissue factor

Indexed keywords

BUSULFAN; COMPLEMENTARY DNA; GLUTATHIONE TRANSFERASE; ISOENZYME; PROTEIN KINASE; TETRAHYDROTHIOPHENE; THIOPHENE DERIVATIVE; THROMBOPLASTIN; UNCLASSIFIED DRUG; GLUTATHIONE S-TRANSFERASE ALPHA; GLUTATHIONE TRANSFERASE ALPHA;

ARTICLE; BIOLOGICAL MODEL; CELL CYCLE G2 PHASE; CELL LINE; CELL PROTECTION; COMPARATIVE STUDY; DRUG EFFECT; DRUG METABOLISM; ENZYME ACTIVITY; GENE OVEREXPRESSION; GENETIC TRANSFECTION; MITOSIS INHIBITION; PRIORITY JOURNAL; PROTEIN EXPRESSION; BIOSYNTHESIS; CELL CYCLE; CELL DIVISION; DOSE RESPONSE; GENETICS; HUMAN; METABOLISM; PLASMID; TIME; WESTERN BLOTTING;

BLOTTING, WESTERN; BUSULFAN; CELL CYCLE; CELL DIVISION; CELL LINE; DNA, COMPLEMENTARY; DOSE-RESPONSE RELATIONSHIP, DRUG; G2 PHASE; GLUTATHIONE TRANSFERASE; HUMANS; ISOENZYMES; PLASMIDS; THIOPHENES; THROMBOPLASTIN; TIME FACTORS; TRANSFECTION;

EID: 0036899908     PISSN: 00071188     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.bjp.0704972     Document Type: Article

References (46)

