Polarized expression of heterologous membrane proteins transfected in a human endothelial-derived cell line

European Journal of Cell Biology

Volumn 75, Issue 4, 1998, Pages 353-361

  Haller, Christlieb ^a,b   Kiessling, Fabian ^a   Kübler, Wolfgang ^a  

^a UNIVERSITY OF HEIDELBERG   (Germany)

^b UNIVERSITY HOSPITAL HEIDELBERG   (Germany)

Author keywords

CD7; ECV304; Endothelial polarity; Influenza hemagglutinin; Uvomorulin

Indexed keywords

CADHERIN; CALCIUM PHOSPHATE; CD7 ANTIGEN; CELL MARKER; CELL SURFACE PROTEIN; COMPLEMENTARY DNA; HEMAGGLUTININ; LYMPHOCYTE SURFACE MARKER; MEMBRANE PROTEIN; UVOMORULIN;

ARTICLE; CELL CLONE; CELL IMMORTALIZATION; CELL MEMBRANE TRANSPORT; CELL POLARITY; CELL STRAIN; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; DNA TRANSFECTION; ENDOTHELIUM CELL; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IMMUNOBLOTTING; IMMUNOELECTRON MICROSCOPY; IMMUNOFLUORESCENCE MICROSCOPY; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN TARGETING; PROTEIN TRANSPORT;

EID: 0031977014     PISSN: 01719335     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0171-9335(98)80068-X     Document Type: Article

References (34)