1. Armesilla, A.L., Lorenzo, E., Gomez del Arco, P., Martinez-Martinez, S., Alfranca, A. & Redondo, J.M. (1999). Vascular endothelial growth factor activates nuclear factor of activated T cells in human endothelial cells: A role for tissue factor gene expression. Mol. Cell. Biol., 19, 2032-2043.
2. Asada, Y., Marutsuka, K., Hatakeyama, K., Sato, Y., Hara, S., Kisanuki, A. & Sumiyoshi, A. (1998). The role of tissue factor in the pathogenesis of thrombosis and atherosclerosis. J. Atheroscler. Throm., 4, 135-139.
3. Bearman, S.I. (1995). The syndrome of hepatic veno-occlusive disease after marrow transplantation. Blood, 85, 3005-3020.
4. Board, P.G., Baker, R.T., Chelvanayagam, G. & Jermiin, L.S. (1997). Zeta, a novel class of glutathione transferases in a range of species from plants to humans. Biochem. J., 328, 929-935.
5. Board, P.G., Coggan, M., Chelvanayagam, G., Easteal, S., Jermiin, L.S., Schulte, G.K., Danley, D.E., Hoth, L.R., Griffor, M.C., Kamath, A.V., Rosner, M.H., Chrunyk, B.A., Perregaux, D.E., Gabel, C.A., Geoghegen, K.F. & Pandit, J. (2000). Identification, characterization, and crystal structure of the Omega class glutathione transferases. J. Biol. Chem., 275, 24798-24806.
6. Bredschneider, M., Klein, K., Murdter, T.E., Marx, C., Eichelbaum, M., Nussler, A.K., Neuhaus, P., Zanger, U.M. & Schwab, M. (2002). Genetic polymorphisms of glutathione S-transferase A1, the major glutathione S-transferase in human liver: Consequences for enzyme expression and busulfan conjugation. Clin. Pharmacol. Ther., 71, 479-487.
7. Chen, N.G., Sarabia, S.F., Malloy, P.J., Zhao, X.Y., Feldman, D. & Reaven, G.M. (1999). PPARgamma agonists enhance human vascular endothelial adhesiveness by increasing ICMA-1 expression. Biochem. Biophys. Res. Commun., 263, 718-722.
8. Cnubben, N.H., Rommens, A.J., Oudshoorn, M.J. & Van Bladeren, P.J. (1998). Glutathione-dependent biotransformation of the alkylating drug thiotepa and transport of its metabolite monoglutathionylthiotepa in human MCF-7 breast cancer cells. Cancer Res., 58, 4616-4623.
9. Coles, B.F., Morel, F., Rauch, C., Huber, W.W., Yang, M., Teitel, C.H., Green, B., Lang, N.P. & Kadlubar, F.F. (2001). Effect of polymorphism in the human glutathione S-transferase A1 promoter on hepatic GSTA1 and GSTA2 expression. Pharmacogenetics, 11, 663-669.
10. Czerwinski, M., Gibbs, J.P. & Slattery, J.T. (1996). Busulfan conjugation by Glutathione S-Transferases alpha, mu, and pi. Drug Metab. Dispos., 24, 1015-1019.
11. Demagalhaes Silverman, M., Lister, J., Rybka, W., Wilson, J. & Ball, E. (1997). Busulfan and cyclophosphamide (BU/CY2) as preparative regimen for patients with lymphoma. Bone Marrow Transplant, 19, 777-781.
12. Dirven, H.A., Dictus, E.L., Broeders, N.L., Van Ommen, B. & Van Bladeren, P.J. (1995). The role of human glutathione S-transferase isoenzymes in the formation of glutathione conjugates of the alkylating cytostatic drug thiotepa. Cancer Res., 55, 1701-1706.
13. Dix, S.P., Wingard, J.R., Mullins, R.E., Jerkunica, I., Davidson, T.G., Gilmore, C.E., York, R.C., Lin, L.S., Devine, S.M., Geller, R.B., Heffner, L.T., Hillyer, C.D., Holland, H.K., Winton, E.F. & Saral, R. (1996). Association of busulfan area under the curve with veno-occlusive disease following BMT. Bone Marrow Transplant, 17, 225-230.
14. Ehrsson, H., Hassan, M., Ehrnebo, M. & Beran, M. (1983). Busulfan kinetics. Clin. Pharmacol. Ther., 34, 86-89.
15. Gibbs, J.P., Czerwinski, M. & Slattery, J.T. (1996). Busulfan-glutathione conjugation catalysed by human liver cytosolic glutathione S-transferases. Cancer Res., 56, 3678-3681.
16. Giorgiani, G., Bozzola, M., Locatelli, F., Picco, P., Zecca, M., Cisternino, M., Dallorso, S., Bonetti, F., Dini, G., Borrone, C., Regazzi, M.B., De Stefano, P. & Severi, F. (1995). Role of busulfan and total body irradiation on growth of prepubertal children receiving bone marrow transplantation and results of treatment with recombinant human growth hormone. Blood, 86, 825-831.
17. Grochow, L.B., Jones, R.J., Brundrett, R.B., Braine, H.G., Chen, T.L., Saral, R., Santos, G.W. & Colvin, O.M. (1989). Pharmacokinetics of busulfan: Correlation with veno-occlusive disease in patients undergoing bone marrow transplantation. Cancer Chemlther. Pharmacol., 25, 55-61.
18. Haller, C., Kiessling, F. & Kubler, W. (1998). Polarized expression of heterologous membrane proteins transfected in a human endothelial-derived cell line. Eur. J. Cell. Biol., 75, 353-361.
19. Hansen, J.B., Svensson, B., Olsen, R., Ezban, M., Osterud, B. & Paulssen, R.H. (2000). Heparin induces synthesis and secretion of tissue factor pathway inhibitor from endothelial cells in vitro. Thromb. Haemost., 83, 973-943.
20. Harlow, E. & Lane, D. (1988). Immunoblotting protocols. In: Antibodies: A laboratory manual. ed. Harlow, E., Lane, D. pp. 479-510. Cold Spring Harbor: Cold Spring Harbor Laboratory Press.
21. Hassan, M. & Ehrsson, H. (1987a). Urinary metabolites of busulfan in the rat. Drug Metab. Dispos., 15, 399-402.
22. Hassan, M. & Ehrsson, H. (1987b). Metabolism of 14C-busulfan in isolated perfused rat liver. Eur. J. Drug Metab. Pharmacokinet., 12, 71-76.
23. Hayes, J.D., Kerr, L.A. & Cronshaw, A.D. (1989). Evidence that glutathione S-transferase B1B1 and B2B2 are the products of separate genes and that their expression in human liver is subject to inter-individual variation. Biochem., 264, 437-445.
24. Hayes, J.D. & Pulford, D.J. (1995). The glutathione S-transferase supergene family: Regulation of GST* and the contribution of the isoenzymes to cancer chemoprotection and drug resistance. Crit. Rev. Biochem. Mol. Biol., 30, 445-598.
25. Hughes, S.E. (1996). Functional characterization of the spontaneously transformed human umbilical vein endothelial cell line ECV304: Use in an in vitro model of angiogenesis. Exp. Cell. Res., 225, 171-185.
26. Iguchi, A., Kobayashi, R., Yoshida, M., Kobayashi, K., Matsuo, K., Kitajima, I. & Maruyama, I. (2001). Vascular endothelial growth factor (VEGF) is one of the cytokines causative and predictive of hepatic veno-occlusive disease (VOD) in stem cell transplantation. Bone Marrow Transplant., 27, 1173-1180.
27. Inskeep, P.B. & Guengerich, F.P. (1984). Glutathione-mediated binding of dibromoalkanes to DNA: Specificity of rat glutathione-S-transferases and dibromoalkane structure. Carcinogenesis, 5, 805-808.
28. Khalil, A., Ciobanu, N., Sparano, J.A., Gucalp, R., Dutcher, J.P. & Wiernik, P.H. (1995). Pilot study of high-dose mitoxantrone and busulfan plus autologous bone transplantation in patients with advanced malignancies. Bone Marrow Transplant., 15, 93-97.
29. Kirchhofer, D. & Nemerson, Y. (1996). Initiation of blood coagulation: The tissue factor/factor VIIa complex. Curr. Opin. Biotechnol., 7, 386-391.
30. Lopez-Pedrera, C., Jardi, M., Ingles-Esteve, J., Munoz-Canoves, P., Dorado, G., Velasco, F. & Felez, J. (1997). Characterization of tissue factor expression on the human endothelial cell line ECV304. Am. J. Hematol., 56, 71-78.
31. Millar, B.C., Tilby, M.J., Ormerod, M.G., Payne, A.W.R., Jinks, S. & Loverock, P.S. (1986). Comparative studies of total cross-linking, cell survival and cell cycle perturbations in chinese hamster cells treated with alkylating agents in vitro. Biochem. Pharmacol., 35, 1163-1169.
32. Olavarria, E., Kanfer, E., Szydlo, R., O'Brien, S., Craddock, C., Apperley, J. & Goldman, J. (2000). High-dose busulfan alone as cytoreduction before allogeneic or autologous stem cell transplantation for chronic myeloid leukaemia: A single-centre experience. Br. J. Haematol., 108, 769-777.