1. Abedi, H., I. Zachary: Vascular endothelial growth factor stimulates tyrosine phosphorylation and recruitment to new focal adhesion of focal adhesion kinase and paxillin in endothelial cells. J. Biol. Chem. 272, 15442-15451 (1997).
2. Aruffo, A., B. Seed: Molecular cloning of two CD7 (T-cell leukemia antigen) cDNAs by a COS cell expression system. EMBO J. 6, 3313-3316 (1987).
3. Ayalon, O., H. Sabanai, M.-G. Lampugnani, E. Dejana, B. Geiger: Spatial and temporal relationships between cadherins and PECAM-1 in cell-cell junctions of human endothelial cells. J. Cell Biol. 126, 247-258 (1994).
4. Bacallao, R., E. H. Stelzer: Preservation of biological specimens for observation in a confocal fluorescence microscope and operational principles of confocal fluorescence microscopy. Methods Cell Biol. 31, 437-452 (1989).
5. Boller, K., D. Vestweber, R. Kemler: Cell-adhesion molecule uvomorulin is localized in the intermediate junctions of adult intestinal epithelial cells. J. Cell Biol. 100, 327-332 (1985).
6. Brewer, C. B., M. G. Roth: A single amino acid change in the cytoplasmic domain alters the polarized delivery of influenza virus hemagglutinin. J. Cell Biol. 114, 413-421 (1991).
7. Camerer, E., S. Pringle, A. H. Skartlien, M. Wiiger, K. Prydz, A. B. Kolsto, H. Prydz: Opposite sorting of tissue factor in human umbilical vein endothelial cells and Madin-Darby canine kidney epithelial cells. Blood 88, 1339-1349 (1996).
8. Drubin, D. G., W. J. Nelson: Origins of cell polarity. Cell 84, 335-344 (1996).
9. Eaton, S., K. Simons: Apical, basal, and lateral cues for epithelial polarization. Cell 82, 1-20 (1995).
10. Franke, W. W., M. R. Lüder, J. Kartenbeck, H. Zerban, T. W. Keenan: Involvement of vesicle coat material in casein secretion and surface regeneration. J. Cell Biol. 69, 173-195 (1976).
11. Gumbiner, B.: Generation and maintenance of epithelial cell polarity. Curr. Opin. Cell Biol. 2, 881-887 (1990).
12. Haller, C., S. L. Alper: Non-polarized surface distribution and delivery of human CD7 in polarized MDCK cells. Am. J. Physiol. 265, C1069-1079 (1993).
13. Haller, C., S. L. Alper: Assessment of heterologous membrane protein polarity in transiently transfected MDCK cells. Cytotechnology 17, 71-82 (1995).
14. Hughes, S. E.: Functional characterization of the spontaneously transformed human umbilical vein endothelial cell line ECV304: use in an in vitro model of angiogenesis. Exp. Cell Res. 225, 171-185 (1996).
15. Jaffe, S., P. Oliver, S. Farooqui, P. Novak, N. Sorgente, V. Kalra: Separation of luminal and abluminal membrane enriched domains from cultured bovine aortic endothelial cells: monoclonal antibodies specific for endothelial cell plasma membranes. Biochim. Biophys. Acta 898, 37-52 (1987).
16. Kikkawa, Y., K. Akaogi, H. Mizushima, N. Yamanaka, M. Umeda, K. Miyazaki: Stimulation of endothelial cell migration in culture by ladsin, a laminin-5-like cell adhesion protein. In Vitro Cell Dev. Biol. Anim. 32, 46-52 (1996).
17. Lawrie, A. M., R. Rizzuto, T. Pozzan, A. W. Simpson: A role for calcium influx in the regulation of mitochondrial calcium in endothelial cells. J. Biol. Chem. 271, 10753-10759 (1996).
18. Lazarovits, J., H. Y. Naim, A. C. Rodriguez, R. H. Wang, E. Fire, C. Bird, Y. I. Henis, M. G. Roth: Endocytosis of chimeric influenza virus hemagglutinin proteins that lack a cytoplasmic recognition feature for coated pits. J. Cell Biol. 134, 339-348 (1996).
19. Mellman, I., E. Yamamoto, J. A. Whitney, M. Kim, W. Hunziker, K. Matter: Molecular sorting in polarized and non-polarized cells: common problems, common solutions. J. Cell. Sci. Suppl. 17, 1-7 (1993).
20. Muller, W., M. Gimbrone: Plasmalemmal proteins of cultured vascular endothelial cells exhibit apical-basal polarity: analysis by surface-selective iodination. J. Cell Biol. 103, 2389-2404 (1986).
21. Narahara, N., T. Enden, M. Wiiger, H. Prydz: Polar expression of tissue factor in human umbilical vein endothelial cells. Arterioscler. Thromb. 14, 1815-1820 (1994).
22. Nelson, J.: Renal epithelial cell polarity. Curr. Opin. Nephrol. Hypertens. 1, 59-67 (1992).
23. Rodriguez-Boulan, E., J. Nelson: Morphogenesis of the polarized epithelial cell phenotype. Science 245, 718-725 (1989).
24. Rodriguez-Boulan, E., C. Zurzolo: Polarity signals in epithelial cells. J. Cell Sci. Suppl. 17, 9-12 (1993).
25. Roth, M. G., J. P. Fitzpatrick, R. W. Compans: Polarity of influenza and vesicular stomatitis virus maturation in MDCK cells: lack of a requirement for glycosylation of viral proteins. Proc. Natl. Acad. Sci. USA 76, 6430-6434 (1979).
26. Sambrook, J., E. F. Fritsch, T. Maniatis: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press. Cold Spring Harbor, NY 1989.
27. Schuh, R., D. Vestweber, I. Riede, M. Ringwald, U. B. Rosenberg, H. Jäckle, R. Kemler: Molecular cloning of the mouse cell adhesion molecule uvomorulin: cDNA contains a B1-related sequence. Proc. Natl. Acad. Sci. USA 83, 1364-1368 (1986).
28. Simons, K., S. D. Fuller: Cell surface polarity in epithelia. Annu. Rev. Cell Biol. 1, 243-288 (1985).
29. Sporn, L., V. Marder, D. Wagner: Differing polarity of the constitutive and regulated secretory pathways for von Willebrand factor in endothelial cells. J. Cell Biol. 108, 1283-1289 (1989).
30. Stephens, R. W., L. Orning, H. Stormorken, M. Hamers, L. Petersen, K. Sakariassen: Characterisation of cell surface procoagulant activities using a microcarrier model. Thromb. Res. 84, 453-461 (1996).
31. Stolz, D. B., G. Bannish, B. S. Jacobson: The role of the cytoskeleton and intercellular junctions in the transcellular membrane potein polarity of bovine aortic endothelial cells in vitro. J. Cell Sci. 103, 53-68 (1992).
32. Takahashi, K., Y. Sawasaki, J. I. Hata, K. Mukai, T. Goto: Spontaneous transformation and immortalization of human endothelial cells. In Vitro Cell Dev. Biol. 25, 265-274 (1990).
33. Takahasi, K., Y. Sawasaki: Rare spontaneously transformed human endothelial cell line provides useful research tool. In Vitro Cell Dev. Biol. 28A, 380-382 (1992).
34. Villarete, L. H., D. G. Remick: Nitric oxide regulation of IL-8 expression in human endothelial cells. Biochem. Biophys. Res. Commun. 211, 671-676 (1995).