33. Paumi, C.M., Ledford, B.G., Smitherman, P.K., Townsend, A.J. & Morrow, C.S. (2001). Role of multidrug resistance protein 1 (MRP1) and glutathione S-transferase A1-1 in alkylating agent resistance. Kinetics of glutathione conjugate formation and efflux govern differential cellular sensitivity to chlorambucil versus melphalan toxicity. J. Biol. Chem., 276, 7952-7956.
34. Qu, J., Conroy, L.A., Walker, J.H., Wooding, F.B. & Lucy, J.A. (1996). Phosphatidylserine-mediated adhesion of T-cells to endothelial cells. Biochem. J., 317 (Pt 2), 343-346.
35. Ritter, C.A., Bohnenstengel, F., Hofmann, U., Kroember, H.K. & Sperker, B. (1999). Determination of tetrahydrothiophene formation as a probe of in vitro busulfan metabolism by human glutathione S-transferase A1-1: Use of a highly sensitive gas chromatographic-mass spectrometric method. J. Chromatogr. B Biomed. Sci. Appl., 730, 25-31.
36. Rozman, C., Carreras, E., Qian, C., Gale, R.P., Bortin, M.M., Rowlings, P.A., Ash, R.C., Champlin, R.E., Henslee Downey, P.J., Herzig, R.H., Hinterberger, W., Klein, J.P., Prentice, H.G., Reiffers, J., Zwaan, F.E. & Horowitz, M.M. (1996). Risk factors for hepatic veno-occlusive disease following HLA-identical sibling bone marrow transplants for leukemia. Bone Marrow Transplant, 17, 75-80.
37. Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989). Molecular Cloning - A Laboratory Manual. Cold Spring Harbor: Cold Spring Harbor Laboratory Press.
38. Schiffman, K., Buckner, C.D., Maziarz, R., Maloney, D.G., Appelbaum, F.R., Press, O., Gooley, T., Holmberg, L., Lilleby, K., Clift, R., Zuckerman, N., Klarnet, J., Weaver, C., Chauncey, T. & Bensinger, W.I. (1997). High-dose busulfan, melphalan, and thiotepa followed by autologous peripheral blood stem cell transplantation in patients with aggressive lymphoma or relapsed Hodgkin's disease. Biol. Blood Marrow Transplant., 3, 261-266.
39. Schuler, U.S., Ehrsam, M., Schneider, A., Schmidt, H., Deeg, J. & Ehninger, G. (1998). Pharmacokinetics of intravenous busulfan and evaluation of the bioavailability of the oral formulation in conditioning for haematopoietic stem cell transplantation. Bone Marrow Transplant., 22, 241-244.
40. Smith, P.K., Krohn, R.I., Hermanson, G.T., Mallia, A.K., Gartner, F.H., Provenzano, M.D., Fujimoto, E.K., Goeke, N.M., Olson, B.J. & Klenk, D.C. (1985). Measurement of protein using bicinchoninic acid. Anal. Biochem., 150, 76-85.
41. Takahashi, K., Sawasaki, Y., Hata, J.-I., Mukai, K. & Goto, T. (1990). Spontaneous transformation and immortalization of human endothelial cells. In Vitro Cell Dev. Biol. 25, 265-274.
42. Takahashi, K. & Sawasaki, Y. (1991). Human endothelial cell line, ECV304, produces pro-urokinase. In Vitro Cell. Dev. Biol., 27A, 766-768.
43. Tong, W.P. & Ludlum, D.B. (1980). Crosslinking of DNA by busulfan. Formation of diguanyl derivatives. Biochim. Biophys. Acta, 608, 174-181.
44. Vassal, G., Koscielny, S., Challine, D., Valteau Couanet, D., Boland, I., Deroussent, A., Lemerle, J., Gouyette, A. & Hartmann, O. (1996). Busulfan disposition and hepatic venoocclusive disease in children undergoing bone marrow transplantation. Cancer Chemother. Pharmacol., 37, 247-253.
45. Villarete. L.H. & Remick, D.G. (1995). Nitric oxide regulation of IL-8 expression in human endothelial cells. Biochem. Biophys. Res. Commun., 211, 671-676.
46. Zander, A.R., Berger, C., Kröger, N., Stockschläder, M., Krüger, W., Horstmann, M., Grimm, J., Zeller, W., Kabisch, H., Erttmann, R., Schönrock, P., Kuse, R., Braumann, D., Illiger, H.-J., Fiedler, W., De Witt, M., Hossfeld, K.D. & Weh, H.-J. (1997). High dose chemotherapy with busulfan, cyclophosphamide, and etoposide as conditioning regimen for allogeneic bone marrow transplantation for patients with acute myeloid leukemia in first complete remission. Clin. Cancer Res., 3, 2671-2675.